We narrowed to 9,142 results for: App

1. pET28b-His-hCE

   Plasmid

   #112710

   Purpose

   Expresses N-terminally His-tagged human CE/RNGTT in bacterial cells

   Depositor

   Daniel Schoenberg

   Article

   Trotman et al Nucleic Acids Res. 2017 Oct 13;45(18):10

   Insert

   RNGTT (RNGTT Human)
   

   Tags

   6xHis and Stop codons placed after RNGTT sequence…

   Expression

   Bacterial

   Mutation

   R594H

   Promoter

   T7

   Available Since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGMCtKq28-TSC10M1-sspB

   Plasmid

   #49526

   Purpose

   Kanamycin resistant; contains qtag28, expresses sspB under a promoter containing PtetO, and constutively expresses single-chain TetR10 (tsc10); integrates into the mycobacteriophage att-Tweety site

   Depositor

   Dirk Schnappinger

   Article

   Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

   Insert

   TSC10M1-sspB
   

   Expression

   Bacterial

   Available Since

   Jan. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGMCtN-TSC10M1-sspB

   Plasmid

   #49521

   Purpose

   Nourseothricin resistant; expresses sspB under the control of a promoter containing PtetO and constitutively expressses single-chain TetR10 (tsc10); integrates into mycobacteriophage att-Tweety site

   Depositor

   Dirk Schnappinger

   Article

   Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

   Insert

   TSC10M1-sspB
   

   Available Since

   Jan. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-myc NES-mCE NLS

   Plasmid

   #82466

   Purpose

   Expresses cytoplasmic restricted form of myc tagged mRNA capping enzyme

   Depositor

   Daniel Schoenberg

   Article

   Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

   Insert

   NES mRNA capping enzyme (Rngtt HIV, Mouse)
   

   Tags

   Myc

   Expression

   Mammalian

   Available Since

   Sept. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJEC726

   Plasmid

   #190814

   Purpose

   CRISPRi backbone plasmid harboring BbsI sites for easy cloning of sgRNA targeting regions via Golden gate.

   Depositor

   James Chappell

   Article

   Ameruoso et al Nucleic Acids Res. 2022 Jul 22;50(13):77

   Insert

   dCas9
   

   Use

   CRISPR and Synthetic Biology

   Mutation

   S. pyogenes dCas9 codon optimized for Streptomyce…

   Promoter

   SP30

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJEC819

   Plasmid

   #190815

   Purpose

   CRISPRa backbone plasmid harboring BbsI sites for easy cloning of sgRNA targeting regions via Golden gate.

   Depositor

   James Chappell

   Article

   Ameruoso et al Nucleic Acids Res. 2022 Jul 22;50(13):77

   Insert

   dCas9-XTEN-rpoA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   XTEN (linker), followed by the N-terminal domain …

   Promoter

   SP30

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMX-mTAF6

   Plasmid

   #44337

   Depositor

   Marc Timmers

   Article

   Pijnappel et al Nature. 2013 Mar 28;495(7442):516-9. doi

   Insert

   TAF6
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   LTR

   Available Since

   Oct. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. PB-eDIO-EYFP

   Plasmid

   #104388

   Purpose

   PiggyBac plasmid encoding EYFP in an inverse orientation flanked by loxP and lox2722 sites under the control of the EF1a promotor.

   Depositor

   Volker Busskamp

   Article

   Klapper et al Sci Rep. 2017 Oct 31;7(1):14450. doi: 10

   Insert

   Enhanced Yellow Fluorescent Protein
   

   Use

   Cre/Lox; Piggybac

   Expression

   Mammalian

   Available Since

   Jan. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. Sf-U7snRNA-ESE-AS-NsiI

   Plasmid

   #190694

   Purpose

   Basic vector used for the expression of U7-based antisense oligonucleotides

   Depositor

   Pim Pijnappel

   Article

   van der Wal et al Mol Ther Nucleic Acids. 2017 Jun 16;7:90

   Insert

   U7 snRNA
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   U7 promoter

   Available Since

   April 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG--uMASS_LF

    Plasmid

    #209763

    Purpose

    Expression of uMASS_LF (loss-of-function) in mammalian cells under CAG promoter

    Depositor

    Andre Berndt

    Article

    Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

    Insert

    uMASS_LF
    

    Expression

    Mammalian

    Available Since

    Nov. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-uMASS_1

    Plasmid

    #209762

    Purpose

    Expression of uMASS_1 in mammalian cells under CAG promoter

    Depositor

    Andre Berndt

    Article

    Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

    Insert

    uMASS_1
    

    Expression

    Mammalian

    Available Since

    Nov. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMX-hTAF7

    Plasmid

    #44338

    Depositor

    Marc Timmers

    Article

    Pijnappel et al Nature. 2013 Mar 28;495(7442):516-9. doi

    Insert

    TAF7 (TAF7 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    May 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMX-hTAF10

    Plasmid

    #44340

    Depositor

    Marc Timmers

    Article

    Pijnappel et al Nature. 2013 Mar 28;495(7442):516-9. doi

    Insert

    TAF10 (TAF10 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    July 19, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pETM33_PEX14_Pex14

    Plasmid

    #178484

    Purpose

    Bacterial expression of human domain with His-tag and GST tag

    Depositor

    Ylva Ivarsson

    Article

    Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

    Insert

    PEX14_Pex14 (PEX14 Human, Synthetic)
    

    Tags

    His-GST

    Expression

    Bacterial

    Promoter

    T7/LacO

    Available Since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEX-6p-hDcp2

    Plasmid

    #72216

    Purpose

    bacterial expression of GST-tagged human Dcp2

    Depositor

    Megerditch Kiledjian

    Article

    Wang et al Proc Natl Acad Sci U S A. 2002 Oct 1;99(

    Insert

    Dcp2 (DCP2 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    F146Y and E198K (please see depositor's comm…

    Promoter

    tac

    Available Since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pEN41A-T38S38

    Plasmid

    #49524

    Purpose

    Ampicillin resistant; entry clone encoding reverse TetR (tetR38) and contains attL4 and attL1 sites for recombination into a Gateway destination vector at the attR4 and attR1 sites

    Depositor

    Dirk Schnappinger

    Article

    Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

    Insert

    constitutive TetR38
    

    Promoter

    Ptb38

    Available Since

    Jan. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEN12A-P750

    Plasmid

    #49525

    Purpose

    Ampicillin resistant; entry clone containing PtetO-4C5G and attL1 and attL2 sites for recombination into a Gateway destination vector at the attR4 and attR1 sites

    Depositor

    Dirk Schnappinger

    Article

    Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

    Insert

    P750
    

    Promoter

    P750

    Available Since

    Jan. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMX-mTAF12

    Plasmid

    #44342

    Depositor

    Marc Timmers

    Article

    Pijnappel et al Nature. 2013 Mar 28;495(7442):516-9. doi

    Insert

    TAF12 (Taf12 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    May 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDNA3-Flag-hDcp2

    Plasmid

    #72213

    Purpose

    mammalian expression of Flag-tagged human Dcp2

    Depositor

    Megerditch Kiledjian

    Article

    Wang et al Proc Natl Acad Sci U S A. 2002 Oct 1;99(

    Insert

    Dcp2 (DCP2 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Q170R (please see depositor's comment below)

    Promoter

    CMV

    Available Since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-dMASS_1

    Plasmid

    #209764

    Purpose

    Expression of dMASS_1 in mammalian cells under CMV promoter

    Depositor

    Andre Berndt

    Article

    Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

    Insert

    dMASS_1
    

    Expression

    Mammalian

    Available Since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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