We narrowed to 2,560 results for: tk

1. pAWSV-17

   Plasmid

   #72770

   Purpose

   pBT264-[miR-196]

   Depositor

   Timothy Lu

   Article

   Wong et al Nat Biotechnol. 2015 Sep;33(9):952-61. d

   Insert

   [miR-196]
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAWSV-19

   Plasmid

   #72772

   Purpose

   pBT264-[miR-211]

   Depositor

   Timothy Lu

   Article

   Wong et al Nat Biotechnol. 2015 Sep;33(9):952-61. d

   Insert

   [miR-211]
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAWSV-21

   Plasmid

   #72774

   Purpose

   pBT264-[miR-216]

   Depositor

   Timothy Lu

   Article

   Wong et al Nat Biotechnol. 2015 Sep;33(9):952-61. d

   Insert

   [miR-216]
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAWSV-32

   Plasmid

   #72785

   Purpose

   pBT264-[miR-429]

   Depositor

   Timothy Lu

   Article

   Wong et al Nat Biotechnol. 2015 Sep;33(9):952-61. d

   Insert

   [miR-429]
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAWSV-34

   Plasmid

   #72787

   Purpose

   pBT264-[miR-488]

   Depositor

   Timothy Lu

   Article

   Wong et al Nat Biotechnol. 2015 Sep;33(9):952-61. d

   Insert

   [miR-488]
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAWSV-1

   Plasmid

   #72754

   Purpose

   pBT264-[let-7a]

   Depositor

   Timothy Lu

   Article

   Wong et al Nat Biotechnol. 2015 Sep;33(9):952-61. d

   Insert

   [let-7a]
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. mU6-CR1-CRISPRiv2_hU6-CR3-EMC2-guide_EF1a-PuroR-T2A-BFP

   Pooled Library

   #197349

   Purpose

   To allow for genome-wide genetic modifier screening, the library has a second fixed guide targeting EMC2.

   Depositor

   Jonathan Weissman, Rebecca Voorhees

   Article

   Guna et al BMC Genomics. 2023 Oct 30;24(1):651. doi

   Species

   Homo sapiens

   Use

   Lentiviral

   Available since

   May 25, 2023

   Availability

   Academic Institutions and Nonprofits only

8. pAG26

   Plasmid

   #226743

   Purpose

   Expresses a reporter for a mitochondrial intermembrane space protein

   Depositor

   Jonathan Weissman

   Article

   Guna et al Science. 2022 Oct 21;378(6617):317-322.

   Insert

   MICU1 (targeting sequence) (MICU1 Human)
   

   Use

   Lentiviral

   Tags

   GFP1-10

   Expression

   Mutation

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAG491

   Plasmid

   #226747

   Purpose

   CRISPRi guide RNA against MTCH2 (dual guide)

   Depositor

   Jonathan Weissman

   Article

   Guna et al Science. 2022 Oct 21;378(6617):317-322.

   Insert

   MTCH2 (MTCH2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGM234

    Plasmid

    #220177

    Purpose

    Expresses exogenous wild-type NAC complex members (NACA and BTF3)

    Depositor

    Jonathan Weissman

    Article

    Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

    Insert

    NACA and BTF3 (NACA, BTF3 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG (NACA); 6xHis (BTF3)

    Expression

    Mutation

    Promoter

    Available since

    June 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. CMVp-dsRed2-Triplex-28-gRNA3-28-gRNA4-28-gRNA5-28-gRNA6-28-pA

    Plasmid

    #55202

    Purpose

    Plasmid encoding multiplexed 4x gRNAs. This is a modified form of the original plasmid described in the paper (Construct 19). mKate2 was replaced with dsRed2 because of distribution issues.

    Depositor

    Timothy Lu

    Article

    Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

    Insert

    dsRed2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCDFNKI-strepII-3C-LIC-strep

    Plasmid

    #108708

    Purpose

    Expression of a StrepII-tagged target protein with 3C protease cleavage site.

    Depositor

    Titia Sixma

    Article

    Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

    Type

    Empty backbone

    Use

    Tags

    StrepII-3C protease cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. CMVp-dCas9-3xNLS-VP64 (Construct 1)

    Plasmid

    #55195

    Purpose

    Expresses taCas9 in Mammalian cells for transactivating endogenous and synthetic promoters. The backbone is a lentiviral vector.

    Depositor

    Timothy Lu

    Article

    Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

    Insert

    dCas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    VP64

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/hUBC

    Available since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pETNKI-strepII-3C-LIC-Amp

    Plasmid

    #108707

    Purpose

    Expression of a StrepII-tagged target protein with 3C protease cleavage site.

    Depositor

    Titia Sixma

    Article

    Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

    Type

    Empty backbone

    Use

    Tags

    StrepII-3C protease cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGEX6p1-GST-Rnf31

    Plasmid

    #63134

    Purpose

    Expression of human RNF31/HOIP codon optimized for E. coli and insect cells

    Depositor

    Titia Sixma

    Article

    Smit et al EMBO J. 2012 Oct 3;31(19):3833-44. doi:

    Insert

    Rnf31 (RNF31 Synthetic, Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Synthetic based on human protein sequence

    Promoter

    tac

    Available since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

16. Myc-RILPL1

    Plasmid

    #201470

    Purpose

    For overexpression in cultured cells to visualize RILPL1 protein interactions.

    Depositor

    Suzanne Pfeffer

    Article

    Pal et al Sci Adv. 2023 Dec 15;9(50):eadj1205. doi

    Insert

    RILPL1 (RILPL1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMM643

    Plasmid

    #112533

    Purpose

    pZE2-PLhrtO-lux-hrtR-RBS3-chuA - HrtR RBS variant of plasmid pMM627 (Strength 33545.5 AU), ColE1 origin, KanR

    Depositor

    Timothy Lu

    Article

    Mimee et al Science. 2018 May 25;360(6391):915-918.

    Inserts

    HrtR
    ChuA
    luxCDABE
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A17P

    Promoter

    J23107, PL(HrtO), and ProD

    Available since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCDNA3.1-Flag-H2A K13-15-118-119R

    Plasmid

    #63568

    Purpose

    Expression of human H2A (with multiple Lysine to Arginine point mutations) tagged with Flag

    Depositor

    Titia Sixma

    Article

    Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

    Insert

    H2A (H2AC20 Human)
    

    Use

    Tags

    Flag (MDYKDDDDKVPRIQ)

    Expression

    Mammalian

    Mutation

    Lysine at 13, 15, 118, and 119 are now Arginine

    Promoter

    CMV

    Available since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pCDNA3.1-Flag-H2A K5-9-118-119-125-127-129R

    Plasmid

    #63565

    Purpose

    Expression of human H2A (with multiple Lysine to Arginine point mutations) tagged with Flag

    Depositor

    Titia Sixma

    Article

    Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

    Insert

    H2A (H2AC20 Human)
    

    Use

    Tags

    Flag (MDYKDDDDKVPRIQ)

    Expression

    Mammalian

    Mutation

    Lysines at 5, 9, 118, 119, 125, 127, and 129 are…

    Promoter

    CMV

    Available since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

20. SpyTag-RBD

    Plasmid

    #159999

    Purpose

    Expresses SARS-CoV-2 RBD (340-538) (receptor-binding domain from Spike) with N-terminal SpyTag fusion in mammalian cells

    Depositor

    Mark Howarth

    Article

    Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

    Insert

    SpyTag-SARS-CoV-2 RBD (340-538) (S SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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