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pLX317 PRDM14

Plasmid

#193682

Purpose

Constitutive lentiviral expression of PRDM14

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

PRDM14 (PRDM14 Human)

Use

Lentiviral

Available Since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(0)-T2A-3X Gal80-Hsp70

Plasmid

#62951

Purpose

Creating Gal80 lines from MiMIC lines with inserts into coding introns in Phase 0. Gal80 is tagged with FLAG, HA and EGFP.

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

3XGal80-FLAG/HA/EGFP-Hsp70

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti_Blast_DEST mouse_p65promoter_mouse_p65-mRuby2

Plasmid

#124903

Purpose

Lentiviral vector to express p65-mRuby2 under p65 promoter (With blasticidin resistance)

Depositor

Markus Covert

Article

Lane et al Cell Syst. 2019 Mar 27;8(3):183-196.e4.

Insert

p65 promoter- p65 CDS-mRuby2

Use

Lentiviral

Tags

mRuby

Expression

Mammalian

Available Since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-NRCH-PE2-BSD

Plasmid

#191101

Purpose

Lentiviral expression plasmid of NRCH-PE prime editor with P2A-BSD marker

Depositor

Hyongbum Kim

Article

Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

Insert

NRCH-PE2-BSD

Use

CRISPR and Lentiviral

Tags

SV40 NLS

Expression

Mammalian

Promoter

EF-1a

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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5HT6-YFP-Inp54p(D281A)

Plasmid

#96813

Purpose

phosphatase-dead control for 5HT6-YFP-Inp54p

Depositor

Takanari Inoue

Article

Phua et al Cell. 2017 Jan 12;168(1-2):264-279.e15.

Insert

5HT6-YFP-Inp54p(D281A)

Tags

YFP

Expression

Mammalian

Mutation

D281A inactivates phosphatase activity

Available Since

Sept. 1, 2017

Availability

Academic Institutions and Nonprofits only

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vYM082_PB-pEF1s(min)-3xNLS-Citrine-T2A-iaaH(Hygro)

Plasmid

#160044

Purpose

For expressing iaaH to convert auxin precursors (IAM or NAM) to auxin (IAA or NAA)

Depositor

Michael Elowitz

Article

Ma et al Cell. 2022 Mar 17;185(6):967-979.e12. do

Insert

iaaH (iaaH )

Use

Synthetic Biology

Tags

3xNLS-Citrine

Expression

Mammalian

Promoter

pEF1s

Available Since

April 11, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR-PKAKTRClover

Plasmid

#59146

Purpose

ORF encoding PKA KTR mClover flanked by gateway sequences

Depositor

Markus Covert

Article

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

PKA Kinase Translocation Reporter (PRKACA Mouse, Human)

Available Since

Sept. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pMXs-Runx1

Plasmid

#131609

Purpose

expression of mouse Runx1 in mammalian cells

Depositor

Kateri Moore, Filipe Pereira

Article

Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

Insert

Runx1 (Runx1 Mouse)

Use

Retroviral

Expression

Bacterial

Available Since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pSSA-RPG

Plasmid

#85932

Purpose

Constitutively express DsRed and conditionally express Puro and EGFP. Used to test transfection efficiency and enrich genetically modified cells

Depositor

Zhiying Zhang

Article

Ren et al Cell Mol Life Sci. 2015 Jul;72(14):2763-

Insert

pPB-CMV-DsRed-polyA-CAG-SSA. Puro-T2A-EGFP

Use

Dual-reporter surrogate

Expression

Mammalian

Available Since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_SNIPR_hsNotch1 LNR, Notch2 JMD

Plasmid

#188374

Purpose

Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a Notch1-ECD, a Notch1-TMD, a Notch2-JMD, and a Gal4VP64 transcriptional factor

Depositor

Kole Roybal

Article

Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

Insert

PGK_antiCD19_Notch1-ECD_Notch1-TMD_Notch2-JMD_Gal4VP64

Use

Lentiviral

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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