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pcDNA5 FRT TO GFP MPS1 K-R

Plasmid

#59819

Purpose

Allows the integration of GFP MPS1 K-R in the genome and Tet-inducible expression.

Depositor

Stephen Taylor

Article

Tighe et al J Cell Biol. 2008 Jun 16;181(6):893-901.

Insert

Mps1 (TTK Human)

Tags

EmGFP

Expression

Mammalian

Mutation

KD, catalytically inactive, D664A

Promoter

hybrid human cytomegalovirus (CMV)/TetO2 promoter

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA5 TO FRT Myc ArB K106R

Plasmid

#59807

Purpose

Allows the integration of Myc ArB K106R in the genome and Tet-inducible expression

Depositor

Stephen Taylor

Article

Girdler et al J Cell Sci. 2006 Sep 1;119(Pt 17):3664-7

Insert

Aurora B (AURKB Human)

Tags

Myc

Expression

Mammalian

Mutation

K106R, kinase mutant

Promoter

hybrid human cytomegalovirus (CMV)/TetO2 promoter

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pYTRW08K_0C5

Plasmid

#177280

Purpose

genomic integration of genes (inserted in I-SceI-site) via transposon Tn5 with CmR

Depositor

Karl-Erich Jaeger

Article

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Insert

Pcat-cat

Use

Synthetic Biology; Yeast expression, tn5 genomic …

Expression

Bacterial

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pINDUCER20-hGfi1

Plasmid

#121200

Purpose

inducible expression of hGfi1 in colorectal cancer cells

Depositor

Noah Shroyer

Article

Chen et al Mol Cancer Res. 2019 Mar;17(3):697-708.

Insert

Growth factor independent 1 transcriptional (GFI1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

P2L

Available Since

July 25, 2025

Availability

Academic Institutions and Nonprofits only

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pCDF_AtRaf1/Raf2/BSD2

Plasmid

#229511

Purpose

Expresses Arabidopsis thaliana chaperones: Raf1,Raf2,BSD2

Depositor

Laura Gunn

Article

Oh et al Mol Plant. 2024 Dec 2;17(12):1833-1849.

Inserts

Rubisco accumulation factor 1 (AT3G04550 Mustard Weed)
Rubisco accumulation factor 2 (AT5G51110 Mustard Weed)
BSD2

Expression

Bacterial

Mutation

N terminus chloroplast transit peptide truncation

Promoter

T7

Available Since

June 20, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR-AtmiR173aTS-B/c

Plasmid

#227964

Purpose

Entry plasmid with AtmiR173aTS and a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs.

Depositor

Alberto Carbonell

Article

Cisneros et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

Inserts

B/c
AtmiR173aTS

Promoter

None

Available Since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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pMDC32B-AtmiR173aTS-B/c

Plasmid

#227965

Purpose

Expression plasmid with a ccdB cassette flanked with two inverted BsaI restriction sites. For expressing syn-tasiRNAs in Arabidopsis thaliana.

Depositor

Alberto Carbonell

Article

Cisneros et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

Inserts

B/c
AtmiR173aTS

Expression

Plant

Promoter

35S and None

Available Since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR-NbmiR482aTS-B/c

Plasmid

#227966

Purpose

Entry plasmid with NbmiR482aTS and a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs.

Depositor

Alberto Carbonell

Article

Cisneros et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

Inserts

B/c
NbmiR482aTS

Promoter

None

Available Since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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pSuper DGK zeta human

Plasmid

#224575

Purpose

To downregulate the expression of human DGK zeta in human cells

Depositor

Isabel Mérida

Article

Avila-Flores et al Immunol Cell Biol. 2017 Jul;95(6):549-56

Insert

sh RNAi Diacylglycerol kinase zeta human (DGKZ Human)

Use

RNAi

Promoter

H1

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pSuper Retro GFP DGK alfa mouse

Plasmid

#224576

Purpose

Negative Control for downregulation of the expression of human DGK alfa in human cells

