We narrowed to 5,292 results for: pcdna3.1

1. pcDNA3.1(+)/Puro/TagRFP-LL5b

   Plasmid

   #112828

   Purpose

   transient/stable overexpression

   Depositor

   Joshua Sanes, Tomasz Prószyński

   Article

   Proszynski et al J Cell Sci. 2013 May 15;126(Pt 10):2225-

   Insert

   LL5b (Phldb2 Mouse)
   

   Use

   Tags

   TagRFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-HaloAKAR2.2-T2A-EGFP

   Plasmid

   #216490

   Purpose

   Expression of chemigenetic PKA biosensor (low basal brightness, extremly high dynamic range) and EGFP transfection marker in mammalian cells

   Depositor

   Jin Zhang

   Article

   Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

   Insert

   HaloAKAR2.2
   

   Use

   Tags

   T2A-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-TFEB-WT-MYC (#647)

   Plasmid

   #99955

   Purpose

   mammalian expression

   Depositor

   James Brugarolas

   Article

   Vega-Rubin-de-Celis et al Autophagy. 2017 Mar 4;13(3):464-472. doi

   Insert

   TFEB (TFEB Human)
   

   Use

   Tags

   6xHis and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)

   Plasmid

   #182287

   Purpose

   Encodes codon-optimized AF mutant of M. mazei-derived pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF) & tRNACUAPyl used for amber codon suppression in mammalian cells

   Depositor

   Ivana Nikić-Spiegel

   Article

   Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

   Inserts

   codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
   PylT
   

   Use

   Tags

   nuclear export signal (NES)

   Expression

   Mammalian

   Mutation

   Y306A/Y384F (AF) double mutant of Methanosarcina …

   Promoter

   CMV and U6

   Available since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-TFEBS211A-MYC (#805)

   Plasmid

   #99957

   Purpose

   mammalian expression

   Depositor

   James Brugarolas

   Article

   Vega-Rubin-de-Celis et al Autophagy. 2017 Mar 4;13(3):464-472. doi

   Insert

   TFEB (TFEB Human)
   

   Use

   Tags

   6xHIs and Myc

   Expression

   Mammalian

   Mutation

   S211A

   Promoter

   Available since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1 human cofilin S3E

   Plasmid

   #50855

   Purpose

   expresses psuedo-phosporylated, non-activatable cofilin in mammalian cells

   Depositor

   James Bamburg

   Article

   Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

   Insert

   cofilin 1 S3E (CFL1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S3E psuedo-phosporylated, non-activatable

   Promoter

   CMV

   Available since

   Feb. 5, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

7. pcDNA3.1-CMV-mCherry-hPMCA2w/b

   Plasmid

   #111570

   Purpose

   Expresses a plasma membrane calcium pump hPMCA2w/b

   Depositor

   Baljit Khakh

   Article

   Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

   Insert

   Plasma membrane calcium pump 2, variant w/b (ATP2B2 Human)
   

   Use

   Tags

   mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-MS2-HA-HshnRNPA2-B1_Z

   Plasmid

   #148113

   Purpose

   Mammalian Expression of HshnRNPA2-B1

   Depositor

   Elisa Izaurralde

   Insert

   HshnRNPA2-B1 (HNRNPA2B1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   splicing variant of hnRNPA2 variant B1. Exon 6 an…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1 MCS-BirA(R118G)-HA

   Plasmid

   #36047

   Purpose

   To fuse your protein of interest to the N-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); HA tag

   Depositor

   Kyle Roux

   Article

   Roux et al J Cell Biol. 2012 Mar 12.

