We narrowed to 4,519 results for: gca

1. pLsCas13a-gRNA-2

   Plasmid

   #164866

   Purpose

   Constitutive expression of single-spacer CRISPR array with spacer #2 targeting deGFP mRNA for LsCas13a in bacteria.

   Depositor

   Chase Beisel

   Article

   Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

   Insert

   LsCas13a-gRNA-2
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119

   Available since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. PLKO.1-UPF3A-3'UTR

   Plasmid

   #136042

   Purpose

   UPF3A shRNA (Targeting 3'UTR) inserted into the PLKO.1 plasmid (GACGTAGAAACACGCAGAAAC)

   Depositor

   Anthony Leung

   Article

   Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

   Insert

   UPF3A (UPF3A Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. PLKO.1-UPF3A-CDS

   Plasmid

   #136043

   Purpose

   UPF3A shRNA (Targeting CDS) inserted into the PLKO.1 plasmid (GCAGAAACCATTCTAAAGAAA)

   Depositor

   Anthony Leung

   Article

   Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

   Insert

   UPF3A (UPF3A Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pALPS puro miR30-IRF7

   Plasmid

   #117158

   Purpose

   Target site ATAAGGAAGCACTCGATGTCG

   Depositor

   Jeremy Luban

   Article

   McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

   Insert

   shRNA targeting miR30
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. EMX1*_sgRNA

   Plasmid

   #100558

   Purpose

   Expresses EMX1* sgRNA. Target sequence: (G)GAGTCCGAGCAGAAGAAGAA. Same target sequence as #100555, except with an additional 5' G.

   Depositor

   Dustin Maly

   Article

   Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

   Insert

   EMX1 sgRNA
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Sept. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. PX458_ZNF792_1

   Plasmid

   #86339

   Purpose

   Encodes gRNA for 3' target of human ZNF792

   Depositor

   Richard M. Myers, Eric Mendenhall

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   gRNA against ZNF792 (ZNF792 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAN-PBAD-sgRNA-VR

   Plasmid

   #62286

   Purpose

   expression of VR sgRNA from the arabinose-inducible promoter

   Depositor

   Christopher Voigt

   Article

   Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

   Insert

   VR sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available since

   April 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. MsMUT_sgRNA1

   Plasmid

   #111296

   Purpose

   CRISPR KO murine MUT

   Depositor

   Vamsi Mootha

   Article

   Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

   Insert

   sgRNA1 against murine MUT
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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9. 7b sgRNA for EJ7-GFP reporter

   Plasmid

   #113624

   Purpose

   sgRNA/CAS9 expression plasmid to induce the 3’ double-strand break in the EJ7-GFP reporter

   Depositor

   Jeremy Stark

   Article

   Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

   Insert

   7b sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

10. 7h sgRNA for 4-μHOM reporter

    Plasmid

    #113625

    Purpose

    sgRNA/CAS9 expression plasmid to induce a 3’ double-strand break in the 4-μHOM reporter at the edge of the microhomology.

    Depositor

    Jeremy Stark

    Article

    Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

    Insert

    7h sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

11. pU6-PspCas13b-GEMSgRNA2

    Plasmid

    #204768

    Purpose

    pU6-PspCas13b-gRNA-Actb1216 backbone (Addgene ID: 155368) containing guide RNA #2 targeting the GEMS m6A reporter mRNA sequence.

    Depositor

    Kate Meyer

    Article

    Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    guide RNA targeting GEMS m6A sensor mRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. TBP6.7 non-targeting gRNA lambda phage

    Plasmid

    #132547

    Purpose

    expresses gRNA for TBP-based CIRTS

    Depositor

    Bryan Dickinson

    Article

    Rauch et al Cell. 2019 Jun 27;178(1):122-134.e12. do

    Insert

    gRNA TBP-based CIRTS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6 promoter

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. SlutrCas9

    Plasmid

    #163795

    Purpose

    Expresses SlutrCas9, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Article

    Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBa.Lamp1-GFP

    Plasmid

    #180250

    Purpose

    Mammalian expression of LAMP1

    Depositor

    Marvin Bentley

    Article

    Nabb et al Mol Biol Cell. 2021 Nov 3:mbcE21080425.

    Insert

    LAMP1 (LAMP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Chicken Beta actin

    Available since

    March 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV.GfaABC1D.PI.Lck-GFP.SV40

    Plasmid

    #105598

    Purpose

    AAV expression of membrane bound EGFP from GfaABC1D promoter

    Depositor

    Baljit Khakh

    Article

    Shigetomi et al J Gen Physiol. 2013 Apr 15.

    Has Service

    AAV5

    Insert

    Lck-EGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    GfaABC1D

    Available since

    March 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLKO-shBRCA1 #2

    Plasmid

    #44595

    Depositor

    Eros Lazzerini Denchi

    Article

    Okamoto et al Nature. 2013 Feb 6. doi: 10.1038/nature1

    Insert

    BRCA1 (Brca1 Mouse)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pPU-huTRIMmiR30-TRIM5

    Plasmid

    #132938

    Purpose

    All-in-one huTRIM5 rescue-TRIM5 shRNA knockdown

    Depositor

    Jeremy Luban

    Article

    Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

    Insert

    human TRIM5
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Oct. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. DNA-PKcs N-terminal sgRNA

    Plasmid

    #207093

    Purpose

    pX330 based plasmid for expression of Cas9 and the AGCGGGACTCGGCGGCATGG sgRNA to target the DNA-PKcs locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    AGCGGGACTCGGCGGCATGG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. sgRNA 2 GAPDH

    Plasmid

    #83809

    Purpose

    Express Sg-2 sgRNA targeting GAPDH

    Depositor

    Bo Feng

    Article

    He et al Nucleic Acids Res. 2016 May 19;44(9):e85

    Insert

    SgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. LentiGuidPuro-hTP73_sgRNA-1

    Plasmid

    #88855

    Purpose

    CRISPR KO of Trp73

    Depositor

    Joan Massague

    Article

    Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

    Insert

    Trp73
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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