We narrowed to 63,783 results for: cloning
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Plasmid#140554PurposeExpresses the 2nd generation high-affinity DA sensor GRAB_DA2h in neuronsDepositorHas serviceAAV9InsertThe 2nd generation DA sensor GRAB_DA2h (high affinity)
UseAAVPromoterhSynAvailable sinceJuly 29, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-CKIIa-stGtACR2-FusionRed
Plasmid#105669PurposeExpresses a soma-targeted GtACR2 in mammalian neurons under the CaMKIIa promoterDepositorHas serviceAAV1 and AAV RetrogradeInsertstGtACR2
UseAAVTagsFusionRed and Kv2.1ExpressionMammalianPromoterCaMKIIaAvailable sinceFeb. 23, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV_hSyn1-SIO-stGtACR2-FusionRed
Plasmid#105677PurposeExpresses a soma-targeted GtACR2 in Cre-expressing neuronsDepositorHas serviceAAV1, AAV5, and AAV RetrogradeInsertstGtACR2
UseAAVTagsFusionRed and Kv2.1ExpressionMammalianPromoterhSyn1Available sinceFeb. 23, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMOD_B3200
Plasmid#91079PurposeModule B, Promoter: none (begins with P2A to be fused to TALE-VPR_1), Gene: TALE-VPR_2 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: HSPDepositorInsertTALE-VPR_2 backbone with Esp3I ccdb cassette for repeat cloning
UseTALENPromoternone (begins with P2A to be fused to TALE-VPR_1)Available sinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_B3400
Plasmid#91080PurposeModule B, Promoter: none (begins with P2A to be fused to TALE-VP64_1), Gene: TALE-VP64_2 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: HSPDepositorInsertTALE-VP64_2 backbone with Esp3I ccdb cassette for repeat cloning
UseTALENPromoternone (begins with P2A to be fused to TALE-VP64_1)Available sinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A3101
Plasmid#91048PurposeModule A, Promoter: 35S, Gene: TALE-VPR_1 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: none (ends with P2A to be fused to TALE-VPR_ 2)DepositorInsertTALE-VPR_1 backbone with Esp3I ccdb cassette for repeat cloning
UseTALENPromoter35SAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A3301
Plasmid#91049PurposeModule A, Promoter: 35S, Gene: TALE-VP64_1 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: none (ends with P2A to be fused to TALE-VP64_ 2)DepositorInsertTALE-VP64_1 backbone with Esp3I ccdb cassette for repeat cloning
UseTALENPromoter35SAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hDlx-Flex-GFP-Fishell_6
Plasmid#83895PurposeCre recombinase-dependent GFP expression in forebrain GABA-ergic interneurons under the control of the hDlx enhancer elementDepositorHas serviceAAV1, AAV2, AAV8, AAV9, and AAV RetrogradeInserteGFP
UseAAV and Cre/LoxExpressionMammalianPromoterhDlxAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-S5E2-GFP-fGFP
Plasmid#135631PurposeAAV vector to drive the expression of fGFP in PV cortical interneuronsunder the control of the E2 regulatory elementDepositorHas serviceAAV1 and AAV9InsertGFP-fGFP
UseAAVMutationNoneAvailable sinceAug. 17, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pALuWBB
Plasmid#183202PurposedsRed marked transgene for Crispr co-conversion with w+ gRNA and drop in cloning sites for 2 more gRNADepositorInsertw+ gRNA and scaffold followed by 2 gRNA with BbsI and BsaI cloning sites
UseCRISPR; Recombines at attp landing site in fly ge…ExpressionInsectPromoterU6-3Available sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-S5E2-GCaMP6f
Plasmid#135632PurposeAAV vector to drive the expression of GCaMP6f in PV cortical interneuronsunder the control of the E2 regulatory elementDepositorHas serviceAAV1, AAV9, and AAV PHP.eBInsertGCaMP6f
UseAAVMutationNoneAvailable sinceAug. 17, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hsyn-GRAB_rDA1m
Plasmid#140556PurposeExpresses the red fluorescent medium-affinity DA sensor GRAB_rDA1m in neuronsDepositorHas serviceAAV9InsertRed fluorescent DA sensor GRAB_rDA1m (medium affinity)
UseAAVPromoterhSynAvailable sinceJuly 29, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn-DIO-ChrimsonR-mRuby2-ST
Plasmid#105448PurposeCation channelrhodopsin ChrimsonR fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites in a viral vectorDepositorHas serviceAAV9InsertChrimsonR-mRuby2-ST
UseAAVTagsmRuby2 and soma targeting motifMutationK176R in ChrimsonPromoterhSynAvailable sinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
JM110
Bacterial strain#49763PurposeE.coli K-12 derivativeDepositorPublicationBacterial resistanceStreptomycinAvailable sinceOct. 14, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-CAG-DIO-ChroME-ST-P2A-H2B-mRuby3
Plasmid#108912PurposeCation channelrhodopsin ChroME targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vectorDepositorHas serviceAAV9InsertChroME-ST
UseAAVPromoterCAGAvailable sinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-S5E2-Gq-P2A-dTomato
Plasmid#135635PurposeAAV vector to drive the expression of Gq-DREADD-P2A-dTomato in PV cortical interneuronsunder the control of the E2 regulatory elementDepositorHas serviceAAV1, AAV9, and AAV PHP.eBInsertGq-P2A-dTomato
UseAAVMutationNoneAvailable sinceAug. 17, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGP-AAV-syn-iGABASnFR2-WPRE
Plasmid#218874PurposeAAV-mediated expression of improved GABA sensor (positive change in fluorescence)DepositorHas serviceAAV1 and AAV5InsertiGABASnFR2
UseAAVExpressionMammalianMutationS99A F102Y F104Y L178SPromoterSynapsinAvailable sinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV-CamKIIa-jGCaMP8m-WPRE
Plasmid#176751PurposeAAV-mediated expression of GCaMP8m under the CamKIIa promoterDepositorHas serviceAAV1, AAV5, AAV9, and AAV RetrogradeInsertjGCaMP8m
UseAAVTags6xHisExpressionMammalianPromoterCamKIIalphaAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-S5E2-ChR2-mCherry
Plasmid#135634PurposeAAV vector to drive the expression of ChR2-mCherry in PV cortical interneuronsunder the control of the E2 regulatory elementDepositorHas serviceAAV1, AAV9, and AAV PHP.eBInsertChR2-mCherry
UseAAVMutationNoneAvailable sinceAug. 17, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-syn-FLEX-splitTVA-EGFP-tTA
Plasmid#100798Purposehelper virus for monosynaptic tracing; to be coinjected with pAAV-TREtight-mTagBFP2-B19GDepositorHas serviceAAV1InsertTVA, EGFP, tet transactivator
UseAAVExpressionMammalianPromotersynapsinAvailable sinceOct. 5, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by