We narrowed to 1,469 results for: MiCy

1. pFastBac1_GI.1_Q141V-P221L

   Plasmid

   #162584

   Purpose

   Expresses norovirus GI.1 VP1 protein with mutations Q141V-P221L in Sf9 insect cells

   Depositor

   Peter Kwong

   Article

   Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

   Insert

   VP1 protein from human norovirus GI.1
   

   Expression

   Insect

   Mutation

   Glutamine 141 changed to Valine and Proline 221 c…

   Promoter

   polyhedrin

   Available Since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFastBac1_GI.1_L144C-P221C

   Plasmid

   #162585

   Purpose

   Expresses norovirus GI.1 VP1 protein with mutations L144C-P221C in Sf9 insect cells

   Depositor

   Peter Kwong

   Article

   Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

   Insert

   VP1 protein from human norovirus GI.1
   

   Expression

   Insect

   Mutation

   Leucine 144 changed to Cysteine and Proline 221 c…

   Promoter

   polyhderin

   Available Since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFastBac1_GI.1_G131C-N172C

   Plasmid

   #162586

   Purpose

   Expresses norovirus GI.1 VP1 protein with mutations G131C-N172C in Sf9 insect cells

   Depositor

   Peter Kwong

   Article

   Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

   Insert

   VP1 protein from human norovirus GI.1
   

   Expression

   Insect

   Mutation

   Glycine 131 changed to Cysteine and Asparagine 17…

   Promoter

   polyhedrin

   Available Since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFastBac1_GI.1_N167C-L169C

   Plasmid

   #162587

   Purpose

   Expresses norovirus GI.1 VP1 protein with mutations N167C-L169C in Sf9 insect cells

   Depositor

   Peter Kwong

   Article

   Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

   Insert

   VP1 protein from human norovirus GI.1
   

   Expression

   Insect

   Mutation

   Asparagine 167 changed to Cysteine and Leucine 16…

   Promoter

   polyhedrin

   Available Since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEN_TT 3xFLAG-MAVS-Gln93

   Plasmid

   #158629

   Purpose

   Gateway entry vector for an inducible 3xFLAG-tagged MAVS mutant

   Depositor

   Kevin Janes

   Article

   Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

   Insert

   MAVS (MAVS Human)
   

   Use

   Gateway entry vector

   Tags

   3XFLAG

   Mutation

   E93Q

   Available Since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEN_TT 3xFLAG-MAVS-Ala148

   Plasmid

   #158630

   Purpose

   Gateway entry vector for an inducible 3xFLAG-tagged MAVS mutant

   Depositor

   Kevin Janes

   Article

   Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

   Insert

   MAVS (MAVS Human)
   

   Use

   Gateway entry vector

   Tags

   3XFLAG

   Mutation

   Q148A

   Available Since

   Sept. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFastBac1-OSF-TRIM5alpha(African green monkey pygerythrus)(1-293)

   Plasmid

   #79030

   Purpose

   Baculoviral entry vector to produce Bac-to-bac baculoviruses

   Depositor

   Wesley Sundquist

   Article

   Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

   Insert

   OSF-TRIM5alpha(African green monkey pygerythrus)(1-293)
   

   Tags

   OneSTrEP-FLAG

   Expression

   Insect

   Mutation

   deleted amino acid 294-515 from frican green monk…

   Promoter

   polyhedrin

   Available Since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFastBac-ATG101-ATG13 HORMA

   Plasmid

   #99332

   Purpose

   Mammalian expression of one subcomplex from the Human ULK1 complex

   Depositor

   James Hurley

   Article

   Qi et al Structure. 2015 Oct 6;23(10):1848-57. do

   Inserts

   ATG101 (ATG101 Human)
   ATG13 (ATG13 Human)
   

   Tags

   GST

   Expression

   Insect

   Promoter

   p10 and pH

   Available Since

   Aug. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pinducer 20 DN-KASH

   Plasmid

   #125554

   Purpose

   Tet-ON inducible lentivirus expressing mcherry-labeled DN KASH

   Depositor

   Daniel Conway

   Article

   Mayer et al Cell Mol Bioeng. 2019 Jul 11;12(4):289-3

   Insert

   SYNE1 (SYNE1 Human)
   

   Use

   Lentiviral

   Tags

   mcherry

   Mutation

   Truncated form: only the KASH domain of nesprin-…

   Promoter

   tetracycline responsive element

   Available Since

   July 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDyn1

    Plasmid

    #64067

    Purpose

    Human cytoplasmic dynein heavy chain (DYNC1H1) codon optimized for Sf9 expression with a SNAPf tag for fluorescent labeling and a TEV-cleavable His-ZZ tag for purification (His-ZZ-TCS-SNAPf-DYNC1H1).

