We narrowed to 6,901 results for: plasmid dna

1. RGS-6xHis-LIN-29-pcDNA3.1-

   Plasmid

   #52515

   Purpose

   expresses C. elegans LIN-29 in mammalian cells with RGS-6xHis tag at N-terminus

   Depositor

   Adam Antebi

   Article

   Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

   Insert

   Lin-29 b (lin-29 Nematode)
   

   Use

   Tags

   RGS-6xHis

   Expression

   Mammalian

   Mutation

   wt (changed coding sequence in 5' (5th codon…

   Promoter

   CMV

   Available Since

   Aug. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. (302) pcDNA3.1(+)-HA-LaG6-(EAAAK)4-OGT(4.5)

   Plasmid

   #168192

   Purpose

   HA-tagged GFP nanobody Lag6 (310 nM affinity) fused to a truncated OGT of 4 TPRs

   Depositor

   Christina Woo

   Article

   Ramirez et al Curr Protoc. 2021 May;1(5):e117. doi: 10

   Insert

   HA-LaG6-(EAAAK)4-OGT(4)
   

   Use

   Tags

   HA-Tag

   Expression

   Mammalian

   Mutation

   Promoter

   T7/CMV

   Available Since

   May 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-Tras heavy chain-GSG-SpyTag003

   Plasmid

   #216292

   Purpose

   Expresses anti-HER2 Tras Heavy chain-GSG-SpyTag003 in mammalian cells. To be paired with Tras light chain to form the anti-HER2 Tras Fab

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   Tras heavy chain-GSG-SpyTag003
   

   Use

   Affinity Reagent/ Antibody

   Tags

   His6 tag, Signal peptide, and SpyTag003

   Expression

   Mammalian

   Mutation

   Promoter

   T7 promoter

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1(+)-C-6His-mEsrra

   Plasmid

   #173152

   Purpose

   mouse estrogen-related receptor alpha (Esrra) expression plasmid in mammalian cells

   Depositor

   Brijesh Kumar Singh

   Article

   Tripathi et al bioRxiv 2021.11.16.468891

   Insert

   ORF clone Estrogen-related receptor alpha (Esrra) (Esrra Mouse)
   

   Use

   Tags

   His

   Expression

   Mammalian

   Mutation

   Wild type

   Promoter

   Available Since

   Aug. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA3 ATP5O(5’UTR)-FF

   Plasmid

   #85486

   Purpose

   Firefly luciferase under the control of ATP5O 5'UTR

   Depositor

   Ivan Topisirovic

   Article

   Gandin et al Genome Res. 2016 May;26(5):636-48. doi:

   Insert

   5'UTR of ATP5O (ATP5PO Human)
   

   Use

   Luciferase

   Tags

   Firefly luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA3 ATP5O(5’UTR)-SL-FF

   Plasmid

   #85491

   Purpose

   Firefly luciferase under the control of ATP5O 5'UTR followed by a stem-loop

   Depositor

   Ivan Topisirovic

   Article

   Gandin et al Genome Res. 2016 May;26(5):636-48. doi:

   Insert

   5'UTR of ATP5O followed by stem loop (ATP5PO Human)
   

   Use

   Luciferase

   Tags

   Firefly luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA3 ATP5O(TISU)-FF

   Plasmid

   #85489

   Purpose

   Firefly luciferase under the control of ATP5O TISU

   Depositor

   Ivan Topisirovic

   Article

   Gandin et al Genome Res. 2016 May;26(5):636-48. doi:

   Insert

   Full TISU element of ATP5O (ATP5PO Human)
   

   Use

   Luciferase

   Tags

   Firefly luciferase

   Expression

   Mammalian

   Mutation

   2nd and 3rd codon of luciferase were modified fro…

   Promoter

   Available Since

   Jan. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA3 UQCC2(5’UTR)-FF

   Plasmid

   #85487

   Purpose

   Firefly luciferase under the control of UQCC2 5'UTR

   Depositor

   Ivan Topisirovic

   Article

   Gandin et al Genome Res. 2016 May;26(5):636-48. doi:

   Insert

   5'UTR of UQCC2 (C6orf125 Human)
   

   Use

   Luciferase

   Tags

   Firefly luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. (189) pcDNA3.1-HA-nGFP-(EAAAK)4-OGT(4)

   Plasmid

   #160739

   Purpose

   Expresses a truncated OGT (4.5 TPRs) fused to a GFP nanobody

   Depositor

   Christina Woo

   Article

   Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-10

   Insert

   HA-nGFP-(EAAAK)4-OGT(4)
   

   Use

   Tags

   HA-Tag

   Expression

   Mammalian

   Mutation

   Promoter

   T7/CMV

   Available Since

   May 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. (137) pcDNA3.1-HA-nEPEA-(EAAAK)4-OGT(4)

