We narrowed to 2,567 results for: gem

1. pCas55

   Plasmid

   #82393

   Purpose

   sgRNA targeting YFP +172 from TSS; non-template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

   Depositor

   Brian Pfleger

   Article

   Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

   Insert

   gRNA
   

   Promoter

   PEZ3

   Available Since

   Sept. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCas52

   Plasmid

   #82392

   Purpose

   sgRNA targeting YFP +52 from TSS; non-template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

   Depositor

   Brian Pfleger

   Article

   Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

   Insert

   gRNA
   

   Promoter

   PEZ3

   Available Since

   Sept. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNP3254 [pNP2922 - ISCro4 Enhanced DBL (DBL3, WT) + ISCro4 Recombianse (WT]

   Plasmid

   #247257

   Purpose

   Express ISCro4 recombinase and bridge RNA

   Depositor

   Patrick Hsu

   Article

   Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1

   Insert

   pNP2922 - ISCro4 bRNA101-179 ins179(rc(101-111) (DBL3)
   

   Expression

   Mammalian

   Mutation

   ins179(rc(101-111)

   Available Since

   Oct. 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNP3414 [pNP2922 - ISCro4 Enhanced TBL (TBL4, WT) + ISCro4 Recombinase (WT)]

   Plasmid

   #247261

   Purpose

   Express ISCro4 recombinase and bridge RNA

   Depositor

   Patrick Hsu

   Article

   Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1

   Insert

   pNP2922 - ISCro4 bRNA1-100 C60U, A61G, U73C, dU86, A87G, dU88 (TBL4)
   

   Expression

   Mammalian

   Mutation

   C60U, A61G, U73C, dU86, A87G, dU88

   Available Since

   Oct. 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX333

   Plasmid

   #64073

   Purpose

   Vector for tandem expression of two sgRNAs from two independent U6 promoters. Cas9 is expressed by Cbh promoter.

   Depositor

   Andrea Ventura

   Article

   Maddalo et al Nature. 2014 Dec 18;516(7531):423-7. doi

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Tags

   3XFLAG-Cas9

   Expression

   Mammalian

   Promoter

   CBh; U6

   Available Since

   May 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSEVA331Bb

   Plasmid

   #78269

   Purpose

   pSEVA331 with Biobrick multiple cloning site

   Depositor

   Tom Ellis

   Article

   Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

   Type

   Empty backbone

   Available Since

   Nov. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. H2A-mScarletI-P2A-Akt-KTR-mTurquoise2-P2A-ERK-KTR-mNeonGreen

   Plasmid

   #129631

   Purpose

   Visualization of Akt and ERK activity

   Depositor

   Dorus Gadella

   Article

   Chavez-Abiega et al J Cell Sci. 2022 Mar 15;135(6):jcs259685

   Insert

   H2A-mScarletI-P2A-Akt-KTR-mTurquoise2-P2A-ERK-KTR-mNeonGreen
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFBD-Akt1(D323A/D325A)

   Plasmid

   #86592

   Purpose

   Expresses human full-length Akt1(D323A/D325A) mutant

   Depositor

   Ivan Yudushkin, Thomas Leonard

   Article

   Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

   Insert

   Akt1 (AKT1 Human)
   

   Tags

   10xHis

   Expression

   Insect

   Mutation

   D323A/D325A transforming mutations

   Available Since

   March 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. p221a-GUS

   Plasmid

   #71263

   Purpose

   Entry clone containing the GUS enzyme. Can be used to construct transcriptional reporters. For use in plants and compatible with the MultiSite Gateway system

   Depositor

   Ari Pekka Mähönen

   Article

   Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

   Insert

   Beta-glucuronidase
   

   Use

   Gateway

   Available Since

   Jan. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1(+)-HA-Flag-natT1R2

    Plasmid

    #113944

    Purpose

    mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin

    Depositor

    Erik Procko

    Article

    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

    Insert

    T1R2 (TAS1R2 Human)
    

    Tags

    FLAG and Signal/leader sequence from influenza he…

    Expression

    Mammalian

    Mutation

    residues 22-839

    Available Since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLIB-HIS-TEV-Cyclin-B

    Plasmid

    #177012

    Purpose

    Generate baculovirus for insect cell expression of Cyclin-B with N-terminal Polyhistidine-TEV

