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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 10,970 results for: T7

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Showing: 1241 - 1260 of 10970 results

  1. pNIC-CH

    Plasmid
    #26117
    Purpose
    SGC Empty backbone for bacterial expression
    Depositor
    Opher Gileadi
    Type
    Empty backbone
    Tags
    His
    Expression
    Bacterial
    Promoter
    T7-lacO
    Available Since
    Sept. 17, 2010
    Availability
    Academic Institutions and Nonprofits only
    26117-plasmid-map-sequence-id-12262

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  2. pCDB179

    Plasmid
    #91960
    Purpose
    empty expression vector for 10His-SUMO tag protein purification
    Depositor
    Christopher Bahl
    Type
    Empty backbone
    Tags
    10-His and Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Sept. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    91960-plasmid-map-sequence-id-221552

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  3. pAcGFP-stop-IVT

    Plasmid
    #205146
    Purpose
    IVT template of AcGFP stop codon between the ORF and MCS and polyA
    Depositor
    Igor Ulitsky
    Insert
    AcGFP1
    Use
    In-vitro transcription
    Promoter
    T7
    Available Since
    Aug. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    205146-plasmid-map-sequence-id-404879

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  4. pET-72B1

    Plasmid
    #59374
    Purpose
    Inducible expression of 71B2 (Q9M156) under T7 promoter
    Depositor
    Christopher Anderson
    Insert
    UGT72B1
    Expression
    Bacterial
    Available Since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    59374-plasmid-map-sequence-id-92100

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  5. pNRB65

    Plasmid
    #36455
    Depositor
    Douglas Bishop
    Insert
    ARS501-LEU2 (LEU2 Budding Yeast)
    Expression
    Yeast
    Promoter
    T7
    Available Since
    Aug. 29, 2012
    Availability
    Academic Institutions and Nonprofits only
    36455-plasmid-map-sequence-id-45658

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  6. pET28a(+)-8HisG2K

    Plasmid
    #225540
    Purpose
    Empty backbone for bacterial expression. 8xHis tag, K in position 2 to minimise posttranslational gluconoylation.
    Depositor
    Gregory Challis
    Type
    Empty backbone
    Tags
    8xHis
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Nov. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    225540-plasmid-map-sequence-id-493788

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  8. pET28b(+)-HP0377 C92A

    Plasmid
    #39335
    Depositor
    SeWon Suh
    Insert
    oxidoreductase
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    changed Cysteine 92 to Alanine
    Promoter
    T7
    Available Since
    Sept. 17, 2012
    Availability
    Academic Institutions and Nonprofits only

  10. NLuc-His10

    Plasmid
    #234567
    Purpose
    Bacterially expressed NLuc for Ni-NTA purification
    Depositor
    James Inglese
    Article
    Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:
    Insert
    Nano luciferase
    Tags
    T7 gene 10 leader peptide and Thrombin site-10xHi…
    Expression
    Bacterial
    Mutation
    Codon optimized for bacterial expression
    Promoter
    T7
    Available Since
    May 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    234567-plasmid-map-sequence-id-466796

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  11. pETM6-E12-vioABECD

    Plasmid
    #66537
    Purpose
    pETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with optimized promoters (E12)
    Depositor
    Mattheos Koffas
    Article
    Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10
    Inserts
    VioA
    VioB
    VioE
    VioC
    VioD
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    Mutant T7 Promoter - G6 and Mutant T7 Promoter - …
    Available Since
    July 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    66537-plasmid-map-sequence-id-114568

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  13. TDH3pro-rtTA-tetO7pro-T7pol-pRS413

    Plasmid
    #239293
    Purpose
    Expresses the reverse tetracycline activator (rtTA) and T7 polymerase; Plasmid #1 of the tet-on overexpression system
    Depositor
    Anita Hopper
    Article
    Nostramo et al Mol Cell. 2025 Feb 20;85(4):726-741.e6.
    Inserts
    T7 polymerase with NLS and P278L
    reverse tetracycline transactivator (rtTA)
    Expression
    Yeast
    Mutation
    Contains M2-SE-G72P mutations, described in Addge…
    Promoter
    TDH3 and tetracycline-responsive element (TRE) an…
    Available Since
    June 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    239293-plasmid-map-sequence-id-476828

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  20. pSL0375 (His-MBP-TniQ_Cas8_Cas7_Cas6)

    Plasmid
    #130640
    Purpose
    Expresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from a T7 promoter, with N-terminal His10-MBP-TEVsite tag on TniQ for purification.
    Depositor
    Samuel H. Sternberg
    Article
    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:
    Insert
    VchTniQ, VchCas8, VchCas7, VchCas6
    Use
    CRISPR; Transposon
    Tags
    His10-MBP-TEVsite on TniQ
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Sept. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    130640-plasmid-map-sequence-id-255648

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Showing: 1241 - 1260 of 10970 results