    Plasmid

    #160740

    Purpose

    Expresses a truncated OGT (4.5 TPRs) fused to the EPEA nanobody

    Depositor

    Christina Woo

    Article

    Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-10

    Insert

    HA-nEPEA-(EAAAK)4-OGT(4)
    

    Use

    Tags

    HA-Tag

    Expression

    Mammalian

    Mutation

    Promoter

    T7/CMV

    Available Since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. (81) pcDNA3.1-BC2-Nup62-Flag-EPEA

    Plasmid

    #168094

    Purpose

    Nucleoporin Nup62 with N-terminal BC2 nanobody tag and C-terminal Flag and EPEA nanobody tags

    Depositor

    Christina Woo

    Article

    Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

    Insert

    Nup62-Ubc-Flag-EPEA
    

    Use

    Tags

    BC2-Tag, EPEA-tag, and Flag-tag

    Expression

    Mammalian

    Mutation

    P229A, S283T on Nup62 native sequence

    Promoter

    T7/CMV

    Available Since

    May 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.2 mir-1-1 reporter hsa-mir17~20

    Plasmid

    #46671

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    hsa-mir17~20 (MIR17 Human)
    

    Use

    RNAi

    Tags

    V5 and hsa-mir-1-1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. (112) pcDNA3.1-GFP-Flag-Nup62-EPEA

    Plasmid

    #168101

    Purpose

    GFP and Flag tagged Nup62 with EPEA nanobody tag

    Depositor

    Christina Woo

    Article

    Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

    Insert

    GFP-Flag-Nup62-EPEA
    

    Use

    Tags

    EPEA, Flag, and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    T7/CMV

    Available Since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-CD40L (lose of function mutant)-mgSrtA

    Plasmid

    #125796

    Purpose

    for co-expression of a loss of function mutant of CD40L and mgSrtA on cell membrane. A negative control to plasmid pcDNA3.1-CD40L-mgSrtA.

    Depositor

    P. Chen

    Article

    Ge et al J Am Chem Soc. 2019 Feb 6;141(5):1833-18

    Insert

    CD40L (lose of function mutant)-mgSrtA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-CAG-LMO1 (GLuc-ChR2-EYFP)

    Plasmid

    #72888

    Purpose

    fusion protein of Gaussia luciferase, Channelrhodopsin-2, and enhanced yellow fluorescent protein (luminopsin) for bioluminescent optogenetics

    Depositor

    Ute Hochgeschwender

    Article

    Berglund et al PLoS One. 2013;8(3):e59759. doi: 10.1371

    Insert

    Gaussia luciferase, Channelrhodopsin-2, EYFP
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3_SARS-CoV-Spike[SARS-CoV-2 RBD]

    Plasmid

    #190086

    Purpose

    express chimeric SARS-CoV spike with the SARS-CoV-2 receptor binding domain

    Depositor

    Kenneth Matreyek

    Article

    Roelle et al PLoS Biol. 2022 Jul 27;20(7):e3001738. d

    Insert

    SARS-CoV S (S Synthetic)
    

    Use

    Tags

    Rhodopsin C9

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. (366) pcDNA3.1(+)-HA-nUbc-(EAAAK)4-OGT(4, K852A)

    Plasmid

    #168189

    Purpose

    HA-tagged Ubc6E nanobody fused to an inactive truncated OGT of 4 TPRs with K852A mutation

    Depositor

    Christina Woo

    Article

    Ramirez et al Curr Protoc. 2021 May;1(5):e117. doi: 10

    Insert

    HA-nUbc6E-(EAAAK)4-OGT(4,K852A)
    

    Use

    Tags

    HA-Tag

    Expression

    Mammalian

    Mutation

    Promoter

    T7/CMV

    Available Since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.2 mir-1-1 reporter dme-mir-125

    Plasmid

    #46667

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    dme-mir-125
    

    Use

    RNAi

    Tags

    V5 and hsa-mir-1-1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1-CMV-LMO2 (GLuc-VChR1-EYFP)

    Plasmid

    #72889

    Purpose

    fusion protein of Gaussia luciferase, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein (luminopsin LMO2) for bioluminescent optogenetics

    Depositor

    Ute Hochgeschwender

    Article

    Berglund et al PLoS One. 2013;8(3):e59759. doi: 10.1371

    Inserts

    Gaussia luciferase, Volvox channelrhodopsin 1, EYFP
    Volvox channelrhodopsin 1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.2 mir-1-1 reporter dme-mir-279

    Plasmid

    #46668

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    dme-mir-279
    

    Use

    RNAi

    Tags

    V5 and hsa-mir-1-1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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