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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 3,250 results for: PAM

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Showing: 1281 - 1300 of 3250 results

  10. psgRNA Library

    Pooled Library
    #115927
    Purpose
    ~93,000 gRNAs targeting random positions along the genome of E. coli MG1655 with a NGG PAM motif. Contains ~19 targets per gene and can be used with SpdCas9 to perform pooled CRISPRi screens.
    Depositor
    David Bikard
    Article
    Cui et al Nat Commun. 2018 May 15;9(1):1912. doi:
    Expression
    Bacterial
    Species
    Escherichia coli
    Use
    CRISPR
    Available Since
    June 6, 2019
    Availability
    Academic Institutions and Nonprofits only

  12. ABE9-SpRY

    Plasmid
    #242976
    Purpose
    A-to-G base editor fused to PAM-flexible SpRY-Cas9 nickase.
    Depositor
    Marc Freichel
    Article
    Ong et al Sci Rep. 2026 Feb 20;16(1):7463. doi: 10
    Insert
    pCMV-TadA-8e (N108Q/L145T)-nSpRYCas9
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    6*His
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    Feb. 20, 2026
    Availability
    Academic Institutions and Nonprofits only
    242976-plasmid-map-sequence-id-486100

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  14. xCas9

    Plasmid
    #138565
    Purpose
    Expresses human codon-optimized expanded PAM SpCas9 variant and blasticidin resistance: EFS promoter-xCas9-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    xCas9
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    A262T, R324L, S409I, E480K, E543D, M694I, E1219V
    Promoter
    EFS
    Available Since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    138565-plasmid-map-sequence-id-274797

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  18. F11-R pEBio

    Plasmid
    #61486
    Purpose
    Expresses full-length F11R (JAM1) ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag and biotinylation sequence
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    F11R (F11R Human)
    Tags
    enzymatic biotinylation sequence and rat CD4 d3+4
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 19, 2014
    Availability
    Academic Institutions and Nonprofits only
    61486-plasmid-map-sequence-id-100152

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Showing: 1281 - 1300 of 3250 results