We narrowed to 6,128 results for: nls
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Plasmid#177281Purposegenomic integration of genes (inserted in I-SceI-site) via transposon Tn5 with TcRDepositorInsertPtet-tetA(C)
UseSynthetic Biology; Yeast expression, tn5 genomic …TagsExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYTRW11K_0S5
Plasmid#177282Purposegenomic integration of genes (inserted in I-SceI-site) via transposon Tn5 with SmRDepositorInsertPaadA-aadA
UseSynthetic Biology; Yeast expression, tn5 genomic …TagsExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYTRW14K_7G5
Plasmid#177283Purposegenomic integration of genes (inserted in I-SceI-site) via transposon Tn5 with GmR and LacZDepositorInsertlacZ
UseSynthetic Biology; Yeast expression, tn5 genomic …TagsExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYTRW15K_2G5
Plasmid#177284Purposegenomic integration of genes (inserted in I-SceI-site) via transposon Tn5 with GmR and mTagBFP2DepositorInsertmTagBFP2
UseSynthetic Biology; Yeast expression, tn5 genomic …TagsExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYTRW17K_6G5
Plasmid#177285Purposegenomic integration of genes (inserted in I-SceI-site) via transposon Tn5 with GmR and PE-HDepositorInsertpe-h
UseSynthetic Biology; Yeast expression, tn5 genomic …TagsExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYTRW16K_1G5
Plasmid#177286Purposegenomic integration of genes (inserted in I-SceI-site) via transposon Tn5 with GmR and eYFPDepositorInserteYFP
UseSynthetic Biology; Yeast expression, tn5 genomic …TagsExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYTRW13K_3G5
Plasmid#177287Purposegenomic integration of genes (inserted in I-SceI-site) via transposon Tn5 with GmR and mCherryDepositorInsertmCherry
UseSynthetic Biology; Yeast expression, tn5 genomic …TagsExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYTRW18K_3T5
Plasmid#177288Purposegenomic integration of genes (inserted in I-SceI-site) via transposon Tn5 with TcR and mCherryDepositorInsertmCherry
UseSynthetic Biology; Yeast expression, tn5 genomic …TagsExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYTRW20K_0Ti1
Plasmid#177289Purposegenomic integration of genes (inserted in I-SceI-site) via rrn-interposon with TcRDepositorInsertPtet-tetA(C)
UseSynthetic Biology; Yeast expression, rrn genomic …TagsExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYTRW28K_0Ti1
Plasmid#177290Purposegenomic integration of genes (inserted in I-SceI-site) via rrn-interposon/SacB with TcRDepositorInsertPsacB-sacB
UseSynthetic Biology; Yeast expression, rrn genomic …TagsExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
iPEmaxΔR-D10A
Plasmid#239381PurposeFor inverse prime editor using nCas9-D10A-R221K-N394K and M-MLV-RTΔRNase H in HEK293T cellsDepositorInsertnCas9(D10A-R221K-N394K), M-MLV-RT(ΔRNase H)
UseCRISPRTagsBPNLS and SV40 NLS, c-myc NLSExpressionMammalianMutationD10A, R221K, N394K in Cas9; ΔRNase H in M-MLV RTPromoterCMVAvailable sinceAug. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
TOPO-SV40
Plasmid#226453PurposeFor subcloning of SV40 promoter or for assays using M13 phageDepositorInsertSV40
UseCloning vectorTagsExpressionMutationNoPromoterAvailable sinceJuly 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
FUS LC TtoS
Plasmid#226608PurposeAll threonines to serineDepositorInsertFUS LC 4QQ->GG (FUS Human)
UseTags6HisExpressionBacterialMutation4 QQ pairs replaced with GG pairsPromoterT7Available sinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
FUS LC QtoA
Plasmid#226609PurposeAll glutamines to alanineDepositorInsert6*His-TEV-FUS LC QtoA (FUS Human)
UseTags6HisExpressionBacterialMutationAll glutamine to alaninePromoterT7Available sinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
FUS LC-RGG1 StoG
Plasmid#226610PurposeAll serines in the LC domain to glycineDepositorInsertFUS LC-RGG1 StoG (FUS Human)
UseTags6HisExpressionBacterialMutationAll serines to glycine in the LC domainPromoterT7Available sinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
FUS LC-RGG1 TtoS
Plasmid#226611PurposeAll threonines in the LC domain to serineDepositorInsertFUS LC-RGG1 TtoS (FUS Human)
UseTags6HisExpressionBacterialMutationAll threonine to serine in the LC domainPromoterT7Available sinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
FUS LC-RGG1 QtoA
Plasmid#226612PurposeAll glutamines in the LC domain to alanineDepositorInsertFUS LC-RGG1 QtoA (FUS Human)
UseTags6HisExpressionBacterialMutationAll glutamine to alanine in the LC domainPromoterT7Available sinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
FUS LC-RGG1 TtoV
Plasmid#226613PurposeAll threonines in the LC domain to valineDepositorInsertFUS LC-RGG1 TtoV (FUS Human)
