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Plasmid#66140PurposeExpresses the G108C mutant of Sulfolobus solfataricus PCNA1DepositorInsertDNA polymerase sliding clamp 1
UseTagsHis6 tagExpressionBacterialMutationG108CPromoterT7 promoterAvailable sinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL1632
Plasmid#62829PurposeLim Lab plasmid: AmpR + PLlacO-1::RBS(st7) gfp::Asp terminator + ColE1DepositorInsertgfp
UseSynthetic BiologyTagsst7 RBSExpressionBacterialMutationPromoterpLlacO-1Available sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pET15b+PCNA3 WT
Plasmid#66179PurposeExpresses the wild type of Sulfolobus solfataricus PCNA3DepositorInsertDNA polymerase sliding clamp 3
UseTagsHis6 tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET15b+PCNA2 WT
Plasmid#66175PurposeExpresses the wild type of Sulfolobus solfataricus PCNA2DepositorInsertDNA polymerase sliding clamp 2
UseTagsHis6 tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYI277
Plasmid#228285PurposeConstitutive EGFP expressionDepositorInsertEGFP
UseSynthetic BiologyTagsExpressionYeastMutationPromoterKpGAPDH promoterAvailable sinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDF-His_TEV_MsbA
Plasmid#191476PurposeExpresses MsbA with a TEV protease cleavable N terminal His TagDepositorInsertMsbA
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceDec. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-TRE3G-Tir
Plasmid#221326PurposeDox-inducible expression of Tir in mammalian cells by retroviral transductionDepositorInsertTir
UseRetroviralTagsHN-FLAGExpressionMutationPromoterTRE3GVAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-TRE3G-Gag
Plasmid#221325PurposeDox-inducible expression of Gag in mammalian cells by retroviral transductionDepositorInsertGag
UseRetroviralTagsFLAGExpressionMutationPromoterTRE3GVAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-FKBP-empty
Plasmid#221332PurposeProtein overexpression in bacteriaDepositorInsert6xHis-3xFKBP empty scaffold
UseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-Myc-Caspase-11_humanized-mutant
Plasmid#214316PurposeExpression of gene in mammalian cells by retroviral transductionDepositorInsertCASP4 (Casp4 Mouse)
UseRetroviralTagsMycExpressionMutationKLS288YKT_KASIHS348TPRAKAPromoterMESVAvailable sinceMay 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMS77
Plasmid#215681PurposeCas9 + guide plasmid targeting ChrIII split hygromycinR landing padDepositorInserteef-1A.1p::Cas9 + U6p::GTCCAGCGGCAGATCGGCGG
UseCRISPRTagsExpressionWormMutationPromoterAvailable sinceMarch 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-Myc-Caspase-4_R269D
Plasmid#214310PurposeExpression of gene in mammalian cells by retroviral transductionDepositorInsertCASP4 (CASP4 Human)
UseRetroviralTagsMycExpressionMutationR269DPromoterMESVAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-Myc-Caspase-4_K356D
Plasmid#214309PurposeExpression of gene in mammalian cells by retroviral transductionDepositorInsertCASP4 (CASP4 Human)
UseRetroviralTagsMycExpressionMutationK356DPromoterMESVAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC57-ITR2-H1-GFP sgRNA Target 3- eCMV- S.pyoegenes-3XFLAG-SV40NLS-ITR2
Plasmid#210739PurposeCoding for Sp Cas9 alongside Sp sgRNA targeting GFP Target 3DepositorInsertsUseCRISPRTags3xFLAG, SV40 NLS, PolyA signalExpressionMammalianMutationPromoterH1 and eCMVAvailable sinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CRISPR-Puro-sgRPL3-56
Plasmid#208979PurposepcDNA3.1 based plasmid for transient transfection of sgRNA-cas9. Containing sgRNA targeting human RPL3 (GRCh38.p14_Chr22:39312868-39312887), 56 is a number given by www.e-crisp.org.DepositorInsertsgRNA targeting human RPL3 (ENST00000216146) C-terminal, No.56 (RPL3 Human, Synthetic)
UseCRISPRTagsExpressionMammalianMutationPromoterCMV and U6 in tandemAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC57-ITR2-H1-GFP sgRNA Gollum Target 3- eCMV- SaSp-3XFLAG-SV40NLS-ITR2
Plasmid#210747PurposeCoding for SaSp Cas9 alongside Gollum sgRNA targeting GFP Target 3DepositorInsertsSaSp Cas9
Gollum sgRNA GFP Target 3
UseCRISPRTags3xFLAG, SV40 NLS, PolyA signalExpressionMammalianMutationN-terminal Sa Cas9, C-terminal Sp Cas9PromoterH1 and eCMVAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCX:γB2ΔICD-EC1-G4S-mTQ2ΔC6
Plasmid#209089PurposemTurquoise2-inserted PcdhgB2 lacking an intracellular domain (ICD)DepositorInsertPcdhgB2 (Pcdhgb2 Mouse)
UseTagsPA tagExpressionMammalianMutationPromoterChicken β-actinAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
XPO6-2Strep
Plasmid#210356PurposeExpresses XPO6 fused with 2Strep in mammalian cellsDepositorInsertXPO6 (XPO6 Human)
UseTags2StrepExpressionMammalianMutationPromoterAvailable sinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
TRAM2-2Strep
Plasmid#210374PurposeExpresses TRAM2 fused with 2Strep in mammalian cellsDepositorInsertTRAM2 (TRAM2 Human)
UseTags2StrepExpressionMammalianMutationPromoterAvailable sinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only