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pYTK018

Plasmid

#65125

Purpose

Encodes pALD6 as a Type 2 part to be used in the Dueber YTK system

Depositor

John Dueber

Article

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

pALD6

Expression

Bacterial

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pYTK020

Plasmid

#65127

Purpose

Encodes pRET2 as a Type 2 part to be used in the Dueber YTK system

Depositor

John Dueber

Article

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

pRET2

Expression

Bacterial

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pYTK021

Plasmid

#65128

Purpose

Encodes pRNR1 as a Type 2 part to be used in the Dueber YTK system

Depositor

John Dueber

Article

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

pRNR1

Expression

Bacterial

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pYTK022

Plasmid

#65129

Purpose

Encodes pSAC6 as a Type 2 part to be used in the Dueber YTK system

Depositor

John Dueber

Article

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

pSAC6

Expression

Bacterial

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pRPR1_c2gRNA_RPR1t

Plasmid

#64380

Purpose

encodes c2 gRNA

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

c2 gRNA

Expression

Yeast

Available Since

May 5, 2015

Availability

Academic Institutions and Nonprofits only
pRPR1_g1gRNA_RPR1t

Plasmid

#64387

Purpose

encodes g1 gRNA

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

g1 gRNA

Expression

Yeast

Available Since

May 5, 2015

Availability

Academic Institutions and Nonprofits only
pRPR1_c7gRNA_RPR1t

Plasmid

#64385

Purpose

encodes c7 gRNA

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

c7 gRNA

Expression

Yeast

Available Since

May 5, 2015

Availability

Academic Institutions and Nonprofits only
pRPR1_c1gRNA_RPR1t

Plasmid

#64379

Purpose

encodes c1 gRNA

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

c1 gRNA

Expression

Yeast

Available Since

May 5, 2015

Availability

Academic Institutions and Nonprofits only
pRPR1_c5gRNA_RPR1t

Plasmid

#64383

Purpose

encodes c5 gRNA

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

c5 gRNA

Expression

Yeast

Available Since

May 5, 2015

Availability

Academic Institutions and Nonprofits only
pCAM36

Plasmid

#86476

Purpose

Expresses 6His-p9

Depositor

Pamela Silver

Article

Myhrvold et al ACS Synth Biol. 2016 Aug 3.

Insert

6His-p9

Use

Synthetic Biology

Tags

6His

Expression

Bacterial

Promoter

pTet

Availability

Academic Institutions and Nonprofits only

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pCAM53

Plasmid

#86477

Purpose

Expresses p12 and mCitrine-p9

Depositor

Pamela Silver

Article

Myhrvold et al ACS Synth Biol. 2016 Aug 3.

Insert

p12, mCitrine-p9

Use

Synthetic Biology

Tags

mCitrine

Expression

Bacterial

Promoter

pBAD

Availability

Academic Institutions and Nonprofits only

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E. coli BW25113 ΔtnaA ΔtrpR

Bacterial Strain

#205015

Purpose

Used for the characterization of the TrpR1-PtrpO1 biosensor system with the deletion of tnaA gene and trpR gene (no antibiotics marker)

Depositor

Yajun Yan

Article

Gong et al ACS Synth Biol. 2022 Jun 17;11(6):2175-2

Bacterial Resistance

None

Species

Escherichia coli

Available Since

Aug. 3, 2023

Availability

Industry, Academic Institutions, and Nonprofits
ATMY-C321

Bacterial Strain

#218774

Purpose

E. coli strain, genotype: C321.ΔA pUltraBR-MjY ΔtyrS tyrTV::tolC tyrU::GentR lambda::ZeoR

Depositor

Abhishek Chatterjee

Article

Ficaretta et al ACS Chem Biol. 2024 Jun 24. doi: 10.1021

Bacterial Resistance

Spectinomycin (50 ug/mL), Gentamycin (10 ug/mL)

Species

Escherichia coli

Available Since

June 28, 2024

Availability

Academic Institutions and Nonprofits only
JEC027

Bacterial Strain

#180310

Purpose

E. coli MG1655 strain with knockout of endogenous Type I-E CRISPR Cas system

Depositor

James Chappell

Article

Villegas Kcam et al ACS Synth Biol. 2022 Jan 25. doi: 10.102

Bacterial Resistance

None

Available Since

May 2, 2022

Availability

Academic Institutions and Nonprofits only
rEc.13.delA

Bacterial Strain

#69495

Purpose

Recoded strain from MG1655, prfA deleted

Depositor

Michael Jewett

Article

Hong et al ACS Synth Biol. 2014 Jun 20;3(6):398-409

Bacterial Resistance

Ampicillin

Available Since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only
pBCPB+

Plasmid

#18940

Depositor

Michele Calos

Article

Groth et al Proc Natl Acad Sci U S A. 2000 May 23. 9

Insert

attP, lacZ, attB

Expression

Bacterial

Available Since

Aug. 13, 2008

Availability

Academic Institutions and Nonprofits only

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pMito-HyPerGRt

Plasmid

#173197

Purpose

HyPer7 and tdTomato fusion to detect mitochondrial hydrogen peroxide

Depositor

Huiwang Ai

Article

Li et al ACS Sens. 2022 Feb 28. doi: 10.1021/acss

Insert

Hyper7-tdTomato

Expression

Mammalian

Promoter

CMV IE94 promoter

Available Since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBBR1_DVK_AE(J23100-BCD2-E0040m-B0015)

Plasmid

#197660

Purpose

Expresses GFP under the control of J23100 and BCD2

Depositor

Eric Young

Article

Keating et al ACS Synth Biol. 2023 Jun 9. doi: 10.1021

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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pSEVAT7K1A

Plasmid

#58302

Purpose

GeneGuard vector cassette: thyA, ColE2 COR, Kid toxin; with ampR as cargo.

Depositor

Tom Ellis

Article

Wright et al ACS Synth Biol. 2014 May 13.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pRIM3-rfp

Plasmid

#74073

Purpose

GentR version of pRIM2 carrying rfp

Depositor

Timothy Lu

Article

Cleto et al ACS Synth Biol. 2016 Feb 16.

Insert

rfp

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

ptac

Available Since

June 13, 2016

Availability

Academic Institutions and Nonprofits only

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