Addgene

We narrowed to 12,245 results for: alp;

Showing: 1221 - 1240 of 12245 results

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pNTPTP alpha (S180A/S204A)

Plasmid

#17700

Depositor

Xinmin Zheng

Article

Zheng et al J Biol Chem. 2002 Jun 14. 277(24):21922-

Insert

PTPalpha S180A S204A (PTPRA Human)

Use

Tags

Expression

Mammalian

Mutation

S180A / S204A

Promoter

Available since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
pEGB2alpha2 35s:hCas9:tNos (GB0639)

Plasmid

#68222

Purpose

Transcriptional unit for (human codon optimized) Cas9 plant expression driven by the 35S promoter

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

hCas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

35S

Available since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-nAChr-Alpha6-GFP

Plasmid

#50486

Purpose

this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF

Depositor

Henry Lester

Article

Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

Insert

nAChr-alpha6 (Chrna6 Mouse)

Use

Tags

GFP fusion in M3-M4 loop (after residue A405)

Expression

Mammalian

Mutation

Gly-Ala-Gly flexible linker flanking the GFP open…

Promoter

CMV

Available since

Jan. 13, 2014

Availability

Academic Institutions and Nonprofits only
pAAV-EF1alpha-soCoChR-GFP

Plasmid

#107709

Purpose

A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. EF1alpha promoter

Depositor

Edward Boyden

Article

Shemesh et al Nat Neurosci. 2017 Dec;20(12):1796-1806.

Insert

soCoChR-GFP

Use

AAV

Tags

EGFP and KA2

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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XE242 Mouse CK2alpha CS2p+

Plasmid

#16730

Depositor

Randall Moon

Article

Waxman et al Development. 2004 Dec. 131(23):5909-21.

Insert

mouse Casein Kinase II alpha-pCS2P+ (Csnk2a1 Mouse)

Use

Xenopus expression

Tags

Expression

Mutation

Promoter

Available since

Sept. 12, 2012

Availability

Academic Institutions and Nonprofits only
pCMV5 Flag p38 alpha

Plasmid

#20786

Depositor

Roger Davis

Article

Raingeaud et al J Biol Chem. 1995 Mar 31. 270(13):7420-6

Insert

pCMV5 Flag p38 alpha (Mapk14 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

April 14, 2009

Availability

Academic Institutions and Nonprofits only
pFastBac1-OSF-TRIM5alpha(rhesus)

Plasmid

#79033

Purpose

Baculoviral entry vector to produce Bac-to-bac baculoviruses

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

OSF-TRIM5alpha(rhesus)

Use

Tags

OneSTrEP-FLAG

Expression

Insect

Mutation

Promoter

polyhedrin

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pscALPS-SIV-WRC-vpr

Plasmid

#115839

Purpose

Encodes codon optimized 3xFLAG-SIVWRC Vpr (AM713177) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVWRC Vpr (AM713177)

Use

Lentiviral

Tags

3x-FLAG

Expression

Mutation

Promoter

SFFV

Available since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pPD1025 EF1alpha TetON3G in TU6

Plasmid

#139262

Purpose

Expresses TetON3G under hEF1alpha in TUPV6

Depositor

Joshua Leonard

Insert

TetON3G

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

hEF1alpha

Available since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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LV_EF1a_ERalpha(Y537S)-P2A-Hygro_Barcode

Plasmid

#170225

Purpose

Barcoded lentiviral vector to express ERalpha (Y537S) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

ER (ESR1 Human)

Use

Lentiviral

Tags

V5

Expression

Mutation

Y537S

Promoter

EF1a

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pCRII-TOPO GFR alpha 1

Plasmid

#119898

Purpose

Mouse GFR alpha 1 In situ hybridization probe

Depositor

David Ginty, Wenqin Luo

Article

Luo et al Neuron. 2009 Dec 24;64(6):841-56. doi: 1

Insert

GDNF family receptor alpha-1 precursor (Gfra1 Mouse)

Use

In situ hybridization probe

Tags

Expression

Mutation

Promoter

Available since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pNL-EGFP/EF1alpha/WPREdU3

Plasmid

#18698

Depositor

Jakob Reiser

Article

Ricks et al Stem Cells Dev. 2008 Jun . 17(3):441-50.

Insert

EGFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 27, 2008

Availability

Academic Institutions and Nonprofits only

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pGEM-rat FceRI alpha

Plasmid

#8373

Depositor

Jean Kinet

Article

Kinet et al Biochemistry 1987 Jul 28;26(15):4605-10.

Insert

rat Fc epsilon RI alpha cDNA (Fcer1a Rat)

Use

Beta-gal

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 7, 2005

Availability

Academic Institutions and Nonprofits only
pDGB3 alpha1 P35S:AsCas12a:T35S (GB1440)

Plasmid

#160610

Purpose

Transcriptional unit for the expression of the AsCas12a in plant systems driven by the CaMV35s promoter.

Depositor

Diego Orzaez

Article

Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

Insert

P35s:AsCas12a:T35s

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

P35S

Available since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pscALPS-SIV-GRV vpr

Plasmid

#115827

Purpose

Encodes codon optimized 3xFLAG-SIVGRV Vpr (M66437) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVGRV Vpr (M66437)

Use

Lentiviral

Tags

3x-FLAG

Expression

Mutation

Promoter

SFFV

Available since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pscALPS-SIV-DEN vpr

Plasmid

#115829

Purpose

Encodes codon optimized 3xFLAG SIVDEN Vpr (AJ580407) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVDEN Vpr (AJ580407)

Use

Lentiviral

Tags

3x-FLAG

Expression

Mutation

Promoter

SFFV

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pscALPS-SIV-ASC vpr

Plasmid

#115824

Purpose

Encodes codon optimized 3xFLAG-SIVASC Vpr (KJ461715) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVASC Vpr (KJ461715)

Use

Lentiviral

Tags

3x-FLAG

Expression

Mutation

Promoter

SFFV

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pscALPS-SIV-DEB vpr

Plasmid

#115823

Purpose

Encodes codon optimized 3xFLAG-SIVDEB Vpr (FJ919724) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVDEB Vpr (FJ919724)

Use

Lentiviral

Tags

3x-FLAG

Expression

Mutation

Promoter

SFFV

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pscALPS-SIV-LST vpr

Plasmid

#115818

Purpose

Encodes codon optimized 3xFLAG-SIVLST Vpr (AF188116) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVLST Vpr (AF188116)

Use

Lentiviral

Tags

3x-FLAG

Expression

Mutation

Promoter

SFFV

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pscALPS-SIV-MND1 vpr

Plasmid

#115817

Purpose

Encodes codon optimized 3xFLAG-SIVMND1 Vpr (M27470) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVMND1 Vpr (M27470)

Use

Lentiviral

Tags

3x-FLAG

Expression

Mutation

Promoter

SFFV

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge