We narrowed to 2,587 results for: PGK

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pPB-CAG-Klf2.2A.Nanog-Cherry-pA-pgk-zeo

Plasmid

#74919

Purpose

Mammalian expression of mKlf2 and mNanog-mCherry

Depositor

Jose Silva

Article

dos Santos et al Cell Stem Cell. 2014 Jul 3;15(1):102-10.

Inserts

Klf2 (Klf2 Mouse)
Nanog (Nanog Mouse)

Tags

T2A and mCherry

Expression

Mammalian

Promoter

CAG

Available Since

May 26, 2016

Availability

Academic Institutions and Nonprofits only
MSCV-EZH2_EZH1SET-PGK-Puro-IRES-GFP

Plasmid

#75126

Purpose

Retroviral expression plasmid encoding a human EZH2 SET-domain-swap mutant containing the EZH1 SET domain

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

KMT6 (EZH2 Human)

Use

Retroviral

Tags

None

Mutation

SET domain replaced with the SET domain of EZH1

Available Since

May 23, 2016

Availability

Academic Institutions and Nonprofits only
pCCL-PGK-SPdCas9-BFP-DNMT3B(E697A)

Plasmid

#71214

Purpose

dCas9 fused to BFP and the human DNMT3B catalytically inactive domain

Depositor

Yonglun Luo

Article

Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

Insert

dSpCas9-BFP-DNMT3B(E697A), DNMT3B catalytic domain with inactivation mutation E697A

Tags

dSpCas9-BFP-DNMT3B(E697A)

Expression

Mammalian

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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plenti-UbcP-IKZF2-V2-3xHA-pGK-Pur

Plasmid

#69052

Purpose

lentiviral expression of IKZF2 variant 2 with HA tag

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

IKZF2 (IKZF2 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

splice variant 2

Promoter

UBC

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pLV-PGK-DIO-mCherry-TLR4miR-TLR2miR

Plasmid

#137733

Purpose

Lentivirus vector for TLR2/4 miR in a Cre-dependent manner

Depositor

Tomoyuki Furuyashiki

Article

Nie et al Neuron. 2018 Aug 8;99(3):464-479.e7. doi

Insert

TKR2/4 microRNA

Use

Lentiviral

Tags

mChherry

Expression

Mammalian

Available Since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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MSCV-BRD9_N216A-PGK-Puro-IRES-GFP

Plasmid

#75116

Purpose

Retroviral expression plasmid encoding a human BRD9 mutant carrying the N216A single amino acid mutation

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

BRD9 (BRD9 Human)

Use

Retroviral

Mutation

single amino acid mutation N216A

Available Since

May 18, 2016

Availability

Academic Institutions and Nonprofits only
MSCV-BRD9_BET-PGK-Puro-IRES-GFP

Plasmid

#75120

Purpose

Retroviral expression plasmid encoding a human BRD9 bromodomain-swap mutant containing the first bromodomain of BRD4

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

BRD9 (BRD9 Human)

Use

Retroviral

Mutation

Bromodomain replaced with the first bromodomain o…

Available Since

May 18, 2016

Availability

Academic Institutions and Nonprofits only
MDH1-miR-31SM-PGK-GFP-Blast

Plasmid

#31516

Depositor

Chang-Zheng Chen

Article

Arnold et al Genome Res. 2011 May . 21(5):798-810.

Insert

miR-31 (Mir31 Mouse)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

seed mutant control

Available Since

Sept. 22, 2011

Availability

Academic Institutions and Nonprofits only
pLenti-PGK-flag-RAP1B-V12-neo

Plasmid

#156175

Purpose

Lentiviral vector for expression of flag-RAP1B-V12, with neomycin selection

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

RAP1B (RAP1B Bovine)

Use

Lentiviral

Tags

2xFLAG

Expression

Mammalian

Mutation

changed Glycine 12 to Valine

Promoter

human PGK

Available Since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-flag-RAP1B-N17-neo

Plasmid

#156176

Purpose

Lentiviral vector for expression of flag-RAP1B-N17, with neomycin selection

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

RAP1B (RAP1B Bovine)

Use

Lentiviral

Tags

2xFLAG

Expression

Mammalian

Mutation

changed Serine 17 to Asparagine

Promoter

human PGK

Available Since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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pFH2.96_PB-EPN1-T2A-eGFP-PGK-Blast

Plasmid

#205541

Purpose

Express EPN1-T2A-eGFP and blasticidin resistance marker

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

EPN1

Use

Piggybac

Expression

Mammalian

Mutation

WT

Promoter

EF1

Available Since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pFH2.100_PB-EPN11-T2A-eGFP-PGK-Blast

Plasmid

#205545

Purpose

Express EPN11-T2A-eGFP and blasticidin resistance marker

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

EPN11

Use

Piggybac

Expression

Mammalian

Mutation

WT

Promoter

EF1

Available Since

Sept. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti PGK Puro GFP-GABARAPL1 G116A

Plasmid

#123245

Purpose

Expression vector with PGK promoter for low expression of EGFP-GABARAPL1 G116A. For lentivirus production and stable transduction in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Glycine 116 to Alanine

Promoter

PGK

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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MDH1-miR-222-PGK-GFP-Blast

Plasmid

#31522

Depositor

Chang-Zheng Chen

Article

Arnold et al Genome Res. 2011 May . 21(5):798-810.

Insert

miR-222 (Mir222 Mouse)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Available Since

Oct. 3, 2011

Availability

Academic Institutions and Nonprofits only
plenti-UbcP-FLAG-YWAA-pGK-HYG

Plasmid

#107375

Purpose

Lentiviral expression of FLAG-YWAA

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

cereblon (CRBN Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

CRBN-Y383A/W385A

Promoter

UbcP

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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MSCV-miR30_shBrd9_1116-PGK-NeoR-IRES-mCherry

Plasmid

#75133

Purpose

Retroviral expression plasmid encoding shBrd9_1116 (targeting murine Brd9)

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

shBrd9_1116

Use

Retroviral

Available Since

May 23, 2016

Availability

Academic Institutions and Nonprofits only
PB-PGK-FKBP-Ztetv-Cit-tvhc-DHFR

Plasmid

#116052

Purpose

Reporter plasmid for measuring protease activity

Depositor

Michael Elowitz

Article

Gao et al Science. 2018 Sep 21;361(6408):1252-1258

Insert

FKBP-Ztetv-Cit-tvhc-DHFR

Expression

Mammalian

Available Since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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plenti-UBC- IKZF5-3xHA-pGK-PUR

Plasmid

#107385

Purpose

Lentiviral expression of IKZF5-3xHA

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

IKZF5 (IKZF5 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

UBC

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-mNanog-mut-pgk-hph

Plasmid

#74914

Purpose

Mammalian expression of mNanog (Y42E, K43T)

Depositor

Jose Silva

Article

Theunissen et al Development. 2011 Nov;138(22):4853-65. d

Insert

Nanog (Nanog Mouse)

Expression

Mammalian

Mutation

K43T of Nanog protein and Y42E of Nanog homeodoma…

Promoter

CAG

Available Since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti-VQRRA-P2A-GFP-PGK-Puro

Plasmid

#110864

Purpose

Lentiviral vector for constitutive expression of Cas9-VQRRA-P2A-GFP in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-VQRRA

Use

Lentiviral

Tags

FLAG

Mutation

D1135V, R1335Q, T1337R and NLS sequence at the N-…

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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