Addgene

We narrowed to 1,682 results for: GAD

Showing: 1241 - 1260 of 1682 results

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pKDC.118

Plasmid

#227188

Purpose

T7 IPTG-driven Cas13a

Depositor

Seth Shipman

Article

Fishman et al Nat Biotechnol. 2024 Sep 5. doi: 10.1038

Insert

LbuCas13a

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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PtetA with pBELO_L6-PT3-T4_CAMR_TAN+

Plasmid

#225644

Purpose

tetA promoter expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistance

Depositor

Andre Sanches Ribeiro

Article

Chauhan et al Biochemistry. 2024 Jul 12. doi: 10.1021/

Insert

tetR/tetA

Use

Tags

mCherry

Expression

Bacterial

Mutation

Promoter

tetA promoter

Available since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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PLac0301 with pBELO_L6-PT3-T4_CAMR_TAN+

Plasmid

#225643

Purpose

Lac0301 promoter expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistance

Depositor

Andre Sanches Ribeiro

Article

Chauhan et al Biochemistry. 2024 Jul 12. doi: 10.1021/

Insert

LacO3O1

Use

Tags

mCherry

Expression

Bacterial

Mutation

Promoter

LacO3O1 promoter

Available since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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ParaBAD with pBELO_L6-PT3-T4_CAMR_TAN+

Plasmid

#225642

Purpose

araBAD promoter expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistance

Depositor

Andre Sanches Ribeiro

Article

Chauhan et al Biochemistry. 2024 Jul 12. doi: 10.1021/

Insert

araBAD

Use

Tags

mCherry

Expression

Bacterial

Mutation

Promoter

BAD promoter

Available since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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MGS0156_pET29b

Plasmid

#215410

Purpose

Expression construct for MGS0156 gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

MGS0156

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCSJ182

Plasmid

#159723

Purpose

Express Gid4 (YBR105C) in yeast two hybrid plasmid, pGADCg

Depositor

Alexander Varshavsky

Article

Chen et al Science. 2017 Jan 27;355(6323). pii: 355

Insert

GID4 (VID24 Budding Yeast)

Use

Yeast two-hybrid assay

Tags

3xflag and Gal4AD

Expression

Yeast

Mutation

remove stop codon

Promoter

ADH1

Available since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCSJ392

Plasmid

#159722

Purpose

Express Gid10 (YGR066C) in yeast two hybrid plasmid, pGADCg

Depositor

Alexander Varshavsky

Article

Melnykov et al Proc Natl Acad Sci U S A. 2019 Aug 6;116

Insert

GID10 (GID10 Budding Yeast)

Use

Yeast two-hybrid assay

Tags

3xflag and Gal4AD

Expression

Yeast

Mutation

remove stop codon

Promoter

ADH1

Available since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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NylCK-TS_pET28a

Plasmid

#215423

Purpose

Expression construct for NylCK-TS gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

NylCK-TS

Use

Tags

Expression

Bacterial

Mutation

S111G/A137L

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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NylCK_pET28a

Plasmid

#215422

Purpose

Expression construct for NylCK gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

NylCK

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AfNitB_pET29b

Plasmid

#215401

Purpose

Expression construct for AfNitB gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

AfNitB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pRDA_887

Plasmid

#201164

Purpose

Lentiviral expression of EnAsCas12a CRISPRa gRNA with N' nanobody-ALFA and C' p65 and NLS

Depositor

John Doench

Article

Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

Insert

Puromycin resistance

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

EFS

Available since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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NylCA_pET28a

Plasmid

#215418

Purpose

Expression construct for NylCA gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

NylCA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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NylCA-TS_pET28a

Plasmid

#215419

Purpose

Expression construct for NylCA-TS gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

NylCA-TS

Use

Tags

Expression

Bacterial

Mutation

S111G/A137L/E263Q

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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NylCp2_pET28a

Plasmid

#215420

Purpose

Expression construct for NylCp2 gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

NylCp2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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NylCp2-TS_pET28a

Plasmid

#215421

Purpose

Expression construct for NylCp2-TS gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

NylCp2-TS

Use

Tags

Expression

Bacterial

Mutation

D122G/H130Y/D36A/E263Q

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pRDA_886

Plasmid

#201162

Purpose

Lentiviral expression of EnAsCas12a CRISPRa gRNA with N' nanobody-ALFA and C' VP64 and NLS

Depositor

John Doench

Article

Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

Insert

Puromycin resistance

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

EFS

Available since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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NylB'_pET21b

Plasmid

#215416

Purpose

Expression construct for NylB' gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

NylB'

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-mRuby3

Plasmid

#171154

Purpose

Encodes mRuby3 fluorophore

Depositor

Hillel Adesnik

Article

Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

Insert

mRuby3

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pDIV643

Plasmid

#226182

Purpose

Expresses a color marker (uidA) cassette for disruption on KU70 gene in K. phaffii.

Depositor

Uffe Mortensen

Article

Strucko et al FEMS Yeast Res. 2024 Aug 23:foae026. doi

Insert

uidA marker flanked with targeting regions to KU70 gene in K.phaffii

Use

Tags

Expression

Bacterial

Mutation

Promoter

TEF1p

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pRDA_888

Plasmid

#201165

Purpose

Lentiviral expression of EnAsCas12a CRISPRa gRNA with N' nanobody-ALFA and C' p65, HSF1 and NLS

Depositor

John Doench

Article

Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

Insert

Puromycin resistance

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

EFS

Available since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge