161,271 results

1. pCMV-PE6a

   Plasmid

   #207851

   Purpose

   Mammalian expression of PE6a prime editor

   Depositor

   David Liu

   Article

   Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

   Insert

   PE6a
   

   Use

   Tags

   SV40 bpNLS and SV40 bpNLS, c-Myc NLS

   Expression

   Mammalian

   Mutation

   Ec48rt(E60K, K87E, E165D, D243N, R267I, E279K, K3…

   Promoter

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGL3-TK-5UTR-BsmBI-Luciferase

   Plasmid

   #114670

   Purpose

   For one-step cloning (with blue/ white screening) of a desired 5' UTR to test its impact on translation of a firefly luciferase reporter

   Depositor

   Lynne Postovit

   Article

   Jewer et al Nat Commun. 2020 May 19;11(1):2498. doi:

   Insert

   LacZ
   

   Use

   Luciferase

   Tags

   for cloning 5' UTR-Luciferase fusions

   Expression

   Mammalian

   Mutation

   Promoter

   lac promoter

   Available since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. tdTomato-Keratin-17

   Plasmid

   #58106

   Purpose

   Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 554, Emission: 581

   Depositor

   Michael Davidson

   Insert

   Keratin (KRT18 Human)
   

   Use

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   V282L

   Promoter

   CMV

   Available since

   Dec. 23, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pACU2_CD4-mIFP T2A HO1

   Plasmid

   #72441

   Purpose

   Expressing UAS-CD4-mIFP T2A HO1 in drosophila

   Depositor

   Xiaokun Shu

   Article

   Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

   Insert

   CD4-mIFP T2A HO1
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 29, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pQE-60 Grx1-roGFP2-His

   Plasmid

   #64799

   Purpose

   Bacterial expression of His-tagged Grx1-roGFP2

   Depositor

   Tobias Dick

   Article

   Gutscher et al Nat Methods. 2008 Jun;5(6):553-9. doi: 1

   Insert

   Glutaredoxin-1 (GLRX Human)
   

   Use

   Tags

   His and roGFP2

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET22b_PstS_3

   Plasmid

   #78200

   Purpose

   Expression of double-cysteine variant of mature phosphate binding protein, PstS, which also has a mutation to weaken phosphate binding, as scaffold for phosphate biosensor

   Depositor

   Martin Webb

   Article

   Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62

   Insert

   pstS (A17C,I76G,A197C)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   A17C,I76G,A197C (please see depositor comment bel…

   Promoter

   T7

   Available since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-hSyn-mScarlet (AAV1)

   Viral Prep

   #131001-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pAAV-hSyn-mScarlet (#131001). In addition to the viral particles, you will also receive purified pAAV-hSyn-mScarlet plasmid DNA. Synapsin-driven mScarlet control vector. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Karl Deisseroth

   Article

   Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

   Promoter

   Syn

   Tags

   mScarlet

   Available since

   Nov. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

8. pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA (AAV8)

   Viral Prep

   #20297-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA (#20297). In addition to the viral particles, you will also receive purified pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA plasmid DNA. Humanized channelrhodopsin H134R mutant fused to mCherry, driven by the EF1a promoter. Cre-dependent. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Karl Deisseroth

   Promoter

   EF1a

   Tags

   mCherry (Cre-dependent)

   Available since

   Dec. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

9. pGilesSHdI_redgreen_2395A_His6_triple red_pLeft1806_His6_double green

   Plasmid

   #170048

   Purpose

   Tri-Responsive Reporter Gene Construct for redgreen, triple red, and double green

   Depositor

   Clifton Barry III

   Article

   Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

   Inserts

   mScarlet
   COmScarlet
   mRuby3
   mWasabi dN
   mWasabi CO
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPB-cT3G-cERP2-YBX1

    Plasmid

    #192904

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of YBX1

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    YBX1 (YBX1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    TET3G-miniCMV

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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