We narrowed to 5,531 results for: cdna expression plasmid

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pcDNA-FRT-TO-FH-Nsp8-Nsp7_GC3opt

Plasmid

#157701

Purpose

mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp8-Nsp7 fusion protein under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

FH-Nsp8-Nsp7_GC3opt (ORF1ab SARS-CoV-2)

Tags

Flag-His8

Expression

Mammalian

Mutation

codon optimized; gene expression was optimized by…

Available Since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA 3.1(-) mouse C/EBP beta (LIP)

Plasmid

#12561

Depositor

Peter Johnson

Insert

CCAAT/Enhancer-binding Protein beta LIP (Cebpb Mouse)

Expression

Mammalian

Available Since

July 18, 2007

Availability

Academic Institutions and Nonprofits only
RaCCR1_EYFP_pcDNA3.1

Plasmid

#159122

Purpose

This plasmid encodes RaCCR1 rhodopsin domain

Depositor

John Spudich

Article

Sineshchekov et al mBio. 2020 Apr 21;11(2). pii: mBio.00657

Insert

RaCCR1

Tags

EYFP

Expression

Mammalian

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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RsCCR2_EYFP_pcDNA3.1

Plasmid

#159125

Purpose

This plasmid encodes RsCCR2 rhodopsin domain

Depositor

John Spudich

Article

Sineshchekov et al mBio. 2020 Apr 21;11(2). pii: mBio.00657

Insert

RsCCR2

Tags

EYFP

Expression

Mammalian

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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RsCCR1_EYFP_pcDNA3.1

Plasmid

#159124

Purpose

This plasmid encodes RsCCR1 rhodopsin domain

Depositor

John Spudich

Article

Sineshchekov et al mBio. 2020 Apr 21;11(2). pii: mBio.00657

Insert

RsCCR1

Tags

EYFP

Expression

Mammalian

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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RaCCR2_EYFP_pcDNA3.1

Plasmid

#159123

Purpose

This plasmid encodes RaCCR2 rhodopsin domain

Depositor

John Spudich

Article

Sineshchekov et al mBio. 2020 Apr 21;11(2). pii: mBio.00657

Insert

RaCCR2

Tags

EYFP

Expression

Mammalian

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_SARS2_BQ.1.1

Plasmid

#194493

Purpose

expressing SARS-CoV-2 BQ.1.1 spike protein for pseudovirus production

Depositor

David Nemazee

Article

Zhou et al Immunity. 2023 Mar 14;56(3):669-686.e7.

Insert

Spike (BQ.1.1) (S SARS-CoV-2)

Expression

Mammalian

Mutation

R346T, K444T, N460K and c-terminal 18aa del on th…

Promoter

CMV

Available Since

Jan. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-SARS-CoV-2-S-ectodomain

Plasmid

#235984

Purpose

Internally tagged SARS-CoV-2 spike ecto-domain

Depositor

Syed Saif Hasan

Article

Singh et al J Struct Biol X. 2025 Feb 11;11:100123.

Insert

Spike protein (S SARS-CoV-2)

Tags

8xHis and Twin-Strep tag and internal Twin-Strep …

Expression

Mammalian

Mutation

aa 1-1208, D614G, RRAR684-684GSAS, and DK987-987P…

Promoter

CMV

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_CoV2_D18

Plasmid

#170442

Purpose

Express SARS-CoV-2 WT spike protein with an 18aa deletion on the C-terminal tail. Used for SARS2 pseudotyped virus production.

Depositor

David Nemazee

Article

Rogers et al Science. 2020 Aug 21;369(6506):956-963.

