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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 22,329 results for: nes

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Showing: 1241 - 1260 of 22329 results

  1. pcDNA3.1_pCMV-nCas-PmCDA1-ugi pH1-gRNA(HPRT)

    Plasmid
    #79620
    Purpose
    Expresses nCas9-PmCDA1-UGI and gRNA(HPRT) in mammalian cells
    Depositor
    Akihiko Kondo
    Publication
    Nishida et al Science. 2016 Aug 4. pii: aaf8729.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9
    Tags
    PmCDA1 and UGI
    Expression
    Mammalian
    Mutation
    D10A for nickase Cas9
    Promoter
    pCMV
    Available since
    July 27, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    16 Articles
    79620-plasmid-map-sequence-id-154120

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  9. MSP2443

    Plasmid
    #72252
    Purpose
    Human expression plasmid for SpCas9-VRQR-HF1 variant: CMV-T7-humanSpCas9-VRQR-HF1(N497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335Q, T1337R)-NLS-3xFLAG
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 VRQR-HF1(N497A/R661A/Q695A/Q926A/D1135V/G1218R/R1335Q/T1337R)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335…
    Promoter
    CMV
    Available since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72252-plasmid-map-sequence-id-207329

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  13. TtRMPVIR

    Plasmid
    #27995
    Purpose
    Retroviral transfer plasmid for Tet-regulated shRNA expression with a TREtight promoter. Contains DsRed2 and Venus fluorescent reporters.
    Depositor
    Scott Lowe
    Publication
    Zuber et al Nat Biotechnol. 2011 Jan . 29(1):79-83.
    Insert
    TtRMPVIR (shRen)
    Use
    RNAi and Retroviral; Fluorescent reporters
    Expression
    Mammalian
    Available since
    March 18, 2011
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    7 Articles
    27995-plasmid-map-sequence-id-13250

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  14. lentiSAM v2 (Puro)

    Plasmid
    #92062
    Purpose
    Modified version of lentiSAM v2, a lenti sgRNA cloning backbone with MS2 loops at tetraloop/stemloop 2, dCas9-VP64, and puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Adam Karpf
    Publication
    Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6 (sgRNA) and EF1a (dCas9-VP64)
    Available since
    May 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    13 Articles
    92062-plasmid-map-sequence-id-180159

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Showing: 1241 - 1260 of 22329 results