Skip to main content
Addgene
Showing: 3201 - 3250 of 17836 results

  4. pGS1044

    Plasmid
    #63797
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal 3xFLAG tag and a LEU2 selection marker
    Depositor
    Bjoern Sander, Monika Golas
    Article
    Lin et al Biochim Biophys Acta. 2015 Mar;1854(3):1
    Insert
    3xFLAG followed by KlLEU2
    Use
    E. coli cloning vector
    Tags
    3xFLAG
    Expression
    Mutation
    Promoter
    none
    Available since
    April 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    63797-plasmid-map-sequence-id-110117

    Enlarge

  9. BtARR1 (1-378)

    Plasmid
    #236264
    Purpose
    Bacterial expression of bovine arrestin-1 (1-378) E379Stop
    Depositor
    Vsevolod Gurevich
    Insert
    arrestin-1 (SAG Bovine)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    (1-378) E379Stop, inserted GGT codon after starti…
    Promoter
    lac
    Available since
    May 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    236264-plasmid-map-sequence-id-472931

    Enlarge

  10. BtARR1

    Plasmid
    #236262
    Purpose
    Bacterial expression of bovine arrestin-1 with inserted GGT codon after starting ATG
    Depositor
    Vsevolod Gurevich
    Insert
    arrestin-1 (SAG Bovine)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    inserted GGT codon after starting ATG
    Promoter
    lac
    Available since
    May 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    236262-plasmid-map-sequence-id-472929

    Enlarge

  11. BtARR1 R175E

    Plasmid
    #236263
    Purpose
    Bacterial expression of bovine arrestin-1 R175E with inserted GGT codon after starting ATG
    Depositor
    Vsevolod Gurevich
    Insert
    arrestin-1 (SAG Bovine)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    R175E, inserted GGT codon after starting ATG
    Promoter
    lac
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    236263-plasmid-map-sequence-id-471568

    Enlarge

  29. pJL15

    Plasmid
    #174069
    Purpose
    Expresses 43-8 zinc finger array fused to CRY2 in S. cerevisiae. Part of optogenetic system.
    Depositor
    Albert Keung
    Article
    Lee et al Cell Syst. 2021 Aug 24. pii: S2405-4712(
    Insert
    pGAL-tetO-3xNLS-43-8WT-GS-Cry2-Tadh1
    Use
    Tags
    FLAG
    Expression
    Yeast
    Mutation
    Promoter
    pGal1
    Available since
    Sept. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    174069-plasmid-map-sequence-id-342040

    Enlarge

  32. RCP83

    Plasmid
    #163466
    Purpose
    Backbone plasmid for insertion of the Saccharomyces cerevisiae promoter library and yeGFP
    Depositor
    Frank Albert
    Article
    Renganaath et al Elife. 2020 Nov 12;9. pii: 62669. doi: 1
    Inserts
    Promoter fragment (Sharon2012_1922)
    His3 minimal promoter
    yeGFP
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    163466-plasmid-map-sequence-id-322582

    Enlarge

  33. pst39-NST-clone14

    Plasmid
    #133421
    Purpose
    S. cerevisiae Nip1 (with C-terminal TEV cleavage site and strep-II tag), Sui1 and Tif5B6 (with N-terminal 7xHis tag and TEV cleavage set) coding sequences with intervening ribosome binding sites and other cis-elements for expression as a polycistron, T7 theta 10 promoter (and corresponding terminator after polycistron).
    Depositor
    Gerhard Wagner
    Insert
    NIP1/SUI1/TIF5 (NIP1, TIF5, SUI1 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    ORF for Nip1 encodes aa 4-156 (not complete ORF),…
    Promoter
    Available since
    Jan. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    133421-plasmid-map-sequence-id-267716

    Enlarge

  43. pUC57-LbNOX

    Plasmid
    #75285
    Purpose
    This plasmid contains human codon optimized sequence of LbNOX which is a water-forming NADH oxidase that can be used to increase NAD+/NADH ratio in mammalian cells.
    Depositor
    Vamsi Mootha
    Article
    Titov et al Science. 2016 Apr 8;352(6282):231-5. doi
    Insert
    LbNOX
    Use
    Tags
    C-terminal FLAG tag with linker
    Expression
    Mutation
    Human codon optimized
    Promoter
    Available since
    May 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    75285-plasmid-map-sequence-id-258257

    Enlarge

  46. pRCC-K

    Plasmid
    #81191
    Purpose
    Expression of Cas9 and gRNA cassette in S. cerevisiae; Kan Resistance
    Depositor
    Eckhard Boles
    Article
    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.
    Inserts
    Cas9
    empty gRNA cassette
    Use
    CRISPR
    Tags
    NTS
    Expression
    Yeast
    Mutation
    Please see depositor comments below. and WT - cod…
    Promoter
    ROX3 and SNP52p
    Available since
    Sept. 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    81191-plasmid-map-sequence-id-154417

    Enlarge

Showing: 3201 - 3250 of 17836 results