We narrowed to 6,650 results for: tac

1. poly E67R SUMO1

   Plasmid

   #221072

   Purpose

   Expression of poly E67R SUMO1 protein under T7 promoter

   Depositor

   Michael Rosen

   Article

   Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

   Insert

   Five repeats of E67R SUMO1 each separated by (GGS)4 (SUMO1 Human)
   

   Use

   Tags

   His and MBP

   Expression

   Bacterial

   Mutation

   E67R

   Promoter

   tac

   Available since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. poly H35K SUMO1

   Plasmid

   #221070

   Purpose

   Expression of poly H35K SUMO1 protein under T7 promoter

   Depositor

   Michael Rosen

   Article

   Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

   Insert

   Five repeats of H35K SUMO1 each separated by (GGS)4 (SUMO1 Human)
   

   Use

   Tags

   His and MBP

   Expression

   Bacterial

   Mutation

   H35K

   Promoter

   tac

   Available since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. poly H35R SUMO1

   Plasmid

   #221069

   Purpose

   Expression of poly H35R SUMO1 protein under T7 promoter

   Depositor

   Michael Rosen

   Article

   Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

   Insert

   Five repeats of H35R SUMO1 each separated by (GGS)4 (SUMO1 Human)
   

   Use

   Tags

   His and MBP

   Expression

   Bacterial

   Mutation

   H35R

   Promoter

   tac

   Available since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX4T3-GST-PolB(T304I)

   Plasmid

   #177133

   Purpose

   Bacterial vector for expression of an N-terminal GST fusion of PolB(T304I) with a TEV protease site located between the GST tag and PolB(T304I)

   Depositor

   Robert Sobol

   Article

   Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

   Insert

   PolB (POLB Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Tac

   Available since

   Sept. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX4T3-GST-PolB(K72A)

   Plasmid

   #177132

   Purpose

   Bacterial vector for expression of an N-terminal GST fusion of PolB(K72A) with a TEV protease site located between the GST tag and PolB(K72A)

   Depositor

   Robert Sobol

   Article

   Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

   Insert

   PolB (POLB Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Tac

   Available since

   July 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX4T3-GST-PolB(K206A/K244A)

   Plasmid

   #177135

   Purpose

   Bacterial vector for expression of an N-terminal GST fusion of PolB(K206A/K244A) with a TEV protease site located between the GST tag and PolB(K206A/K244A)

   Depositor

   Robert Sobol

   Article

   Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

   Insert

   PolB
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Tac

   Available since

   Dec. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMAL-c2X-ORF24-NTD-3L_A

   Plasmid

   #138466

   Purpose

   Expresses MBP-tagged ORF24-NTD 3L_A in bacteria

   Depositor

   Britt Glaunsinger

   Article

   Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

   Insert

   ORF24 (ORF24 KSHV (HHV-8))
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   Amino acids 1-201; amino acids L73-75 mutated to …

   Promoter

   tac

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX6p1 AO7R

   Plasmid

   #137842

   Purpose

   GST tagged AO7 encoding aa 126-216 for expression in bacteria

   Depositor

   Allan Weissman

   Article

   Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

   Insert

   AO7 (RNF25 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   March 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX-4T-3-cytXlATL2

   Plasmid

   #124063

   Purpose

   Fragment of Xenopus laevis Atlastin (cytATL). This fragment contains the GTPase domain and the three-helix bundle, but lacks the transmembrane segments and the cytosolic tail.

   Depositor

   Tom Rapoport

   Article

   Wang et al J Cell Biol. 2013 Dec 9;203(5):801-14. d

   Insert

   cytXIATL2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHTSHP-ShhN-Cys

    Plasmid

    #121136

    Purpose

    Expresses ShhN with a C-terminal cysteine residues in bacteria

    Depositor

    Philip Beachy

    Article

    Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

    Insert

    shh (Shh Mouse)
    

    Use

    Tags

    HIS, HRV3C site, and MBP

    Expression

    Bacterial

    Mutation

    Amino acid sequence from 26-191, with an addition…

    Promoter

    tac

    Available since

    March 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. TIAM2_PDZ_1

    Plasmid

    #103952

    Purpose

    Protein expression and purification of TIAM2 PDZ domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    TIAM2 (TIAM2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX-4T-1 mSesn2 (1190)

    Plasmid

    #66916

    Purpose

    bacterial expression of mouse Sesn2

    Depositor

    Jun Hee Lee, Michael Karin

    Article

    Park et al Nat Commun. 2014 Jun 20;5:4233. doi: 10.

    Insert

    Sesn2 (Sesn2 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Dec. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX4T-Mst2 SARAH

    Plasmid

    #84298

    Purpose

    Expresses Mst2 SARAH domain for interaction and spectroscopy studies.

    Depositor

    Jonathan Chernoff

    Article

    Rawat et al Curr Biol. 2016 Jun 20;26(12):1556-63. d

    Insert

    Mst2 (STK3 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    L443W

    Promoter

    tac

    Available since

    Oct. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pGEX-4T1 Erg (317-486)

    Plasmid

    #66992

    Purpose

    Bacterial expression plasmid for mouse Erg (317-486) with GST

    Depositor

    Christopher Vakoc

    Article

    Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

    Insert

    Erg (317-486) (Erg Mouse)
    

    Use

    Tags

    GST fusion

    Expression

    Bacterial

    Mutation

    Amino acids from 317-486

    Promoter

    TAC

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pGEX-4T1 Erg (209-316)

    Plasmid

    #66991

    Purpose

    Bacterial expression plasmid for mouse Erg (209-316) with GST

    Depositor

    Christopher Vakoc

    Article

    Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

    Insert

    Erg (209-316) (Erg Mouse)
    

    Use

    Tags

    GST fusion

    Expression

    Bacterial

    Mutation

    Amino acids from 209-316

    Promoter

    TAC

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pGEX-4T1 Erg (1-208)

    Plasmid

    #66990

    Purpose

    Bacterial expression plasmid for mouse Erg (1-208) with GST

    Depositor

    Christopher Vakoc

    Article

    Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

    Insert

    Erg (1-208) (Erg Mouse)
    

    Use

    Tags

    GST fusion

    Expression

    Bacterial

    Mutation

    Amino acids from 1-208

    Promoter

    TAC

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

17. RBMS2_2xRRM_pGEX

    Plasmid

    #135132

    Purpose

    Recombinant expression of RBMS2 with dual-affinity tag

    Depositor

    Christopher Burge

    Article

    Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

    Insert

    RBMS2 (RBMS2 Human)
    

    Use

    Tags

    GST-SBP

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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18. OA-1050U (cinnabar)

    Plasmid

    #132423

    Purpose

    express arrays of gRNA targeting Cinnabar under dU6-3 promoter

    Depositor

    Omar Akbari

    Article

    Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

    Insert

    cinnabar gRNA array
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    U6-3

    Available since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. Circular 200,100 (RAB7A)

    Plasmid

    #170118

    Purpose

    AAV vector carrying a guide RNA targeting the human RAB7A mRNA

    Depositor

    Prashant Mali

    Article

    Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

    Insert

    Circular 200,100 guide RNA (RAB7A Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLCHKO_TK1_HPRT1_Lb_6

    Plasmid

    #155064

    Purpose

    Lentiviral plasmid for expressing U6 driven multiplexed hybrid guide (hg)RNA targeting TK1 using SpCas9 and five intergenic sites & HPRT1 using LbCas12a (CHyMErA system)

    Depositor

    Benjamin Blencowe, Jason Moffat

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA targeting TK1 using SpCas9 and five intergenic sites & HPRT1 using LbCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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