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pSB97 - pL2_pVS1_2x35S::fLUC-I::tNOS

Plasmid

#123189

Purpose

binary plant vector for transient fLUC (with intron) expression

Depositor

Erin Cram, Carolyn Lee-Parsons

Article

Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

Insert

2x35S::fLUC-I::tNOS

Use

Luciferase

Expression

Plant

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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Vh-HA-3xFRB

Plasmid

#20223

Depositor

Carolyn Bertozzi

Article

Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

Insert

3xFRB (MTOR Human)

Tags

HA and Vh

Expression

Mammalian

Mutation

One copy of FRB has a T2098L mutation

Available Since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
3xFRB-myc

Plasmid

#20226

Depositor

Carolyn Bertozzi

Article

Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

Insert

3xFRB (MTOR Human)

Tags

myc

Expression

Mammalian

Mutation

1 FRB copy has a T2098L point mutation

Available Since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
pLenti6/V5-DEST-UCHL5

Plasmid

#31219

Depositor

Lynda Chin

Article

Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

Insert

UCHL5 (UCHL5 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Aug. 19, 2011

Availability

Academic Institutions and Nonprofits only
pLenti6/V5-DEST-NDC80

Plasmid

#31215

Depositor

Lynda Chin

Article

Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

Insert

NDC80 (NDC80 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

July 29, 2011

Availability

Academic Institutions and Nonprofits only
Vh-HA-2xFRB

Plasmid

#20224

Depositor

Carolyn Bertozzi

Article

Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

Insert

2xFRB (MTOR Human)

Tags

HA and Vh

Expression

Mammalian

Mutation

One copy of FRB has a T2098L mutation

Available Since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
p164-pAAV-mDlx-FLEX-mScarlet

Plasmid

#204689

Purpose

Fluorescent reporter under the control of the Dlx promotor

Depositor

Lynette Lim

Article

Fisher et al Neuron. 2023 Dec 7:S0896-6273(23)00887-5

Insert

mScarlet

Use

AAV and Cre/Lox

Tags

mScarlet palmitoylation

Expression

Mammalian

Promoter

mDlx

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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2xFRB-myc

Plasmid

#20227

Depositor

Carolyn Bertozzi

Article

Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

Insert

2xFRB (MTOR Human)

Tags

myc

Expression

Mammalian

Mutation

1 FRB copy has a T2098L point mutation

Available Since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
pSB165 - pL1R3_pMAS::rLUC-I::tMAS

Plasmid

#123198

Purpose

binary plant vector for transient expression of a Renilla luciferase (rLUC, with intron)

Depositor

Erin Cram, Carolyn Lee-Parsons

Article

Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

Insert

pMAS::rLUC-I::tMAS

Use

Luciferase

Expression

Plant

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pUN378 - pTRV2-CrGLK

Plasmid

#203888

Purpose

Viral silencing plasmid targeting the CrGLK gene from C. roseus.

Depositor

Carolyn Lee-Parsons

Article

Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

Insert

CrGLK fragment

Use

RNAi

Expression

Plant

Available Since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

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pT7-hMLH1dn for IVT

Plasmid

#178114

Purpose

Template for in vitro transcription of human MLH1dn

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

human MLH1dn (codon optimized) (MLH1 Human)

Use

Template for in vitro transcription

Mutation

Detailed in manuscript

Promoter

T7 (inactivated)

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pSB117 - pL2_pSB90_fLUC-I_V2

Plasmid

#123192

Purpose

binary plant vector for transient TYLCV V2 and fLUC (with intron) expression

Depositor

Erin Cram, Carolyn Lee-Parsons

Article

Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

Insert

2x35S::V2::tNOS 2x35S::fLUC-I::tNOS

Use

Luciferase

Expression

Plant

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pSB90 - pAGM4723_VirGN54D

Plasmid

#123187

Purpose

Golden Gate (MoClo; Weber et al., 2011) Level 2 acceptor vector with increased virulence in Agrobacteria

Depositor

Erin Cram, Carolyn Lee-Parsons

Article

Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

Type

Empty backbone

Use

Level 2 acceptor vector for expression in plants

Expression

Plant

Mutation

VirGN54D for increase virulence

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti6/V5-DEST-ASF1B

Plasmid

#31205

Depositor

Lynda Chin

Article

Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

Insert

ASF1B (ASF1B Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

July 29, 2011

Availability

Academic Institutions and Nonprofits only
pTCB-nivoHCLALAPGknobHA

Plasmid

#237377

Purpose

pTwist CMV BetaGlobin encoding nivolumab heavy chain with LALA P329G knob Fc and HA tag

Depositor

Carolyn Bertozzi, Jessica Stark

Article

Stark et al Nat Biotechnol. 2025 Dec 16. doi: 10.103

Insert

nivolumab heavy chain (silent Fc)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pTCB-nivoLC

Plasmid

#237378

Purpose

pTwist CMV BetaGlobin encoding nivolumab light chain

Depositor

Carolyn Bertozzi, Jessica Stark

Article

Stark et al Nat Biotechnol. 2025 Dec 16. doi: 10.103

Insert

nivolumab light chain

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pIRES proHB-EGF WT

Plasmid

#11602

Depositor

Rosalyn Adam

Article

Adam et al Endocrinology. 2002 Dec . 143(12):4599-6

Insert

HB-EGF (HBEGF Human)

Expression

Mammalian

Available Since

April 26, 2006

Availability

Academic Institutions and Nonprofits only
pLenti-EF1a-hMLH1dn-Hygro

Plasmid

#195512

Purpose

Lentiviral expression plasmid of hMLH1dn with P2A-HygroR marker

Depositor

Hyongbum Kim

Article

Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

Insert

hMLH1dn-P2A-HygroR (MLH1 Human)

Use

Lentiviral

Tags

P2A

Expression

Mammalian

Mutation

Human MLH1 del754-756

Promoter

EF-1a

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pTCB-avelHCknob6xHis

Plasmid

#237383

Purpose

pTwist CMV BetaGlobin encoding avelumab heavy chain with knob Fc and 6xHis tag

Depositor

Carolyn Bertozzi, Jessica Stark

Article

Stark et al Nat Biotechnol. 2025 Dec 16. doi: 10.103

Insert

avelumab heavy chain

Tags

6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pTCB-avelLC

Plasmid

#237384

Purpose

pTwist CMV BetaGlobin encoding avelumab light chain

Depositor

Carolyn Bertozzi, Jessica Stark

Article

Stark et al Nat Biotechnol. 2025 Dec 16. doi: 10.103

Insert

avelumab light chain

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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