Addgene

We narrowed to 3,948 results for: LYC;

Showing: 1261 - 1280 of 3948 results

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pJL1-TTc-4xDQNAT

Plasmid

#128393

Purpose

In vitro expression of tetanus toxin fragment C with C terminal 4xDQNAT glycosylation sites

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

Insert

Tetanus toxin fragment C domain

Use

Tags

4xDQNAT glycosylation tag and 6xHis tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pMDC84-SEIPIN1

Plasmid

#96979

Purpose

Express GFP-tagged Arabidopsis SEIPIN1 gene in plants.

Depositor

Kent Chapman

Article

Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

Insert

SEIPIN1 (AT5G16460 Mustard Weed)

Use

Tags

6xHis and GFP

Expression

Plant

Mutation

Promoter

35S

Available since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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pFastBac K-M33-Flag

Plasmid

#1993

Depositor

Robert Kingston

Article

Lavigne et al Mol Cell 2004 Feb 13;13(3):415-25.

Insert

M33 (Cbx2 Mouse)

Use

Tags

Flag

Expression

Insect

Mutation

Kozak sequence added before ATG

Promoter

Available since

March 23, 2006

Availability

Academic Institutions and Nonprofits only
psecBFP2

Plasmid

#62230

Purpose

encodes an inert blue fluorescent protein for use in oxidizing cellular environments including the periplasm and mammalian secretory pathway.

Depositor

Erik Snapp

Article

Costantini et al Biochem Biophys Res Commun. 2013 Jan 18;

Insert

secBFP2

Use

Tags

secbfp2

Expression

Mammalian

Mutation

Promoter

cmv

Available since

July 29, 2015

Availability

Academic Institutions and Nonprofits only
pGEMT-PT2A-Tdtomato-GFP-iRFP670

Plasmid

#111814

Purpose

Tricistronic construct: Co-express three fluorescent proteins by P2A and then T2A

Depositor

Li Qian

Article

Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

Inserts

Tdtomato
GFP
iRFP670

Use

Cloning intermediate

Tags

Expression

Mutation

Promoter

Available since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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PGRP-LCa

Plasmid

#21059

Depositor

Johann Deisenhofer

Article

Chang et al Proc Natl Acad Sci U S A. 2005 Jul 19. 1

Insert

PGRP-LCa (PGRP-LF Fly)

Use

Tags

His

Expression

Insect

Mutation

Promoter

Available since

Sept. 8, 2009

Availability

Academic Institutions and Nonprofits only
pRKVSV Rac T17N

Plasmid

#50537

Depositor

Kenneth Yamada

Article

Koivisto et al Exp Cell Res. 2004 Feb 1;293(1):68-80.

Insert

small GTPase, mutated (RAC1 Human)

Use

Tags

2X VSV

Expression

Mammalian

Mutation

T17N (dominant negative)

Promoter

CMV

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-eBE-S1

Plasmid

#101092

Purpose

Expresses eBE-S1 in mammalian cells

Depositor

Jia Chen

Article

Wang et al Cell Res. 2017 Aug 29. doi: 10.1038/cr.2

Insert

eBE-S1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

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p201N 1514

Plasmid

#47025

Purpose

Contains soybean miRNA miR1514 recognition sequence to produce siRNAs from 3' target sequences and induce RNA silencing

Depositor

Wayne Parrott

Article

Jacobs et al Plant Biotechnol J. 2015 Mar 27. doi: 10

Inserts

gma-miR1514 recognition sequence
nptII selectable marker

Use

RNAi

Tags

Expression

Mutation

Promoter

GmUbi and StUbi-3

Available since

Aug. 30, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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2842 pBS Bcl-2 5'genomic

Plasmid

#8747

Depositor

Stanley Korsmeyer

Article

Veis et al Cell 1993 Oct 22;75(2):229-40.

Insert

Bcl-2 5' genomic (Bcl2 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 6, 2005

Availability

Academic Institutions and Nonprofits only
pCARGO-RMCE

Plasmid

#233264

Purpose

Backbone plasmid used to clone lncRNA inserts in preparation for RMCE

Depositor

Mauro Calabrese

Article

Trotman et al Mol Cell. 2025 Mar 20;85(6):1117-1133.e1

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 Flag-TUBG2

Plasmid

#229855

Purpose

TUBG2

Depositor

Maria Alvarado-Kristensson

Article

Lindstrom et al FASEB J. 2025 Mar 15;39(5):e70431. doi:

Insert

TUBG2 (TUBG2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Removal of the internal TUBG2-EcoRI site by chang…

Promoter

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+ dgat1a-FLAG

Plasmid

#232143

Purpose

Expresses zebrafish dgat1a with a C-terminal FLAG tag under control of the CMV promoter

Depositor

Steven Farber

Article

Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

Insert

dgat1a (dgat1a Zebrafish)

Use

Zebrafish expression

Tags

FLAG

Expression

Mutation

Promoter

CMV

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCRII-TOPO-dgat1b in situ probe

Plasmid

#232138

Purpose

zebrafish dgat1b anti-sense in situ probe, linearize with NotI, SP6 polymerase

Depositor

Steven Farber

Article

Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

Insert

dgat1b

Use

In vitro transcription

Tags

Expression

Mutation

Promoter

SP6/T7

Available since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pCRII-TOPO-mogat2 in situ probe

Plasmid

#232139

Purpose

zebrafish mogat2 anti-sense in situ probe, linearize with NotI, SP6 polymerase

Depositor

Steven Farber

Article

Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

Insert

mogat2 (mogat2 Zebrafish)

Use

In vitro transcription

Tags

Expression

Mutation

Promoter

SP6/T7

Available since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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esgRNA_SlMIR164b

Plasmid

#231153

Purpose

T-DNA encoding TRV2 with mobile gRNA targeting SlMIR164b

Depositor

Daniel Voytas

Article

Liu et al Plant Physiol. 2024 Mar 29;194(4):2229-2

Insert

mobile gRNA targeting SlMIR164b

Use

Tags

Expression

Plant

Mutation

Promoter

PEBV sub-genomic

Available since

Jan. 15, 2025

Availability

Academic Institutions and Nonprofits only

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Talin-1 WT

Plasmid

#201798

Purpose

Expresses Talin-1 protein in MAC-Tag-C backbone

Depositor

Vesa Hytönen

Article

Turkki et al Sci Rep. 2024 Aug 27;14(1):19862. doi: 1

Insert

Talin-1 (Tln1 Mouse)

Use

Tags

BirA, HA, strepIII

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-CsHPAD-AE

Plasmid

#226982

Purpose

Expressing Clostridium scatologenes hydroxyphenylacetate decarboxylase activating enzyme (HPAD-AE) in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

CsHPAD-AE

Use

Tags

His6 (on backbone)

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-OuIAD-AE

Plasmid

#226968

Purpose

Expressing Olsenella uli indoleacetate decarboxylase activating enzyme (IAD-AE) in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

OuIAD-AE

Use

Tags

His6 (on backbone)

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-OuIAD

Plasmid

#226967

Purpose

Expressing Olsenella uli indoleacetate decarboxylase (IAD) in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

OuIAD

Use

Tags

His6 (on backbone)

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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