We narrowed to 15,346 results for: sgRNA

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OpenCRISPR-1 sgRNA-16nt stem

Plasmid

#221566

Purpose

Express OpenCRISPR-1 sgRNA (16nt stem loop) in human cells using a U6 promoter. Plasmid also encodes a CMV-driven GFP reporter.

Depositor

Ali Madani

Article

Ruffolo et al Nature. 2025 Jul 30. doi: 10.1038/s41586

Insert

OpenCRISPR-1 sgRNA 16nt stem loop

Expression

Mammalian

Promoter

U6

Available Since

Aug. 28, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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tet-pLKO-sgRNA-puro

Plasmid

#104321

Purpose

3rd generation lentiviral plasmid for inducible expression of sgRNA; derived from tet-pLKO-puro; puromycin selection. See manual for detailed protocols.

Depositor

Nathanael Gray

Article

Huang et al eLife. 2017 Sep 19;6. pii: e26693. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

H1/TO

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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OpenCRISPR-1 sgRNA-12nt stem

Plasmid

#221567

Purpose

Express OpenCRISPR-1 sgRNA (12nt stem loop) in human cells using a U6 promoter. Plasmid also encodes a CMV-driven GFP reporter.

Depositor

Ali Madani

Article

Ruffolo et al Nature. 2025 Jul 30. doi: 10.1038/s41586

Insert

OpenCRISPR-1 sgRNA 12nt stem loop

Expression

Mammalian

Promoter

U6

Available Since

Aug. 28, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pGL3-U6-sgRNA-PGK-puromycin

Plasmid

#51133

Purpose

expresses sgRNA from the U6 promoter.

Depositor

Xingxu Huang

Article

Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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sgRNA(MS2) cloning backbone

Plasmid

#61424

Purpose

sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.

Depositor

Feng Zhang

Article

Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Dec. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV enAsCas12f-YHAM-sgRNA_v7

Plasmid

#208356

Purpose

Expression of enAsCas12f-YHAM and sgRNA_v7 in mammalian cells

Depositor

Atsushi Hoshino

Article

Hino et al Cell. 2023 Sep 22:S0092-8674(23)00963-7.

Insert

enAsCas12f-YHAM

Expression

Mammalian

Mutation

F48Y/S188H/V232A/E316M

Promoter

CMV

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pMSCV-Cas9-2A-GFP-sgRNA

Plasmid

#124889

Purpose

Contains spCas9-2A-GFP and guide RNA scaffold in single retroviral vector

Depositor

Harvey Lodish

Article

Li et al Haematologica. 2016 Jun;101(6):e216-9. d

Insert

Cas9

Use

Retroviral

Tags

2A-GFP

Promoter

MSCV

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-sasgRNACMV- mCherry-WPREpA

Plasmid

#217015

Purpose

S. aureus sgRNAs backbone along with mCherry expression

Depositor

Nadav Ahituv

Article

Matharu et al Science. 2018 Dec 13. pii: science.aau06

Insert

mCherry

Use

AAV and CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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v1em-Nterm-PE2max-U6-sgRNA

Plasmid

#198732

Purpose

AAV genome encoding N-terminal PE2max and U6 expression cassette

Depositor

David Liu

Article

Davis et al Nat Biotechnol. 2023 May 4. doi: 10.1038

Insert

Nterm PE2max-NpuN

Use

AAV

Promoter

EFS

Available Since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti SpBsmBI sgRNA Hygro

Plasmid

#62205

Purpose

An empty sgRNA expression vector for expression of sgRNA for Sp Cas9

Depositor

Rene Maehr

Article

Pham et al Methods Mol Biol. 2016;1358:43-57. doi:

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only

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AAV-U6-sgRNA-hSyn-mCherry

Plasmid

#87916

Purpose

sgRNA expressing AAV construct with a mCherry reporter driven by hSyn promoter. (replaced the GFP in pX552 from Zhang lab with mCherry)

Depositor

Alex Hewitt

Article

Hung et al Invest Ophthalmol Vis Sci. 2016 Jun 1;57

Insert

mCherry

Use

AAV and CRISPR

Expression

Mammalian

Promoter

hSyn

Available Since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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pAP215.rAAV.mU6-sgRNA.Handle.EF1a-mTagBFP2

Plasmid

#217635

Purpose

AAV backbone for sgRNA expression. Contains a Lox-flanked handle sequence for Cre-dependent PCR amplification of sgRNAs. Protospacer is cloned between BstXI and BlpI.

Depositor

Martin Kampmann

Article

Ramani et al Nat Neurosci. 2025 Aug 22. doi: 10.1038/

Insert

mU6-sgRNA, Handle sequence, EF1a-mTagBFP2

Use

AAV

Promoter

EF1alpha and mU6

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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plentiCRISPR-v2 Puro EIF2AK4/GCN2_sgRNA

Plasmid

#218528

Purpose

sgRNA targeting human EIF2AK4/GCN2

Depositor

Kivanc Birsoy

Article

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

EIF2AK4 (EIF2AK4 Human)

Use

CRISPR and Lentiviral

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pX330-NQL005-SOX2-sgRNA

Plasmid

#175553

Purpose

For transient expression of spCas9-nuclease and a sgRNA targeting the mouse SOX2 locus.

Depositor

Elzo de Wit

Article

Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:

Insert

spCas9-nuclease and sgRNA against mouse SOX2 STOP Codon

Use

Mouse Targeting

Expression

Mammalian

Available Since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pX330-NQL007-NANOG-sgRNA

Plasmid

#175555

Purpose

For transient expression of spCas9-nuclease and a sgRNA targeting the mouse NANOG locus.

Depositor

Elzo de Wit

Article

Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:

Insert

spCas9-nuclease and sgRNA against mouse NANOG STOP Codon

Use

Mouse Targeting

Expression

Mammalian

Available Since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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FASTHDR-tRNApromo-sgRNA-espCas91_1

Plasmid

#167210

Purpose

Plasmid for easy cloning of a sgRNA sequence. The Plasmid contain a tRNA promoter to facilitate the expression of any sgRNA sequence and also expresses eSpCas9(1.1)

Depositor

Oscar Perez

Article

Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pDM002-sgRNA-lenti-ZeoR

Plasmid

#200060

Purpose

Lentiviral vector for expression of sgRNA with mCherry, zeocin resistance, BlpI + BstXI cloning sites

Depositor

Britt Glaunsinger

Article

McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

Insert

Bleomycin resistance, mCherry

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mU6

Available Since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

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pPUR-mU6-sgRNA-2XPP7

Plasmid

#194504

Purpose

Empty backbone for sgRNA-2XPP7 in CRIPSR FISHer system

Depositor

Chunqing Song

Article

Lyu et al Cell Res. 2022 Nov;32(11):969-981. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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AAV sgRNA Expression Plasmid

Plasmid

#174540

Purpose

Contains restriction sites to clone in U6 promoter and sgRNA oligo. CMV-driven mCherry fluorescent marker.

Depositor

Ormond MacDougald

Article

Romanelli et al J Biol Chem. 2021 Nov 11:101402. doi: 10

Type

Empty backbone

Use

AAV

Tags

None

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti SpBsmBI sgRNA Puro

Plasmid

#62207

Purpose

An empty sgRNA expression vector for expression of sgRNA for Sp Cas9 (3rd generation lentiviral vector)

Depositor

Rene Maehr

Article

Pham et al Methods Mol Biol. 2016;1358:43-57. doi:

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

May 27, 2015

Availability

Academic Institutions and Nonprofits only

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