    Depositor

    Andrew Carter

    Article

    Schlager et al EMBO J. 2014 Sep 1;33(17):1855-68. doi:

    Insert

    DYNC1H1 (DYNC1H1 Human)
    

    Use

    Cre/Lox

    Tags

    His-ZZ-TCS-SNAPf

    Expression

    Insect

    Mutation

    Codon optimized for Sf9

    Available Since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAcBac1-Mb-sfGFP WT

    Plasmid

    #197569

    Purpose

    Expression of sfGFP WT with Methanosarcina barkeri (Mb) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cells

    Depositor

    Ryan Mehl

    Article

    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

    Inserts

    sfGFP WT
    Pyl-tRNA (4 copies)
    

    Tags

    V5-His6

    Expression

    Mammalian

    Promoter

    CMV and U6/H1

    Available Since

    April 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFastbac1-GST-hUSP7

    Plasmid

    #63573

    Purpose

    Expression of synthetic human USP7 tagged with GST

    Depositor

    Titia Sixma

    Article

    Faesen et al Mol Cell. 2011 Oct 7;44(1):147-59. doi:

    Insert

    USP7 (USP7 Human, Synthetic)
    

    Tags

    GST

    Expression

    Insect

    Mutation

    Synthetic based on human protein sequence (please…

    Promoter

    Polyhedrin

    Available Since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAcBac1-Mb-Fluoro-Phe D6

    Plasmid

    #197577

    Purpose

    Expression of Methanosarcina barkeri (Mb) Fluoro-Phe D6 tRNA synthetase/Pyl-tRNA pair for the encoding of fluorinated phenylalanine derivatives at TAG codons in HEK293 cells

    Depositor

    Ryan Mehl

    Article

    Galles et al Nat Commun. 2023 Jan 4;14(1):59. doi: 10

    Inserts

    Mb Pyl Fluoro-Phe B5 synthetase
    Pyl-tRNA (4 copies)
    

    Tags

    Nuclear export sequence + FLAG

    Expression

    Mammalian

    Mutation

    N311A, C313M, V366G, W382T

    Promoter

    CMV and U6/H1

    Available Since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEN_TT 3XFLAG-wtGATA6-3XAU1

    Plasmid

    #72612

    Purpose

    Gateway entry vector for an inducible N-terminally 3XFLAG-tagged and C-terminally AU1-tagged human wtGATA6

    Depositor

    Kevin Janes

    Article

    Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

    Insert

    GATA binding protein 6 (GATA6 Human)
    

    Use

    Entry vector for gateway cloning

    Tags

    3XAU1 and 3XFLAG

    Expression

    Bacterial

    Promoter

    TRE tight promoter

    Available Since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAcBac1-Ma-sfGFP 150TAG

    Plasmid

    #197568

    Purpose

    Expression of sfGFP-150TAG with Methanomethylophilus alvus (Ma) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cells

    Depositor

    Ryan Mehl

    Article

    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

    Inserts

    sfGFP 150TAG
    M. alvus Pyl-tRNA (6) (4x copies)
    

    Tags

    V5-His6

    Expression

    Mammalian

    Mutation

    N150TAG

    Promoter

    CMV and U6/H1

    Available Since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pACEBac1-PSMA1-PSMA2-PSMA3-PSMA4

    Plasmid

    #217893

    Purpose

    Insect cell expression of human 20S CP proteasome subunit alpha type-1 (PSMA1), alpha type-2 (PSMA2), alpha type-3 (PSMA3), and alpha type-4 (PSMA4)

    Depositor

    Brenda Schulman

    Article

    Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1

    Inserts

    PSMA1 (PSMA1 Human)
    PSMA2 (PSMA2 Human)
    PSMA3 (PSMA3 Human)
    PSMA4 (PSMA4 Human)
    

    Expression

    Insect

    Available Since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFB1.HMBP.PrS.dEZH2

    Plasmid

    #220241

    Purpose

    Expression of an EZH2 catalytic site mutant in insect cells.

    Depositor

    Chen Davidovich

    Article

    Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

    Insert

    dEZH2 (EZH2 Human)
    

    Tags

    6x His, MBP

    Expression

    Insect

    Mutation

    Y731F

    Promoter

    Polyhedrin

    Available Since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pACEBac1-gamma-TuSC

    Plasmid

    #178079

    Purpose

    Insect cell expression of gamma-TuSC

    Depositor

    Tarun Kapoor

    Article

    Wieczorek et al J Cell Biol. 2021 Mar 1;220(3). pii: 211

    Inserts

    gamma-tubulin (TUBG1 Human)
    GCP2 (TUBGCP2 Human)
    GCP3 (TUBGCP3 Human)
    

    Tags

    TEV-His6

    Expression

    Insect

    Available Since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMMACE DEST CMV H2B iRFP

    Plasmid

    #206253

    Purpose

    DEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes H2B iRFP713 under the control of CMV promoter. CRE-Acceptor in MultiBac system.

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    H2B iRFP713
    

    Use

    Recombinant baculovirus production, multimate/gat…

    Tags

    iRFP713

    Expression

    Mammalian

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. ReactionC_pBIG1a_ATG7_ATG10

    Plasmid

    #190860

    Purpose

    Plasmid for the expression and purification of ATG7 and ATG10 protein subunit complexes. Internal reference: SMC1179

    Depositor

    Sascha Martens

    Article

    Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

    Inserts

    ATG7 (ATG7 Human)
    ATG10 (ATG10 )
    

    Expression

    Bacterial and Insect

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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