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pCellFree_G03 DDX5

Plasmid

#67111

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

DDX5 (DDX5 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pRSET FLIPglu-600uDelta11 Aphrodite

Plasmid

#13569

Depositor

Wolf Frommer

Article

Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

Insert

FLIPglu F16A Delta11 Aphrodite

Tags

Aphrodite and ECFP

Expression

Bacterial

Mutation

Fluorophore-derived linker shortened. F16A mutati…

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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pGWB526

Plasmid

#74868

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with T7 (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

July 14, 2016

Availability

Academic Institutions and Nonprofits only

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CP1041-ABE8e-dimer

Plasmid

#138499

Purpose

A-to-G base editor

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

ecTadA(WT)-ecTadA(8e)-nSpCas9 (CP1041)

Expression

Mammalian

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pCfB3047(gRNA XII-1)

Plasmid

#73289

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XII-1

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

guiding RNA

Use

CRISPR; Grna

Expression

Yeast

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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LbABE8e-dimer

Plasmid

#138505

Purpose

A-to-G base editor

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

ecTadA(WT)-ecTadA(8e)-dLbCas12a

Expression

Mammalian

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pRSETa-Amrose v3

Plasmid

#79539

Purpose

pRSETa (Invitrogen) plasmid expressing Amrose variant 3

Depositor

Randolph Caldwell

Article

Schoetz et al PLoS One. 2014 Sep 5;9(9):e107069. doi:

Insert

Amrose v3

Tags

6xHis, T7 epitope, and Xpress tag

Expression

Bacterial

Promoter

T7

Available Since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

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L2-AA012

Plasmid

#185751

Purpose

Vector to introduce eGFP under control of pAaEF1a into the genome of the hornwort Anthoceros agrestis via Agrobacterium mediated transformation. eGFP contains a cell membrane localization tag.

Depositor

Peter Szoevenyi

Article

Frangedakis et al New Phytol. 2021 Jun 2. doi: 10.1111/nph

Inserts

HygR2
eGFP

Tags

membrane localization tag Lti6b

Expression

Plant

Available Since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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pUPD2 AtUBQ10 (GB2478)

Plasmid

#160594

Purpose

UBQ10 promoter from A. thaliana for constitutive expression in plant systems.

Depositor

Diego Orzaez

Article

Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

Insert

AtUBQ10

Use

CRISPR and Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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TAL2295

Plasmid

#36728

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

APC-TALEN_Right (APC Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only