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Plasmid#44419DepositorInsertGGA2-VHS (GGA2 Human)
UseTagsGSTExpressionBacterialMutationAlanine 23 changed to ThreoninePromoterAvailable sinceJuly 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
iNicSnFR12
Plasmid#227142PurposeGenetically encoded fluorescent biosensor for nicotine detectionDepositorInsertiNicSnFR12
UseTags6xHis-HA and MycExpressionBacterialMutationPromoterT7Available sinceNov. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-RIPLET ΔPRY/SPRY
Plasmid#218516PurposeRIPLET lacking C-terminal PRY/SPRY domain in pcDNA3.1DepositorInsertRIPLET delta PRY/SPRY domain (RNF135 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
PABPC1_544-626_pGEX6P1
Plasmid#209790PurposeBacterial expression of PABPC1 Mlle domainDepositorInsertMlle domain of PABPC1 (PABPC1 Human)
UseTagsGSTExpressionBacterialMutationaa 544-626 onlyPromotertacAvailable sinceNov. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
OsMTI-1B Oryza sativa Metallothionein 1B
Plasmid#200295PurposeBacterial expression plasmids for production of recombinant Oryza sativa Metallothionein 1BDepositorInsertGene of metallothionein with Chiting Binding Domanin-Intein (LOC4332487 Oryza sativa)
UseTagsChiting Binding Domanin-InteinExpressionBacterialMutationPromoterAvailable sinceMay 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
PHAGE-P CMVt N-HA hE6-AP I (G20V)
Plasmid#119308Purposelentiviral vector for expression of HA-Tagged E6-AP I (G20V) in mammalian cellsDepositorInsertE6-AP I (G20V) (UBE3A Human)
UseLentiviralTagsHAExpressionMutationG20VPromoterCMVAvailable sinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
PHAGE-P CMVt N-HA hE6-AP I (C21Y)
Plasmid#119304Purposelentiviral vector for expression of HA-Tagged E6-AP I (C21Y) in mammalian cellsDepositorInsertE6-AP I (C21Y) (UBE3A Human)
UseLentiviralTagsHAExpressionMutationC21YPromoterCMVAvailable sinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
PHAGE-P CMVt N-HA hE6-AP I (AA 55-376)
Plasmid#119313Purposelentiviral vector for expression of HA-Tagged E6-AP I AA 55-376 in mammalian cellsDepositorInsertE6-AP I (UBE3A Human)
UseLentiviralTagsHAExpressionMutationOnly AA 55-376PromoterCMVAvailable sinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
PHAGE-P CMVt N-HA hE6-AP II (AA 1-87)
Plasmid#119317Purposelentiviral vector for expression of HA-Tagged E6-AP I AA 1-87 in mammalian cellsDepositorInsertE6-AP II (UBE3A Human)
UseLentiviralTagsHAExpressionMutationOnly AA 1-87PromoterCMVAvailable sinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
PHAGE-P CMVt N-HA hE6-AP I (AA 1-64)
Plasmid#119310Purposelentiviral vector for expression of HA-Tagged E6-AP I AA 1-64 in mammalian cellsDepositorInsertE6-AP I (UBE3A Human)
UseLentiviralTagsHAExpressionMutationOnly AA 1-64PromoterCMVAvailable sinceJuly 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
PHAGE-P CMVt N-HA hE6-AP I (R39H)
Plasmid#119309Purposelentiviral vector for expression of HA-Tagged E6-AP I (R39H) in mammalian cellsDepositorInsertE6-AP I (R39H) (UBE3A Human)
UseLentiviralTagsHAExpressionMutationG20VPromoterCMVAvailable sinceJuly 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
PHAGE-P CMVt N-HA hE6-AP I (AA 55-852)
Plasmid#119314Purposelentiviral vector for expression of HA-Tagged E6-AP I AA 55-852 in mammalian cellsDepositorInsertE6-AP I (UBE3A Human)
UseLentiviralTagsHAExpressionMutationOnly AA 55-852PromoterCMVAvailable sinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCSD_FKBP-C-SpCas9MT3-574-1368-NLS-3XHA-NLS-ZFPTS3-3xFLAG-2xNLS
Plasmid#107300PurposeExpresses C terminal (574-1368 aa) R1335K mutant SpCas9 fused to FKBP and ZFP-VEGFA-TS3 in mammalian cellsDepositorInsertTruncated SpCas9 (aa 574-1368) fused to FKBP
UseCRISPRTags3x Flag, 2x NLS, FKBP, SV40NLS, 3xHA, c-Myc NLS, …ExpressionMammalianMutationTruncated SpCas9 (aa 574-1368) with R1335K mutati…PromoterCMV IE94Available sinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCSD_FKBP-C-SpCas9MT3-574-1368-NLS-3XHA-NLS-ZFPTS2-3xFLAG-2xNLS
Plasmid#107299PurposeExpresses C terminal (574-1368 aa) R1335K mutant SpCas9 fused to FKBP and ZFP-VEGFA-TS2 in mammalian cellsDepositorInsertTruncated SpCas9 (aa 574-1368) fused to FKBP
UseCRISPRTags3x Flag, 2x NLS, FKBP, SV40NLS, 3xHA, c-Myc NLS, …ExpressionMammalianMutationTruncated SpCas9 (aa 574-1368) with R1335K mutati…PromoterCMV IE94Available sinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR-IAA8_no stop
Plasmid#83874PurposeGateway donor vector for IAA8 with no stop codonDepositorInsertindole-3-acetic acid inducible 8 (IAA8 Mustard Weed)
UseGateway donor vectorTagsExpressionMutationno stop codonPromoterAvailable sinceOct. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR-IAA15_no stop
Plasmid#83853PurposeGateway donor vector for IAA15 with no stop codonDepositorInsertindole-3-acetic acid inducible 15 (IAA15 Mustard Weed)
UseGateway donor vectorTagsExpressionMutationno stop codonPromoterAvailable sinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pHL2073
Plasmid#62837PurposeLim Lab plasmid: AmpR + PconShortNoHind::glmZ::Asp terminator + PLlacO-1::T710 hfq::T1 terminator + PLtetO-1m9::glmS::gfp::T1T2 terminator + ColE1DepositorInsertsglmZ leader
T710 hfq leader
gfp
UseSynthetic BiologyTagsm9::glmSExpressionBacterialMutationPromoterpConShortNoHind, pLlacO-1, and pLtetO-1Available sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL2075
Plasmid#62838PurposeLim Lab plasmid: AmpR + PconShortNoHind::glmZ::Asp terminator + PLlacO-1::T710 hfq::T1 terminator + PLtetO-1::glmS::gfp::T1T2 terminator + ColE1DepositorInsertsglmZ leader
T710 hfq leader
gfp
UseSynthetic BiologyTagsglmSExpressionBacterialMutationPromoterpConShortNoHind, pLlacO-1, and pLtetO-1Available sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL2085
Plasmid#62840PurposeLim Lab plasmid: AmpR + PconShortNoHindM8::glmZ::Asp terminator + PLlacO-1::T710 hfq::T1 terminator + PLtetO-1::glmS::gfp::T1T2 terminator + ColE1DepositorInsertsm8::glmZ leader
T710 hfq leader
gfp
UseSynthetic BiologyTagsglmSExpressionBacterialMutationPromoterpConShortNoHind, pLlacO-1, and pLtetO-1Available sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only