Addgene

We narrowed to 4,404 results for: ARA-2

Showing: 1241 - 1260 of 4404 results

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pFW2100

Plasmid

#224212

Purpose

Bacteroides genomic editing tool; IPTG inducible-Fncpf1

Depositor

Ophelia Venturelli

Article

Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

Insert

Fncpf1

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pWM_12x601_193 NRL + TetO

Plasmid

#157812

Purpose

EcoRV digestion produces 12x601 chromatin assembly construct with central TetO in addition to carrier DNA that prevents overassembly of nucleosomes

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

12x601_tetO

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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pSLS.491

Plasmid

#184956

Purpose

Test effect of a1/a2 length on editing rpoB using retron recombineering

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1 RT and recombineering ncRNA, rpoB T1534C, a1/a2 length: 12

Use

Tags

Expression

Bacterial

Mutation

rpoB donor T1534C

Promoter

T7/lac

Available since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pFW5000

Plasmid

#224214

Purpose

E. coli-Bacteroides shuttle plasmid; pB8-51 (Bacteroides replication origin), p15A (E. coli replication origin), tetQ

Depositor

Ophelia Venturelli

Article

Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pFW6000

Plasmid

#224215

Purpose

Bacteroides genomic editing tool; aTc inducible-Fncpf1

Depositor

Ophelia Venturelli

Article

Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

Insert

Fncpf1

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pAcGFP1-N1-MAHS

Plasmid

#90034

Purpose

To express GFP-tagged MAHS in mammalian cells transiently

Depositor

Takekazu Kunieda

Article

Tanaka et al PLoS One. 2015 Feb 12;10(2):e0118272. eC

Insert

MAHS

Use

Tags

AcGFP1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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Fragment 1 Null

Plasmid

#198729

Purpose

Gateway-compatible gRNA backbone . Template for the addition of target sequences to produce 2x gRNA.

Depositor

Friedrich Kragler

Article

Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

gRNA expression cassette in pENTR4

Use

Gateway-compatible entry vector

Tags

Expression

Mutation

Promoter

Available since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pSCL.139

Plasmid

#184994

Purpose

Express Cas9-P2A-Eco1RT in human cells

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

SpCas9-P2A-Eco1RT

Use

Tags

SV40NLS

Expression

Mammalian

Mutation

Human codon optimized RT

Promoter

TetOn-3g

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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Osa-pCAMBIA-1301-STTM319

Plasmid

#84201

Purpose

Disturb the expression of miR319 in rice

Depositor

Guiliang Tang

Article

Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

Insert

miR319

Use

Tags

Expression

Bacterial, Plant, and Yeast…

Mutation

Promoter

2x35S

Available since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pMTTH+eGFP-bromo5

Plasmid

#157804

Purpose

Expression of synthetic protein composed of eGFP and 5 repeats of BRD4 bromodomain #1

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

eGFP-bromo5

Use

Tags

His6 and MBP

Expression

Bacterial

Mutation

Promoter

pTAC

Available since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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Gma-pCAMBIA-1301-STTM171

Plasmid

#84080

Purpose

Disturb the expression of miR171 in soybean

Depositor

Guiliang Tang

Article

Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

Insert

miR171

Use

Tags

Expression

Bacterial, Plant, and Yeast…

Mutation

Promoter

2x35S

Available since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pMTTH+eGFP-bromo1

Plasmid

#157805

Purpose

Expression of synthetic protein composed of eGFP and BRD4 bromodomain #1

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

eGFP-bromo1

Use

Tags

His6 and MBP

Expression

Bacterial

Mutation

Promoter

pTAC

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pGENE:EFF-1::mCherry::V5

Plasmid

#133020

Purpose

EFF-1 fused to mCherry fluorescent protein cloned into the pGene vector for inducible expression in mammalian cells

Depositor

Benjamin Podbilewicz

Article

Perez-Vargas et al Cell. 2014 Apr 10;157(2):407-419. doi: 1

Insert

EFF-1::mCherry::V5

Use

Tags

V5; mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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Grp1-PH pET11a

Plasmid

#71377

Purpose

Phosphoinositide Binding

Depositor

Mark Lemmon

Article

Ferguson et al Mol Cell. 2000 Aug;6(2):373-84.

Insert

Grp1-PH (Cyth3 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Pleckstrin homology (PH) domain; aa 264-391

Promoter

T7

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only
pAcGFP1-N1-RvLEAM

Plasmid

#90035

Purpose

To express GFP-tagged RvLEAM in mammalian cells transiently

Depositor

Takekazu Kunieda

Article

Tanaka et al PLoS One. 2015 Feb 12;10(2):e0118272. eC

Insert

RvLEAM

Use

Tags

AcGFP1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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hBACCS2

Plasmid

#67625

Purpose

Mammalian expression of hBACCS2, a human blue light-activated Ca2+ channel switch

Depositor

Takao Nakata

Article

Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

Insert

hBACCS2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pES.HSV2.3a

Plasmid

#69593

Purpose

TCR a chain construct for generation of gBT-I mice

Depositor

Francis R. Carbone

Article

Mueller et al Immunol Cell Biol. 2002 Apr;80(2):156-63

Insert

TCR alpha chain cDNA from clone HSV2.3 (Tcra Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

H-2Kb

Available since

Oct. 7, 2015

Availability

Academic Institutions and Nonprofits only
pHW-CIBN-MP-HRW-CRY2-VHH(GFP)

Plasmid

#126024

Purpose

express RFP-tagged LARIAT for optogenetic clustering

Depositor

Eurico Morais-de-Sá

Article

Osswald et al Biomolecules. 2019 Feb 12;9(2). pii: bio

Insert

CIBN-MP; CRY2 PHR-VHH(GFP)

Use

Tags

RFP

Expression

Insect

Mutation

WT

Promoter

Hsp70

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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p3A9cbetaTCR.HSV2.3

Plasmid

#69594

Purpose

TCR beta chain genomic transgenic construct for generation of gBT-I mice

Depositor

Francis R. Carbone

Article

Mueller et al Immunol Cell Biol. 2002 Apr;80(2):156-63

Insert

TCR beta VJ region from clone HSV2.3 (Tcrb Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only
pHW-CIBN-MP-HFW-CRY2-VHH(GFP)

Plasmid

#126027

Purpose

express Flag-tagged LARIAT for optogenetic clustering

Depositor

Eurico Morais-de-Sá

Article

Osswald et al Biomolecules. 2019 Feb 12;9(2). pii: bio

Insert

CIBN-MP; CRY2 PHR-VHH(GFP)

Use

Tags

Flag

Expression

Insect

Mutation

WT

Promoter

Hsp70

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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