    Depositor

    Andrea Musacchio

    Article

    Huis In 't Veld et al Protein Sci. 2021 Nov 17. doi: 10.1002/p

    Insert

    Cyclin B1 (CCNB1 Human)
    

    Tags

    Polyhistidine-TEV

    Expression

    Insect

    Mutation

    codon-optimised for insect cell expression

    Available Since

    Nov. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. hMLKL-Venus

    Plasmid

    #106078

    Purpose

    expression in mammalian cells

    Depositor

    Douglas Green

    Article

    Quarato et al Mol Cell. 2016 Feb 18;61(4):589-601. doi

    Insert

    human MLKL-Venus (MLKL Human)
    

    Use

    Retroviral; Tet-on retrovirus

    Tags

    Venus

    Expression

    Mammalian

    Promoter

    Dox-inducible

    Available Since

    March 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAV CMV-dSaCas9-KRAB-bGHpA

    Plasmid

    #106219

    Purpose

    AAV expressing deactivated S. aureus Cas9 fused to a KRAB repressor

    Depositor

    Charles Gersbach

    Article

    Thakore et al Nat Commun. 2018 Apr 26;9(1):1674. doi:

    Insert

    dead S. aureus Cas9 KRAB
    

    Use

    AAV and CRISPR

    Tags

    HA-NLS

    Expression

    Mammalian

    Mutation

    D10A and N580A

    Promoter

    CMV

    Available Since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. PMP34-mCherry-EGFP-Tet-On

    Plasmid

    #109017

    Purpose

    Tet-On construct to induce expression of tandem mCherry-eGFP tagged PMP34 (peroxisome marker)

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    PMP34 (SLC25A17 Human)
    

    Use

    Lentiviral

    Tags

    mCherry-eGFP

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET-scFv-T

    Plasmid

    #67843

    Purpose

    pET-30-based vector dedicated to efficient scFv expression, which circumvents the problem of in-frame amber codons in scFvs.

    Depositor

    Joanna Bereta

    Article

    Ossysek et al Immunol Lett. 2015 Jul 25. pii: S0165-24

    Insert

    antibody 1D8 light kappa chain
    

    Tags

    His-tag

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGW03 AAV-TBG-Cre-sgRNA

    Plasmid

    #192163

    Purpose

    AAV-TBG-Cre-sgRNA

    Depositor

    Sidi Chen

    Article

    Wang et al Cancer Discov. 2020 Dec;10(12):1912-1933

    Type

    Empty backbone

    Use

    AAV

    Mutation

    NA

    Available Since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. TOM20MTS-mCherry-EGFP-Tet-On

    Plasmid

    #109016

    Purpose

    Tet-On construct to induce expression of tandem eGFP-mCherry tagged TOM20MTS (mitochondria

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    TOM20MTS (MTS: mitochondria targeting signal) (TOMM20 Human)
    

    Use

    Lentiviral

    Tags

    mCherry-eGFP

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA 3.1 AnxA6

    Plasmid

    #20407

    Depositor

    Carlos Enrich

    Article

    Vila de Muga et al Oncogene. 2009 Jan 22. 28(3):363-77.

    Insert

    AnxA6 (Anxa6 Rat)
    

    Expression

    Mammalian

    Available Since

    May 1, 2009

    Availability

    Academic Institutions and Nonprofits only

19. Adeno EA

    Plasmid

    #64071

    Purpose

    Adenovirus for the expression of gRNAs targeting intron 19 of murine Alk and intron 14 of Eml4

    Depositor

    Andrea Ventura

    Article

    Maddalo et al Nature. 2014 Dec 18;516(7531):423-7. doi

    Insert

    U6_sgRNA(Alk)_U6_sgRNA(Eml4)_CBh_FLAG-Cas9
    

    Use

    Adenoviral

    Tags

    FLAG-Cas9

    Promoter

    U6 and CBh

    Available Since

    July 23, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pLenti-XI-Neo-GST-Constitutively Active AMPK

    Plasmid

    #139843

    Purpose

    Lentiviral expression of GST-tagged Constitutively Active rat AMPK

    Depositor

    Jacob Corn

    Article

    Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

    Insert

    AMPK (Prkaa2 Rat)
    

    Use

    Lentiviral

    Tags

    GST

    Available Since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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