We narrowed to 63,044 results for: cloning
-
Plasmid#91049PurposeModule A, Promoter: 35S, Gene: TALE-VP64_1 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: none (ends with P2A to be fused to TALE-VP64_ 2)DepositorInsertTALE-VP64_1 backbone with Esp3I ccdb cassette for repeat cloning
UseTALENPromoter35SAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_B2103b
Plasmid#91062PurposeModule B, Promoter: CmYLCV, Gene: SapI ccdb cassette (doesn't include the promoter) for cloning multiple gRNA spacers with Csy4 spacers , Terminator: 35SDepositorInsertSapI ccdb cassette (no promoter) for cloning multiple gRNA spacers with Csy4 spacers
UseCRISPRPromoterCmYLCVAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hDlx-Flex-GFP-Fishell_6
Plasmid#83895PurposeCre recombinase-dependent GFP expression in forebrain GABA-ergic interneurons under the control of the hDlx enhancer elementDepositorHas serviceAAV1, AAV2, AAV8, AAV9, and AAV RetrogradeInserteGFP
UseAAV and Cre/LoxExpressionMammalianPromoterhDlxAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-S5E2-GFP-fGFP
Plasmid#135631PurposeAAV vector to drive the expression of fGFP in PV cortical interneuronsunder the control of the E2 regulatory elementDepositorHas serviceAAV1 and AAV9InsertGFP-fGFP
UseAAVMutationNoneAvailable sinceAug. 17, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pALuWBB
Plasmid#183202PurposedsRed marked transgene for Crispr co-conversion with w+ gRNA and drop in cloning sites for 2 more gRNADepositorInsertw+ gRNA and scaffold followed by 2 gRNA with BbsI and BsaI cloning sites
UseCRISPR; Recombines at attp landing site in fly ge…ExpressionInsectPromoterU6-3Available sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-S5E2-GCaMP6f
Plasmid#135632PurposeAAV vector to drive the expression of GCaMP6f in PV cortical interneuronsunder the control of the E2 regulatory elementDepositorHas serviceAAV1, AAV9, and AAV PHP.eBInsertGCaMP6f
UseAAVMutationNoneAvailable sinceAug. 17, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hsyn-GRAB_rDA1m
Plasmid#140556PurposeExpresses the red fluorescent medium-affinity DA sensor GRAB_rDA1m in neuronsDepositorHas serviceAAV9InsertRed fluorescent DA sensor GRAB_rDA1m (medium affinity)
UseAAVPromoterhSynAvailable sinceJuly 29, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn-DIO-ChrimsonR-mRuby2-ST
Plasmid#105448PurposeCation channelrhodopsin ChrimsonR fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites in a viral vectorDepositorHas serviceAAV9InsertChrimsonR-mRuby2-ST
UseAAVTagsmRuby2 and soma targeting motifMutationK176R in ChrimsonPromoterhSynAvailable sinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
JM110
Bacterial strain#49763PurposeE.coli K-12 derivativeDepositorPublicationBacterial resistanceStreptomycinAvailable sinceOct. 14, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-CAG-DIO-ChroME-ST-P2A-H2B-mRuby3
Plasmid#108912PurposeCation channelrhodopsin ChroME targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vectorDepositorHas serviceAAV9InsertChroME-ST
UseAAVPromoterCAGAvailable sinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-S5E2-Gq-P2A-dTomato
Plasmid#135635PurposeAAV vector to drive the expression of Gq-DREADD-P2A-dTomato in PV cortical interneuronsunder the control of the E2 regulatory elementDepositorHas serviceAAV1, AAV9, and AAV PHP.eBInsertGq-P2A-dTomato
UseAAVMutationNoneAvailable sinceAug. 17, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-syn-FLEX-splitTVA-EGFP-tTA
Plasmid#100798Purposehelper virus for monosynaptic tracing; to be coinjected with pAAV-TREtight-mTagBFP2-B19GDepositorHas serviceAAV1InsertTVA, EGFP, tet transactivator
UseAAVExpressionMammalianPromotersynapsinAvailable sinceOct. 5, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGP-AAV-syn-iGABASnFR2-WPRE
Plasmid#218874PurposeAAV-mediated expression of improved GABA sensor (positive change in fluorescence)DepositorHas serviceAAV1 and AAV5InsertiGABASnFR2
UseAAVExpressionMammalianMutationS99A F102Y F104Y L178SPromoterSynapsinAvailable sinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV-CamKIIa-jGCaMP8m-WPRE
Plasmid#176751PurposeAAV-mediated expression of GCaMP8m under the CamKIIa promoterDepositorHas serviceAAV1, AAV5, AAV9, and AAV RetrogradeInsertjGCaMP8m
UseAAVTags6xHisExpressionMammalianPromoterCamKIIalphaAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-S5E2-ChR2-mCherry
Plasmid#135634PurposeAAV vector to drive the expression of ChR2-mCherry in PV cortical interneuronsunder the control of the E2 regulatory elementDepositorHas serviceAAV1, AAV9, and AAV PHP.eBInsertChR2-mCherry
UseAAVMutationNoneAvailable sinceAug. 17, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-TREtight-mTagBFP2-B19G
Plasmid#100799Purposehelper virus for monosynaptic tracing; to be coinjected with pAAV-syn-FLEX-splitTVA-EGFP-tTADepositorHas serviceAAV1InsertmTagBFP2 and rabies virus SAD B19 strain glycoprotein genes (B19G)
UseAAVExpressionMammalianPromoterTREtightAvailable sinceOct. 5, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV-CamKIIa-jGCaMP8f-WPRE
Plasmid#176750PurposeAAV-mediated expression of GCaMP8f under the CamKIIa promoterDepositorHas serviceAAV1, AAV5, AAV9, and AAV RetrogradeInsertjGCaMP8f
UseAAVTags6xHisExpressionMammalianPromoterCamKIIalphaAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-CAG-SomaGCaMP6f2
Plasmid#158757PurposeCell body-targeted GCaMP6f, under CAG promoter: GCaMP6f followed by a linker, and the EE-RR coiled coil motif (GCaMP6f-27-EE-RR). As bright as conventional GCaMP6f.DepositorHas serviceAAV8 and AAV9InsertSomaGCaMP6f2
UseAAVExpressionMammalianPromoterCAG promoterAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP
Plasmid#112677PurposeAn AAV vector that expresses a Cre-dependent nuclear-localized Red to Green Fluorescent proteinDepositorHas serviceAAV1, AAV2, AAV5, AAV8, AAV9, and AAV RetrogradeInsertNuclear-localized floxed-mCherry EGFP
UseAAVTagsNuclear localization signalPromoterEF1aAvailable sinceMarch 7, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSynapsin-FLEX-soCoChR-GFP
Plasmid#107712PurposeA somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. Cre-dependent virusDepositorHas serviceAAV9InsertsoCoChR-GFP
UseAAVTagsEGFP and KA2ExpressionMammalianPromoterhSynapsinAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by