We narrowed to 4,785 results for: pcr
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Plasmid#213513PurposeattB-containing vector backbone, for use in restriction enzyme- or PCR-based cloningDepositorTypeEmpty backboneUseZebrafish expressionAvailable SinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only
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pFA CUP1p-NuiHA kanMX
Plasmid#131169PurposePCR template for genomic ScCUP1 promoter regulated N-terminal Nui-HA prey fusion, kanMX marker confers resistance to geneticin in S. cerevisiaeDepositorTypeEmpty backboneTagsNui-HAExpressionYeastAvailable SinceDec. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRL093 pattB_exorh:EGFP_MCS
Plasmid#213514PurposeattB-containing vector backbone, for use in restriction enzyme- or PCR-based cloningDepositorTypeEmpty backboneUseZebrafish expressionAvailable SinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC19-TgKI-MCS-tdTomato-polyA
Plasmid#174022PurposeThis plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.DepositorInserttdTomato
UseZebrafish knock-in taggingAvailable SinceNov. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
LBJJ383
Plasmid#117517PurposePCR template for sgRNA classic with polyT as terminatorDepositorInsertBpiI:ACTA:pU6-26:sgRNA:polyT:TTAC:BpiI
UseCRISPRExpressionPlantPromoterAtU6-26Available SinceOct. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
Monomer NH_V2
Plasmid#37527Purposetemplate for PCR amplification of the monomer used for assembly of the DNA-binding tandem repeat region of the TALE.DepositorInsertMonomer NH
Available SinceAug. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMOTag-glmS-4H
Plasmid#106378PurposeCan be used as a PCR template to amplify the glmS-HA fragment for gene specific tagging and regulationDepositorInsertGlmS-3xHA
Available SinceMarch 2, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFA6a-FKBP-GFP-HIS3MX6
Plasmid#183023PurposePCR-based tagging of yeastDepositorInsertFKBP
UseYeast genomic targetingAvailable SinceMay 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pECT3
Plasmid#75450PurposePlasmid that serves as the PCR template to create genomic pdt3 insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicateDepositorInsertpdt3
ExpressionBacterialPromoternoneAvailable SinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEP-EGFPin
Plasmid#60961PurposePCR template for the insertion of eGFP by en passant (two-step Red-mediated) mutagenesisDepositorInserteGFP-Kana_I-SceI
Available SinceMarch 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUC19-TgKI-MCS-mLanYFP-polyA
Plasmid#174021PurposeThis plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.DepositorInsertmLanYFP
UseZebrafish knock-in taggingAvailable SinceNov. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCfB8742
Plasmid#126908PurposeTemplate for PCR amplification of pGPD1-pFBA1 double promoter biobrick for Yarrowia lipolyticaDepositorInsertpGPD1 and pFBA1
UseSynthetic BiologyAvailable SinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pECT5
Plasmid#75451PurposePlasmid that serves as the PCR template to create genomic pdt5 insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicateDepositorInsertpdt5
ExpressionBacterialPromoternoneAvailable SinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
U6>XX
Plasmid#87542PurposeComponent 1 of 2 for U6>sgRNA cassette OSO-PCRDepositorTypeEmpty backboneAvailable SinceJan. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pECM1
Plasmid#75445PurposePlasmid that serves as the PCR template to create the genomic pdt1 insertions contained in the EPD library. Plasmid contains the R6K origin of replication so needs pir gene to replicateDepositorInsertpdt1
ExpressionBacterialPromoternoneAvailable SinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJUNB-donor
Plasmid#112313PurposeCRISPR donor plasmid to tag human transcription factor JUNB with GFPDepositorInsertJUNB homology arms flanking EGFP-IRES-Neo cassette (JUNB Human)
UseCRISPRMutationhg38:19:12792992:C>G, hg38:19:12793018:T>G,…Available SinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNL2080 pGATA23 GG0-GG1
Plasmid#195908PurposeLevel 0 pGATA23 promoter with GG0 and GG1 golden gate linkersDepositorInsertpGATA23
UseSynthetic BiologyAvailable SinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAct1IHPT-4
Plasmid#37844DepositorInsertActin promoter and intron
UsePlant transformationTagsHPT (Hygromycin phosphotransferase) and nos 3…Available SinceNov. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pECT1
Plasmid#75446PurposePlasmid that serves as the PCR template to create genomic pdt1 insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicateDepositorInsertpdt1
ExpressionBacterialPromoternoneAvailable SinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBALU-ext
Plasmid#69339PurposeIt allows to extend the genomic insert in a fosmid.DepositorInsertkanamycin gene +pCC1FOS homology
ExpressionBacterialAvailable SinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only