Insert

SARS-CoV-2 spike D18 (S SARS-CoV-2, USA-WA1/2020 strain)

Tags

NA

Expression

Mammalian

Mutation

18aa deletion in c-terminal tail

Promoter

CMV

Available Since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-N-Myc_1-1083 USP7

Plasmid

#131251

Purpose

Mammalian expression of N-terminally Myc-tagged USP7, minus the C-terminal 18 amino acids

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)

Tags

Myc

Expression

Mammalian

Mutation

A mutant form of our pcDNA3.1-N-Myc_WT USP7 plasm…

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_3xFlagEzh2 WT

Plasmid

#173717

Purpose

WT EZH2 expression plasmid with 3XFLAG tag

Depositor

Thomas Cech

Article

Long et al Nat Genet. 2020 Sep;52(9):931-938. doi:

Insert

EZH2 (EZH2 Human)

Tags

3XFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 SARS-CoV-2 N

Plasmid

#158079

Purpose

Expresses SARS-CoV-2 nucleocapsid protein in mammalian cells

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Insert

SARS-CoV-2 nucleocapsid (N )

Expression

Mammalian

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 SARS-CoV-2 E

Plasmid

#158080

Purpose

Expresses SARS-CoV-2 envelope protein in mammalian cells

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Insert

SARS-CoV-2 envelope (E )

Expression

Mammalian

Available Since

June 15, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 SARS-CoV-2 M

Plasmid

#158078

Purpose

Expresses SARS-CoV-2 membrane protein in mammalian cells

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Insert

SARS-CoV-2 matrix (M )

Expression

Mammalian

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1-NbON10-NanoLuc

Plasmid

#198691

Purpose

Expresses anti-SARS-Cov-2 Nucleocapsid nanobody ON10 fused to NanoLuc in HEK293 cell culture

Depositor

Gualberto González Sapienza

Article

Segovia-de Los Santos et al Front Immunol. 2023 Jul 11;14:1220477. d

Insert

anti-SARS-CoV-2 Nucleocapsid nanobody ON10

Tags

6xHis tag, IgK leader sequence, NanoLuc, and twin…

Expression

Mammalian

Promoter

CMV promoter

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-α-COPI-WD40-Arg57Ala

Plasmid

#180759

Purpose

Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Arg57Ala (residues 1-327)

Depositor

Syed Saif Hasan

Article

Dey et al Commun Biol. 2022 Feb 8;5(1):115. doi: 1

Insert

α-COPI-WD40-Arg57Ala

Tags

C-terminal strep tag

Expression

Mammalian

Mutation

Encodes residues 1-327; mutations: Arg57Ala, Leu1…

Promoter

CMV

Available Since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-α-COPI-WD40-Tyr139Ala

Plasmid

#180761

Purpose

Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Tyr139Ala (residues 1-327)

Depositor

Syed Saif Hasan

Article

Dey et al Commun Biol. 2022 Feb 8;5(1):115. doi: 1

Insert

α-COPI-WD40-Tyr139Ala

Tags

C-terminal strep tag

Expression

Mammalian

Mutation

Encodes residues 1-327; mutations: Tyr139Ala, Leu…

Promoter

CMV

Available Since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-α-COPI-WD40-Asp115Ala

Plasmid

#180760

Purpose

Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Asp115Ala (residues 1-327)

Depositor

Syed Saif Hasan

Article

Dey et al Commun Biol. 2022 Feb 8;5(1):115. doi: 1

Insert

α-COPI-WD40-Asp115Ala

Tags

C-terminal strep tag

Expression

Mammalian

Mutation

Encodes residues 1-327; mutations: Asp115Ala, Leu…

Promoter

CMV

Available Since

March 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA4TO-3XFLAG Halo R99A nsp1

Plasmid

#175422

Purpose

Expresses R99A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

CoV2 R99A nsp1

Tags

3XFLAG Halo

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1(-) rat C/EBP beta (LAP)

Plasmid

#12558

Depositor

Peter Johnson

Insert

CCAAT/Enhancer-binding Protein beta (Cebpb Rat)

Expression

Mammalian

Available Since

July 18, 2007

Availability

Academic Institutions and Nonprofits only