We narrowed to 75,815 results for: Rest

1. cBEST4

   Plasmid

   #234660

   Purpose

   Expresses cytosine base editor - spCas9n (D10A) fused to APOBEC1 and UGI, Include Golden Gate compatible cassette for sgRNA insertion

   Depositor

   Mingzi M. Zhang

   Article

   Lin et al Appl Environ Microbiol. 2025 Mar 10:e019

   Inserts

   spCas9 cytosine base editor
   Golden Gate compatible sgRNA insertion cassette
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   P3 and tcp830

   Available since

   May 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. Str-KDEL_SBP-EGFP-PLAP

   Plasmid

   #222308

   Purpose

   Synchronize the trafficking of PLAP from the ER.

   Depositor

   Franck Perez

   Article

   Fourriere et al J Cell Biol. 2019 Jul 1;218(7):2215-2231

   Insert

   Streptavidin-KDEL and PLAP fused to SBP-EGFP (ALPP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   signal peptide removed

   Promoter

   CMV

   Available since

   Feb. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMXS-IRES-Blast coUCK2 D62A

   Plasmid

   #211530

   Purpose

   Expresses codon optimized catalytic mutant UCK2

   Depositor

   Richard Possemato

   Article

   Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

   Insert

   UCK2 D62A (UCK2 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   D62A (catalytic mutant)

   Promoter

   Available since

   July 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. Q54A

   Plasmid

   #217912

   Purpose

   A plasmid encoding the hydrogel-forming protein PXP with the Q54A coil mutation

   Depositor

   Cole DeForest

   Article

   Bennett et al Adv Sci (Weinh). 2024 Mar 13:e2301708. d

   Insert

   PXP Q54A
   

   Use

   Tags

   6x His (N and C term)

   Expression

   Bacterial

   Mutation

   Glutamine at position 55 and 267 mutated to Alani…

   Promoter

   T5

   Available since

   June 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. T40A

   Plasmid

   #217911

   Purpose

   A plasmid encoding the hydrogel-forming protein PXP with the T40A coil mutation

   Depositor

   Cole DeForest

   Article

   Bennett et al Adv Sci (Weinh). 2024 Mar 13:e2301708. d

   Insert

   PXP T40A
   

   Use

   Tags

   6x His (N and C term)

   Expression

   Bacterial

   Mutation

   Threonine at position 41 and 253 mutated to Alani…

   Promoter

   T5

   Available since

   June 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. SiRES COR1C ACT-DCC-Halo

   Plasmid

   #195148

   Purpose

   image COR1C

   Depositor

   Gia Voeltz

   Article

   Striepen et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

   Insert

   CORO1C (CORO1C Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mutations to make si RNA resistant and remove act…

   Promoter

   Available since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. SiRES COR1C ACT-DCC-GFP

   Plasmid

   #195147

   Purpose

   image COR1C

   Depositor

   Gia Voeltz

   Article

   Striepen et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

   Insert

   CORO1C (CORO1C Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mutations to make si RNA resistant and remove act…

   Promoter

   Available since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCas-4/1_2_VB

   Plasmid

   #186442

   Purpose

   Encodes Cas4/1, Cas2, leader, repeat and one spacer from A. acidoterrestris (type V-B)

   Depositor

   John van der Oost

   Article

   Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

   Insert

   Cas4/1 and Cas2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCas-4/1Δ2_VB

   Plasmid

   #186443

   Purpose

   Encodes Cas4/1, leader, repeat and one spacer from A. acidoterrestris (type V-B)

   Depositor

   John van der Oost

   Article

   Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

   Insert

   Cas4/1
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX459-HypaCas9-mR2-CfANLN_sgRNA

    Plasmid

    #183879

    Purpose

    pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and cfANLN sgRNA for N-terminal tagging of anillin in canine (Canis familiaris) cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    Canis familiaris ANLN sgRNA spacer
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX459-HypaCas9-H2BC11_sgRNA

    Plasmid

    #183883

    Purpose

    pX459V2.0-HypaCas9 plasmid with H2BC11 sgRNA for C-terminal tagging of H2B histones in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    H2BC11 sgRNA spacer (H2BC11 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX459-HypaCas9-MYH10_sgRNA

    Plasmid

    #183887

    Purpose

    pX459V2.0-HypaCas9 plasmid with MYH10 sgRNA for N-terminal tagging of myosin heavy chain 10 in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    MYH10 sgRNA spacer (MYH10 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX459-HypaCas9-mR2-H2BC11_sgRNA

    Plasmid

    #183881

    Purpose

    pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and H2BC11 sgRNA for C-terminal tagging of H2B histones in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    H2BC11 sgRNA spacer (H2BC11 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX459-HypaCas9-mR2-MYH10_sgRNA

    Plasmid

    #183886

    Purpose

    pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and MYH10 sgRNA for N-terminal tagging of myosin heavy chain 10 in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    MYH10 sgRNA spacer (MYH10 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV-MNTS-1-p75

    Plasmid

    #182945

    Purpose

    To express a multi-neurotrophin made from the Human NT-3 gene. The secreted protein that can bind only all 3 TRK receptors and not p75NTR

    Depositor

    Pantelis Tsoulfas

    Article

    Urfer et al EMBO J. 1994 Dec 15. 13(24):5896-909.

    Insert

    MNTS-1-p75 (NTF3, NT3 Human, Synthetic)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter and enhancer

    Available since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV-MNTS-1

    Plasmid

    #182944

    Purpose

    To express a multi-neurotrophin made from the Human NT-3 gene. The secreted protein can bind all 3 TRK receptors and p75NTR

    Depositor

    Pantelis Tsoulfas

    Article

    Urfer et al EMBO J. 1994 Dec 15. 13(24):5896-909.

    Insert

    MNTS-1 (NTF3 Homo sapiens, Human, Synthetic)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    This is the Human NT-3 with mutations on the matu…

    Promoter

    CMV promoter and enhancer

    Available since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-sgCTRL-1

    Plasmid

    #83934

    Purpose

    Lentiviral vector expressing a control sgRNA. NOTE: This plasmid does NOT contain Cas9.

    Depositor

    David Sabatini

    Article

    Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

    Insert

    sgCTRL-1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLenti-sgCTRL-2

    Plasmid

    #83935

    Purpose

    Lentiviral vector expressing a control sgRNA. NOTE: This plasmid does NOT contain Cas9.

    Depositor

    David Sabatini

    Article

    Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

    Insert

    sgCTRL-2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLenti-sgCTRL-3

    Plasmid

    #83936

    Purpose

    Lentiviral vector expressing a control sgRNA. NOTE: This plasmid does NOT contain Cas9.

    Depositor

    David Sabatini

    Article

    Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

    Insert

    sgCTRL-3
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mTurquoise2-RnBarr1

    Plasmid

    #137795

    Purpose

    Visualization of beta-arrestin2

    Depositor

    Dorus Gadella

    Article

    Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

    Insert

    Rat beta-arrestin 1 (Arrb1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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