UseTags6HisExpressionBacterialMutationAll threonine to valine in the LC domainPromoterT7Available sinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
FUS LC-RGG1 QtoG
Plasmid#226614PurposeAll glutamines in the LC domain to glycineDepositorInsertFUS LC-RGG1 QtoG (FUS Human)
UseTags6HisExpressionBacterialMutationAll glutamine to glycine in the LC domainPromoterT7Available sinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
FUS LC-RGG1 QtoN
Plasmid#226615PurposeAll glutamines in the LC domain to asparagineDepositorInsertFUS LC-RGG1 QtoN (FUS Human)
UseTags6HisExpressionBacterialMutationAll glutamine to asparagine in the LC domainPromoterT7Available sinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
FUS LC-RGG1 QtoS
Plasmid#226616PurposeAll glutamines in the LC domain to serineDepositorInsertFUS LC-RGG1 QtoS (FUS Human)
UseTags6HisExpressionBacterialMutationAll glutamine to serine in the LC domainPromoterT7Available sinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
FUS LC noYQ_noYT
Plasmid#226617PurposeT7S / Q160S double mutationDepositorInsertFUS LC noYQ_noYT (FUS Human)
UseTags6HisExpressionBacterialMutationT7S / Q160S double mutation in LC domain to remov…PromoterT7Available sinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
FUS LC-RGG1 wild type
Plasmid#226596PurposeWild type sequence of FUS LC-RGG1 domainsDepositorInsertFUS LC-RGG1 WT (FUS Human)
UseTags6HisExpressionBacterialMutationwild type LC-RGG1PromoterT7Available sinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
FUS LC 12S->N
Plasmid#226599Purpose12 serines to asparaginesDepositorInsertFUS LC 12S->N (FUS Human)
UseTags6HisExpressionBacterialMutation12 serines to asparaginePromoterT7Available sinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
FUS LC 4S->A#1
Plasmid#226603Purpose4 serines to alanine at residue positions 30, 44, 48, 53DepositorInsertFUS LC S->A#1 (FUS Human)
UseTags6HisExpressionBacterialMutation4 serines to alanine in sequence position subset …PromoterT7Available sinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
FUS LC 4S->A#2
Plasmid#226604Purpose4 serines to alanine at residue positions 70, 84, 89, 95DepositorInsertFUS LC 4S->A#2 (FUS Human)
UseTags6HisExpressionBacterialMutation4 serines to alanine in sequence position subset …PromoterT7Available sinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
FUS LC 4S->A#3
Plasmid#226605Purpose4 serines to alanine at residue positions 108, 117, 119, 127DepositorInsertFUS LC 4S->A#3 (FUS Human)
UseTags6HisExpressionBacterialMutation4 serines to alanine in sequence position subset …PromoterT7Available sinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
FUS LC 8Y->S
Plasmid#226606Purpose8 tyrosines to serineDepositorInsertFUS LC 8Y->S (FUS Human)
UseTags6HisExpressionBacterialMutation8 tyrosines to serinePromoterT7Available sinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
p70.unipA.NGFR.mCMV.PGK.mBFP2.p27K.WPRE
Plasmid#228766Purposeinsert a blue fluorescent protein mBFP2, a cyclin-dependent kinase inhibitor p27KDepositorInsertp27 kinase inhibitor (Cdkn1b Mouse)
UseTagsmTagBFP2ExpressionMammalianMutationPromoterAvailable sinceMay 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
IscB-v3
Plasmid#227015PurposeVector encoding human codon-optimized enhanced-activity OgeuIscB driven by CMV promoterDepositorInsertSV40 NLS-OgeuIscB-v3-nucleoplasmin NLS
UseTagsExpressionMutationD96R/E84R/V159RPromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-NG-ABE8.17-m+V106W
Plasmid#198534PurposeExpresses human codon-optimized SpCas9-NG-ABE8.17-m+V106W and blasticidin resistance: EFS promoter-SpCas9-NG-ABE8.17-m+V106W-NLS-FLAG-P2A-BSDDepositorInsertSpCas9-NG-ABE8.17-m+V106W
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationPromoterEFSAvailable sinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
p3E-IRES-mTagBFP2-pA (JDW 1361)
Plasmid#224535PurposeA Gateway compatible 3' entry clone containing an IRES-FLAG-NLS-mTagBFP2-pADepositorInsertIRES-FLAG-NLS-mTagBFP2-pA
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCM0-109
Plasmid#218200PurposeSV40 NLS for protein targeting to nucleusDepositorInsertSV40 NLS
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCM0-054
Plasmid#218204PurposeSV40 NLS for protein targeting to nucleusDepositorInsertSV40 NLS
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMBS-844
Plasmid#217998PurposeNucleoplasmin NLS for protein targeting to nucleusDepositorInsertNucleoplasmin NLS
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
Zed_vector_tol2_+15.2prox1a:EGFP_XCA:DsRed2
