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pCag SE (Self-excising) FlpO-2A-Cre EV

Plasmid

#130986

Purpose

episomal expression of FlpO and Cre recombinases, self excising

Depositor

Joshua Breunig

Article

Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

Insert

FlpO-2A-Cre

Use

Cre/Lox and Unspecified; Episomal expression vect…

Expression

Mammalian

Mutation

protamine intron added to FlpO to prevent bacteri…

Promoter

CMV/Chick β-actin (CAG)

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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EFSp-GFP-YAP

Plasmid

#174168

Purpose

Lentiviral vector to constitutively express YAP under control of the EFS promoter.

Depositor

Rod Bremner

Article

Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610

Insert

YAP (YAP1 Human)

Use

Lentiviral

Tags

3x FLAG tag

Expression

Mammalian

Promoter

EFS

Available Since

Aug. 19, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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PGKp-GFP-YAP (S94A)

Plasmid

#174173

Purpose

Lentiviral vector to constitutively express TEAD-binding mutant YAP under control of the human PGK promoter.

Depositor

Rod Bremner

Article

Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610

Insert

YAP (YAP1 Human)

Use

Lentiviral

Tags

3x FLAG tag

Expression

Mammalian

Mutation

Serine 94 mutated to Alanine

Promoter

PGK

Available Since

Aug. 19, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pHPRT-DRGFP

Plasmid

#26476

Purpose

DR-GFP substrate (Addgene 26475) flanked by mouse hprt targeting sequences

Depositor

Maria Jasin

Article

Pierce et al Genes Dev. 2001 Dec 15. 15(24):3237-42.

Insert

pHPRT-DRGFP

Use

Mouse Targeting

Expression

Mammalian

Available Since

Nov. 3, 2010

Availability

Academic Institutions and Nonprofits only

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pCR3.1-OPNa

Plasmid

#107980

Purpose

expression of full-length OPN

Depositor

Georg Weber

Article

He et al Oncogene. 2006 Apr 6;25(15):2192-202. do

Insert

full-length osteopontin (SPP1 Human)

Expression

Mammalian

Available Since

May 10, 2018

Availability

Academic Institutions and Nonprofits only

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pCVL SFFV d14GFP Donor

Plasmid

#31475

Depositor

Andrew Scharenberg

Article

Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

Insert

SFFV d14GFP

Use

Lentiviral

Mutation

Deleted 14 amino acids on the c terminus of eGFP

Available Since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only

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Slug_pGL2

Plasmid

#31695

Depositor

Paul Wade

Article

Fujita et al Cell. 2003 Apr 18. 113(2):207-19.

Insert

Slug (SNAI2 Human)

Use

Luciferase

Expression

Mammalian

Available Since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pC.HA Vav2

Plasmid

#14554

Depositor

Joan Brugge

Article

Moores et al Mol Cell Biol. 2000 Sep . 20(17):6364-73

Insert

Vav2 (VAV2 Human)

Tags

HA

Expression

Mammalian

Available Since

April 12, 2007

Availability

Academic Institutions and Nonprofits only

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pCL20C-ddIPpoI

Plasmid

#49053

Purpose

Expresses the restriction enzyme IPpoI under the control of Shield1

Depositor

Michael Kastan

Article

Goldstein et al Proc Natl Acad Sci U S A. 2013 Sep 30.

Insert

DD-HA-ER-IPpoI

Use

Lentiviral

Available Since

Dec. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pC.HA Vav1

Plasmid

#14553

Depositor

Joan Brugge

Article

Moores et al Mol Cell Biol. 2000 Sep . 20(17):6364-73

Insert

Vav1 (VAV1 Human)

Tags

HA

Expression

Mammalian

Available Since

April 12, 2007

Availability

Academic Institutions and Nonprofits only

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p3993 pET ubcH7

Plasmid

#8654

Depositor

Peter Howley

Article

Scheffner et al Proc Natl Acad Sci U S A 1994 Sep 13;91(

Insert

UbcH7 (UBE2L3 Human)

Expression

Bacterial

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3_FLAG_MTA3

Plasmid

#31696

Depositor

Paul Wade

Article

Fujita et al Cell. 2003 Apr 18. 113(2):207-19.

Insert

Metastasis associated protein 3 (MTA3 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
pBabePuro-hSLUG.ER.NoTag

Plasmid

#19293

Depositor

Eric Fearon

Article

Hajra et al Cancer Res. 2002 Mar 15. 62(6):1613-8.

Insert

SLUG (SNAI2 Human)

Use

Retroviral

Tags

mouse ERalpha ligand-binding domain

Expression

Mammalian

Available Since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
pFSy(1.1)GW

Plasmid

#27232

Depositor

Pavel Osten

Article

Dittgen et al Proc Natl Acad Sci U S A. 2004 Dec 28. 1

Insert

EGFP

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Available Since

March 11, 2011

Availability

Academic Institutions and Nonprofits only

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pFCK(1.3)GW

Plasmid

#27230

Depositor

Pavel Osten

Article

Dittgen et al Proc Natl Acad Sci U S A. 2004 Dec 28. 1

Insert

EGFP

Use

Lentiviral

Tags

EGFP

Available Since

March 11, 2011

Availability

Academic Institutions and Nonprofits only

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pFCK(0.4)GW

Plasmid

#27229

Depositor

Pavel Osten

Article

Dittgen et al Proc Natl Acad Sci U S A. 2004 Dec 28. 1

Insert

EGFP

Use

Lentiviral

Tags

EGFP

Available Since

March 11, 2011

Availability

Academic Institutions and Nonprofits only

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pTYF-Tretight-DsRed2

Plasmid

#19983

Depositor

Sergey Kasparov

Article

Liu et al J Gene Med. 2008 May . 10(5):583-92.

Insert

Tretight-DsRed2

Expression

Mammalian

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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pRGM1

Plasmid

#48789

Purpose

HA-I-SceI expression plasmid, episomal

Depositor

Michael Glickman

Article

Gupta et al Mol Microbiol. 2011 Jan;79(2):316-30. do

Insert

I-SceI

Tags

HA tag

Expression

Bacterial

Promoter

MOP

Available Since

Jan. 31, 2014

Availability

Academic Institutions and Nonprofits only

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p3992 pET ubcH5

Plasmid

#8652

Depositor

Peter Howley

Article

Scheffner et al Proc Natl Acad Sci U S A 1994 Sep 13;91(

Insert

UbcH5 (UBE2D1 Human)

Expression

Bacterial

Available Since

July 18, 2005

Availability

Academic Institutions and Nonprofits only
pJKW1658

Plasmid

#119179

Purpose

E.coli expression plasmid for Pm4CL1 and PmSPS2

Depositor

Jing-Ke Weng

Article

Pluskal et al Nat Plants. 2019 Jul 22. pii: 10.1038/s4

Inserts

Pm4CL1
PmSPS2

Expression

Bacterial

Promoter

pGAP

Available Since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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