Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 3,842 results for: dcas9

Clear keywords
Showing: 1201 - 1220 of 3842 results

  1. KO101: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS-Dnmt3a3L

    Plasmid
    #121833
    Purpose
    pMAGIC R4-R3 entry plasmid, contains 2x NLS x-dCas9(3.7) fused to the Dnmt3a-3L DNA methyltransferase for 3 or 4-component MultiSite Gateway Pro assembly.
    Depositor
    Christopher Newgard
    Publication
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    x-dCas9(3.7)/Dnmt3a-3L (open)
    Use
    Gateway Cloning and Synthetic Biology
    Tags
    NLS
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121833-plasmid-map-sequence-id-237387

    Enlarge

  2. lenti SYN-SVI-DIO-dCas9-VPR

    Plasmid
    #164576
    Purpose
    Expresses a SYN-driven, intron-containing dCas9-VPR fusion in which the part of the dCas9 cassette is double floxed and in inverted orientation (DIO). Requires Cre-dependent recombination.
    Depositor
    Jeremy Day
    Publication
    Carullo et al eNeuro. 2021 Jul 26. pii: ENEURO.0188-21
    Insert
    A DIO FLAG-dCas9-VPR cassette containing an SV40 intron
    Use
    CRISPR, Cre/Lox, and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Available since
    Feb. 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    164576-plasmid-map-sequence-id-323657

    Enlarge

  5. pEGB 35S:dCas9:Tnos (GB1191)

    Plasmid
    #68223
    Purpose
    Transcriptional unit for human codon optimized with mutated (D10A, H840A) and inactivated catalytic domains Cas9 protein plant expression driven by the 35S promoter
    Depositor
    Diego Orzaez
    Publication
    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.
    Insert
    dCas9
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed; human codon optimis…
    Promoter
    35S
    Available since
    March 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    68223-plasmid-map-sequence-id-135584

    Enlarge

  19. pMD19T-psba1-TetR-PL22-dCas9-SpR

    Plasmid
    #73223
    Purpose
    Contains dCas9 from S. pyogenes under aTc inducible promoter. Suicide vector inserts into psba1 site of Synechocystis. Carries spectinomycin resist. Recommend E. coli Copy cutter for propogation.
    Depositor
    Paul Hudson
    Publication
    Yao et al ACS Synth Biol. 2015 Dec 28.
    Insert
    dCas9 from S. pyogenes
    Tags
    c-myc
    Expression
    Bacterial
    Mutation
    Silent mutation at bp 1341 A->C to remove an E…
    Promoter
    PL22
    Available since
    March 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    73223-plasmid-map-sequence-id-135055

    Enlarge

Showing: 1201 - 1220 of 3842 results