Plasmid#218206PurposeZebrafish +15.2prox1a enhancer reporter in the FCLV from 3 dpf. XCA:DsRed2 as a control in the skeletal and cardiac muscle. Shows the high background typical of the ZED vectorDepositorInsert+15.2prox1a
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
Zed_vector_tol2_-2.1prox1a_2:EGFP_XCA:DsRed2
Plasmid#218208PurposeZebrafish -2.1prox1a_2 (not mouse conserved) enhancer reporter in the facial lymphatics at 5 dpf. XCA:DsRed2 as a control in the skeletal and cardiac muscle. Shows the high background typical of the ZED vectorDepositorInsert-2.1prox1a_2
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
TDP-43 _CTD_6F→A
Plasmid#194269PurposeExpresses 6xHis-tagged C-terminal domain of TDP-43 with 6F→ADepositorInsertTDP-43 (TARDBP Human, Synthetic)
UseTags6XHistagExpressionBacterialMutationF276A, F283A, F289A, F367A, F397A, F401APromoterT7Available sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
TDP-43 _CTD_5M→A_CR
Plasmid#194263PurposeExpresses 6xHis-tagged C-terminal domain of TDP-43 with 5M→A_CRDepositorInsertTDP-43 (TARDBP Human, Synthetic)
UseTags6XHistagExpressionBacterialMutationM322A, M323A, M336A, M337A, M339A mutations in TD…PromoterT7Available sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
TDP-43 _CTD_5M→A_IDR
Plasmid#194264PurposeExpresses 6xHis-tagged C-terminal domain of TDP-43 with 5 Methionines to AlanineDepositorInsertTDP-43 (TARDBP Human, Synthetic)
UseTags6XHistagExpressionBacterialMutationM307A, M311A, M359A, M405A, M414A mutations in TD…PromoterT7Available sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
TDP-43 _CTD_5M→L_IDR
Plasmid#194265PurposeExpresses 6xHis-tagged C-terminal domain of TDP-43 with 5 Methionines to leucineDepositorInsertTDP-43 (TARDBP Human)
UseTags6XHistagExpressionBacterialMutationM307L, M311L, M359L, M405L, M414L mutations in TD…PromoterT7Available sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
TDP-43 _CTD_5M→Y_IDR
Plasmid#194266PurposeExpresses 6xHis-tagged C-terminal domain of TDP-43 with 5 Methionines to tyrosineDepositorInsertTDP-43 (TARDBP Human, Synthetic)
UseTags6XHistagExpressionBacterialMutationM307Y, M311Y, M359Y, M405Y, M414Y mutations in TD…PromoterT7Available sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
TDP-43 _CTD_5A→M_IDR
Plasmid#194267PurposeExpresses 6xHis-tagged C-terminal domain of TDP-43 with 5 Alanines to methionineDepositorInsertTDP-43 (TARDBP Human, Synthetic)
UseTags6XHistagExpressionBacterialMutationA297M, A367M, A381M, A388M, A391M mutations in TD…PromoterT7Available sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBridge-tyrosinase.σ3A
Plasmid#197513PurposeExpression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-3 σ3A fusion protein in yeast (yeast three-hybrid assays)DepositorInsertstyrosinase cytosolic tail
AP-3 σ3A
UseTagsGAL4-DNA binding domain fragment, HA tag, and nuc…ExpressionYeastMutationsilent substitution in codon 2 of tyrosinase tail…PromoterADH1 and MET25Available sinceMarch 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
Sniper2P
Plasmid#193857PurposeExpresses human codon-optimized Sniper2P and blasticidin resistance: EFS promoter-Sniper2P-NLS-FLAG-P2A-BSDDepositorInsertSniper2L
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationE1007LPromoterEFSAvailable sinceMarch 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pC1-ND-Cdt1-mCitrine
Plasmid#193771PurposeHuman Cdt1 (SV40 NLS localization and C-terminal mCitrine tag) with mutations which abroggate its S phase degradationDepositorInsertND-Cdt1-mCitrine (CDT1 Human)
UseTagsmCitrineExpressionMammalianMutationamino acids 1-19 deleted, ΔCy mutation (aa68-70 t…PromoterCMVAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHA853 - mec-4p::Fynempty
Plasmid#139210PurposeControl vector for Fyn kinase expression in mec-4 neuronsDepositorInsertFyn (FYN Human)
UseTagsExpressionWormMutationdeletion and frame shift to prevent Fyn expressionPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHA851 - osm-10p::Fynempty
Plasmid#139208PurposeControl vector for Fyn kinase expression in osm-10 neuronsDepositorInsertFyn (FYN Human)
UseTagsExpressionWormMutationdeletion and frame shift to prevent Fyn expressionPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
FUS_LC_charge_RtoK
Plasmid#139127Purposeexpress FUS LC with hnRNPA2-like charge patterning where all R are changed to KDepositorInsertFUS (FUS Human)
UseTagsExpressionBacterialMutationAdd K and D to FUS LC so it has the same charge p…PromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
FUS_LC_charge
Plasmid#139126Purposeexpress FUS LC with an hnRNPA2 LC-like charge patterningDepositorInsertFUS (FUS Human)
UseTagsExpressionBacterialMutationAdd R, K, D to make FUS LC have similar charge pa…PromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only