We narrowed to 1,231 results for: POLI

1. FUW-C-prM-E-NS1

   Plasmid

   #175281

   Purpose

   lentiviral vector mediating bicistronic expression of the 4 genes of YFV-17D (C-prM-E-NS1) and EGFP.

   Depositor

   Wei Xu

   Article

   Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

   Insert

   C-prM-E-NS1 and EGFP (POLY Yellow fever virus strain 17D)
   

   Use

   Lentiviral

   Tags

   IRES EGFP

   Promoter

   human ubiquitin C

   Available Since

   Dec. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV-Syn-NS1

   Plasmid

   #175276

   Purpose

   AAV vector mediating bicistronic expression of NS1 gene of YFV-17D and dTomato with NLS

   Depositor

   Wei Xu

   Article

   Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

   Insert

   Nonstructural protein 1 (NS1) of YFV-17D; NLS-dTomato (POLY Yellow fever virus strain 17D)
   

   Use

   AAV

   Tags

   NLS-dTomato (P2A cleavage)

   Promoter

   Synapsin

   Available Since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28_6xHis-PARP1delZn2delBRCT

   Plasmid

   #173939

   Purpose

   Bacterial expression of PARP1 lacking Zn2 and BRCT domains

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1delZn2delBRCT (PARP1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Deletion of residues 97-213 and 374-520; V762A

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28_6xHis-PARP1-E988K

   Plasmid

   #173945

   Purpose

   Bacterial expression of inactive PARP1 mutant (E988K attenuates catalytic and PAR-branching activity of PARP1)

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-E988K (PARP1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   V762A, E988K

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28_6xHis-PARP1-CATdeltaHD

   Plasmid

   #174811

   Purpose

   Bacterial expression of a hyperactive PARP1 CAT lacking the autoinhibitory HD subdomain

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-CATdeltaHD (PARP1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Residues 678-787 replaced by eight-residue linker…

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-TRE-NS1-dTomato

   Plasmid

   #175278

   Purpose

   AAV vector mediating inducible bicistronic expression of NS1 gene of YFV-17D and dTomato

   Depositor

   Wei Xu

   Article

   Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

   Insert

   NS1; dTomato (POLY Yellow fever virus strain 17D)
   

   Use

   AAV

   Tags

   dTomato (from bidirectional promoter)

   Promoter

   bidirectional TRE promoter

   Available Since

   Dec. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-DIO-NS1

   Plasmid

   #175273

   Purpose

   AAV vector mediating Cre-depenent expression of NS1 gene of YFV-17D

   Depositor

   Wei Xu

   Article

   Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

   Insert

   Nonstructural protein 1 (NS1) of YFV-17D (POLY Yellow fever virus strain 17D)
   

   Use

   AAV

   Promoter

   Synapsin

   Available Since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-SCP1-dSa VPR mini.-2X snRP-1 Neurog2

   Plasmid

   #99694

   Purpose

   Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Actc1, vector allows for strong activation of mouse Actc1, can be packaged and delivered as AAV

   Depositor

   George Church

   Article

   Vora et al bioRxiv 2018. 298620

   Insert

   dCas9 and gRNA targeting Actc1 (gRNA: GGTATATAAGGGGTTTTAAG) (Actc1 Synthetic, Mouse)
   

   Use

   AAV and CRISPR

   Tags

   VPR mini

   Mutation

   dead Cas9

   Available Since

   Jan. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28_6xHis-PARP1-CAT-L713F

   Plasmid

   #173944

   Purpose

   Bacterial expression of isolated PARP1 CAT domain containing L713F gain-of-function mutation

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-CAT-L713F (PARP1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   L713F, V762A

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28_6xHis-PARP1-L713F

    Plasmid

    #174794

    Purpose

    Bacterial expression of a hyperactive PARP1 mutant (L713F destabilizes the autoinhibitory HD subdomain)

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-L713F (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    L713F, V762A

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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