Skip to main content
Addgene
Showing: 1261 - 1280 of 10181 results

  5. pSP571

    Plasmid
    #139498
    Purpose
    CRISPR-Cas9 plasmid to generate double strand break in STL1 locus in S. cerevisiae. Expresses both Cas9 and STL1 sgRNA
    Depositor
    Serge Pelet
    Article
    Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:
    Insert
    pGPD Cas9 / sgRNA (STL162)
    Use
    Tags
    Expression
    Yeast
    Mutation
    WT
    Promoter
    pGPD
    Available since
    June 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    139498-plasmid-map-sequence-id-275585

    Enlarge

  12. BC-A1-011

    Plasmid
    #78699
    Purpose
    A strong promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of LVA-tagged GFP under the control of a weak RBS.
    Depositor
    Brian Chow
    Article
    Magaraci et al ACS Synth Biol. 2016 Apr 25.
    Insert
    BBa_J23100-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0033-GFP(LVA)-Terminator
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    78699-plasmid-map-sequence-id-171057

    Enlarge

  14. lentiCRISPR - C9orf114 sgRNA 1

    Plasmid
    #70655
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C9orf114 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
    Depositor
    David Sabatini
    Article
    Wang et al Science. 2015 Oct 15. pii: aac7041.
    Insert
    sgRNA against C9orf114
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 29, 2015
    Availability
    Academic Institutions and Nonprofits only

  15. BC-A1-009

    Plasmid
    #78696
    Purpose
    A strong promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of LVA-tagged GFP under the control of a strong RBS.
    Depositor
    Brian Chow
    Article
    Magaraci et al ACS Synth Biol. 2016 Apr 25.
    Insert
    BBa_J23100-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0034-GFP(LVA)-Terminator
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    78696-plasmid-map-sequence-id-171054

    Enlarge

Showing: 1261 - 1280 of 10181 results