   Has Service

   Cloning Grade DNA

   Insert

   BirA(R118G)-HA
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   R118G (highly promiscuous form)

   Promoter

   Available since

   April 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1 Flag Runx1 FL (P#1790)

    Plasmid

    #14585

    Depositor

    Matija Peterlin

    Article

    Jiang et al Mol Cell Biol. 2005 Dec . 25(24):10675-8

    Insert

    Runx1 (Runx1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1/Myc-His(-)-HSulf-2

    Plasmid

    #13004

    Depositor

    Steven Rosen

    Article

    Morimoto-Tomita et al J Biol Chem. 2002 Dec 20. 277(51):49175-

    Insert

    human sulfatase 2 (SULF2 Human)
    

    Use

    Tags

    6x His and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 30, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1/Puro-CAG-JEDI-1P

    Plasmid

    #202607

    Purpose

    Genetically encoded voltage indicator (GEVI) JEDI-1P, expressed under strong mammalian promoter (CAG)

    Depositor

    Francois St-Pierre

    Article

    Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

    Insert

    JEDI-1P
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1 EYFP H148Q/I152L

    Plasmid

    #25872

    Depositor

    Peter Haggie

    Article

    Galietta et al FEBS Lett. 2001 Jun 22. 499(3):220-4.

    Insert

    EYFP H148Q/I152L
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    EYFP from pEYFP (Clontech) with H148Q and I152L m…

    Promoter

    Available since

    Aug. 16, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1 IGF1R-BirA(R118G)-HA

    Plasmid

    #109232

    Purpose

    This construct can be used in the BioID process to identify proteins that interact with the Insulin-like Growth Factor 1 Receptor (IGF1R).

    Depositor

    Victor Dzau

    Article

    Bareja et al J Biol Chem. 2018 Mar 12. pii: RA118.002

    Insert

    IGF1R (IGF1R Human)
    

    Use

    Tags

    BirA(R118G) (highly promiscuous form) and HA

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    May 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. Nrf1-Myc pcDNA3.1(+)-C-Myc

    Plasmid

    #169125

    Purpose

    expression of protein

    Depositor

    Barbara Dirac-Svejstrup

    Article

    Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

    Insert

    hNrf1 (NFE2L1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    Available since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1+-Tornado-split-nLuc

    Plasmid

    #212612

    Purpose

    For the expression of a split nLuc that produces luminescence only when circularized. To test your IRES of interest, clone into the EcoRI, BsiWI sites.

    Depositor

    Samie Jaffrey

    Article

    Unti et al Cell Chem Biol. 2023 Oct 13:S2451-9456(2

    Insert

    Tornado-CVB3-split-nLuc
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    C373T mutation in CVB3

    Promoter

    CMV

    Available since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1(+)Gα15-244-NLuc

    Plasmid

    #207154

    Purpose

    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNA15 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

    Depositor

    Dominique Schols, Tom Van Loy

    Article

    Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

    Insert

    GNA15 (GNA15 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NLuc inserted at position E(244)N

    Promoter

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. RGS-6xHis-BLIMP-1-pcDNA3.1-

    Plasmid

    #52518

    Purpose

    expresses human BLMP-1 in mammalian cells with RGS-6xHis tag at N-terminus

    Depositor

    Adam Antebi

    Article

    Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

    Insert

    Blimp1 (PRDM1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1/CIP2A(1-905) WT V5 His

    Plasmid

    #119287

    Purpose

    CIP2A WT V5 overexpression

    Depositor

    Jukka Westermarck

    Article

    Wang et al EMBO Rep. 2017 Mar;18(3):437-450. doi: 1

    Insert

    KIAA1524 (CIP2A Human)
    

    Use

    Tags

    V5 His

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Jan. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDNA3.1-spikeRBD::∆ACE2 SURF

    Plasmid

    #206957

    Purpose

    Fluorescent reporter of the interaction between SARS-CoV-2 spike and human ACE2

    Depositor

    Xiaokun Shu

    Article

    Yang et al Nat Microbiol. 2023 Jan;8(1):121-134. do

    Inserts

    sars GU280_gp02, spike glycoprotein (S SARS-CoV-2)
    ACE2 (ACE2 Human)
    

    Use

    Tags

    cSURF, mCherry, and nSURF

    Expression

    Mammalian

    Mutation

    17-615 and RBD (319-541)

    Promoter

    CMV

    Available since

    Sept. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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