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Plasmid#136134PurposePlasmid to transform Mp with constitutive promoter marking cell outline with eGFP. HygRDepositorInsertp5-35S:HygR p5-MpUBE2:eGFP-Lti6b
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
L2_UBE2:mT-N7-CsA
Plasmid#136133PurposePlasmid to transform Mp with constitutive promoter driving mTurq to the nuclei. HygRDepositorInsertp5-35S:HygR p5-MpUBE2:mTurq-N7
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAGM55273
Plasmid#153211PurposeLevel 2 cloning vector for a single guide RNA, already contains Cas9, Kanamycin selection cassette for plant transformation and FAST markerDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAM-kan-R4R3
Plasmid#71275PurposeBinary destination vector for plant transformation. Kanamycin resistance.DepositorTypeEmpty backboneUseMultisite gatewayTagsExpressionMutationPromoterAvailable sinceDec. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAGM65905
Plasmid#153217PurposeLevel 2 cloning vector for one or several guide RNAs, already contains Cas9 and a kanamycin selection cassette for plant transformationDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pH-A3A-PBE
Plasmid#119774PurposeConversion of C to T in rice plants transformationDepositorInsertAPOBEC3A-nCas9-NLS-UGI-NLS
UseTagsNLS (flanking UGI)ExpressionPlantMutationnCas9 has a mutation D10A in wild type SpCas9Promotermaize Ubiquitin-1Available sinceSept. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET15bHis6Tnp
Plasmid#79807PurposeTransform BL21 Gold(DE3) to induce the expression of HisTnp hyperactive mutationsDepositorInsertHis6Tnp
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAP01
Plasmid#59981PurposeThe function of this material is to facilitate the capture and expression of bacterial gene clusters.DepositorTypeEmpty backboneUseSynthetic Biology; Transformation-associated reco…TagsnoExpressionBacterial and YeastMutationPromoternoAvailable sinceNov. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ALK5 K232R
Plasmid#80878Purposemammalian expression of ALK5 K232RDepositorInsertALK5 (TGFBR1 Human)
UseTagsHAExpressionMammalianMutationK232R (kinase dead)PromoterCMVAvailable sinceAug. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAGM55297
Plasmid#153213PurposeLevel 2 cloning vector for a single guide RNA, already contains Cas9, Kanamycin selection cassette for plant transformation and FAST markerDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-TGFα-GSY-SPM
Plasmid#163615PurposeExpresses mature human Transforming Growth Factor alpha fused to SPM in bacterial cells for calcium-induced covalent coupling to Epidermal Growth Factor Receptor.DepositorInsertTGFa-GSY-SPM (TGFA Synthetic, Human)
UseTagsC-tag and His6ExpressionBacterialMutationPromoterT7Available sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAGM65881
Plasmid#153215PurposeLevel 2 cloning vector for one or several guide RNAs, already contains Cas9 and a kanamycin selection cassette for plant transformationDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJC30X-BS(X)
Plasmid#58958PurposeEncodes synthetic mitochondrial gene GFPm-3 in place of COX3 coding sequence. Flanked by mtDNA sequences allowing integration into rho+ mtDNA after transformation.DepositorInsertEnhanced fluorescence variant of GFP, in S. cerevisiae mitochondrial genetic code
UseTagsFirst 8 codons of COX3ExpressionMutationGFP modified as described: CORMACK, B. P., R. H. …Promotern/aAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 TGFB1
Plasmid#67080PurposeStandard for cell-free expression benchmarking.DepositorInsertTGFB1 (TGFB1 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
HS-DN-Ect2
Plasmid#105956Purposeheat shock inducible transgenesisDepositorInsertEct2 (ECT2 Human)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBI101.1/ProGOXL1:GUS
Plasmid#175557PurposeBinary vector for Agrobacterium-mediated plant transformation - Promoter:GUS reporter for expression analysis of GOXL genesDepositorInsertProGOXL1
UseTagsGUSExpressionPlantMutationPromoterGOXL1Available sinceJan. 13, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEN_TT Tgfbr3-HA
Plasmid#58497PurposeGateway entry vector for an inducible HA-tagged mouse Tgfbr3DepositorInsertTgfbr3_HA (Tgfbr3 Mouse)
UseEntry vectorTagsHAExpressionMutationPromoterAvailable sinceAug. 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
5mCUC1 pGreenII0129
Plasmid#12067DepositorInsertCUC1 mutant (CUC1 Mustard Weed)
UsePlant transformation via agrobacteriumTagsExpressionMutationmiR-resistant CUC1. Made by introducing five sile…PromoterAvailable sinceJune 2, 2006AvailabilityAcademic Institutions and Nonprofits only -
pEF-HA/Net1 Δ1-121
Plasmid#28263DepositorInsertNet1 (Net1 Mouse)
UseTags2x HAExpressionMammalianMutationMissing aa 1-121PromoterAvailable sinceApril 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET24a_SMOX
Plasmid#186345PurposeC-terminal His-Tag and a TEV cleaveage site, to transform into E. coli BL21 (DE3) cellsDepositorInsertSpermine oxidase (SMOX Human)
UseTagsHis tagExpressionBacterialMutationPromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only