Depositor

Isabel Mérida

Article

Avila-Flores et al Immunol Cell Biol. 2017 Jul;95(6):549-56

Insert

sh RNAi Diacylglycerol kinase alfa mouse (Dgka Mouse)

Use

RNAi and Retroviral

Promoter

H1

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBS10-riboE-dddAI

Plasmid

#186561

Purpose

Regulated expression of the DddA immunity determinant (DddAI) in Firmicutes

Depositor

Joseph Mougous

Article

Gallagher et al Nat Microbiol. 2022 Jun;7(6):844-855. do

Insert

dddAI

Expression

Bacterial

Promoter

pSpac(hy)

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pNOC_dCas9_sgRNA Td1

Plasmid

#176257

Purpose

pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged with Nlux and sgRNA targeting the fluorescent gene tdtomato with spacer 1

Depositor

John van der Oost

Article

Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

Insert

dead spCas9

Use

CRISPR, Luciferase, and Synthetic Biology ; Expre…

Tags

luciferase

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pNOC_hfnCas12a-Nlux_crRNA NR2

Plasmid

#176245

Purpose

pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 2

Depositor

John van der Oost

Article

Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

Insert

humanized fnCas12a

Use

CRISPR, Luciferase, and Synthetic Biology ; Expre…

Tags

luciferase

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pNOC_hfnCas12a_crRNA NR2

Plasmid

#176248

Purpose

pNOC episomal plasmid harboring the humanized fnCas12a gene sequence without Nlux tag and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 2

Depositor

John van der Oost

Article

Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

Insert

humanized fnCas12a

Use

CRISPR and Synthetic Biology; Expression in micro…

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pNOC_hfnCas12a_crRNA NR1

Plasmid

#176247

Purpose

pNOC episomal plasmid harboring the humanized fnCas12a gene sequence without Nlux tag and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 1

Depositor

John van der Oost

Article

Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

Insert

humanized fnCas12a

Use

CRISPR and Synthetic Biology; Expression in micro…

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pNOC_hfnCas12a-Nlux_crRNA NR3

Plasmid

#176246

Purpose

pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 3

Depositor

John van der Oost

Article

Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

Insert

humanized fnCas12a

Use

CRISPR, Luciferase, and Synthetic Biology ; Expre…

Tags

luciferase

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pNOC_hfnCas12a_crRNA NR3

Plasmid

#176249

Purpose

pNOC episomal plasmid harboring the humanized fnCas12a gene sequence without Nlux tag and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 3

Depositor

John van der Oost

Article

Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

Insert

humanized fnCas12a

Use

CRISPR and Synthetic Biology; Expression in micro…

Available Since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

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E1b-GFP-Tol2-Gateway mmu SE-Irf2bpl K

Plasmid

#89380

Purpose

Vector for reporter assay of one mouse super-enhancer region

Depositor

Alena Shkumatava

Article

Perez-Rico et al Genome Res. 2017 Feb;27(2):259-268. doi:

Insert

Irf2bpl (Irf2bpl Mouse)

Available Since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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E1b-GFP-Tol2-Gateway mmu SE-Irf2bpl F

Plasmid

#89375

Purpose

Vector for reporter assay of one mouse super-enhancer region

Depositor

Alena Shkumatava

Article

Perez-Rico et al Genome Res. 2017 Feb;27(2):259-268. doi:

Insert

Irf2bpl (Irf2bpl Mouse)

Available Since

June 15, 2017

Availability

Academic Institutions and Nonprofits only
E1b-GFP-Tol2-Gateway mmu SE-Irf2bpl G

Plasmid

#89376

Purpose

Vector for reporter assay of one mouse super-enhancer region

Depositor

Alena Shkumatava

Article

Perez-Rico et al Genome Res. 2017 Feb;27(2):259-268. doi:

Insert

Irf2bpl (Irf2bpl Mouse)

Available Since

June 1, 2017

Availability

Academic Institutions and Nonprofits only

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