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pDONR221 CARD8 FIIND F405G

Plasmid

#169973

Purpose

pDONR221 for Gateway shuttling. Contains the CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (F405G)

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Gateway cloning vector

Mutation

F405G

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 CARD8 PAM F370G

Plasmid

#169979

Purpose

pDONR221 for Gateway shuttling. Contains CARD8 with a site III mutation (F370G) with sgRNA binding site mutations

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Gateway cloning vector

Mutation

PAM, F370G

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 CARD8 PAM F405G

Plasmid

#169981

Purpose

pDONR221 for Gateway shuttling. Contains CARD8 with a site III mutation (F405G) with sgRNA binding site mutations

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Gateway cloning vector

Mutation

PAM, F405G

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 NLRP1 A59P

Plasmid

#166816

Purpose

Gateway pDONR vector encoding NLRP1 with the patient mutation A59P (Herlin et al., 2020) (no stop codon)

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Use

Gateway shuttling vector

Mutation

A59P

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 NLRP1 FIIND (F1079M-L1373)

Plasmid

#166831

Purpose

Gateway pDONR vector containing NLRP1 FIIND (no stop codon)

Depositor

Daniel Bachovchin

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Use

Gateway shuttling vector

Available Since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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p1A CbbL-

Plasmid

#162693

Purpose

p1A negative control expressing inactive CbbLS (carbamylated K194M mutation) and Prk

Depositor

David Savage

Article

Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

Insert

H. neapolitanus CbbLS and S. elongatus Prk

Tags

N terminal 6x His tag on PRK

Expression

Bacterial

Mutation

CbbL K194M (inactive rubisco)

Promoter

PLtet0-1 promoter

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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p5E-161K enhancer-NR2F2-MUT

Plasmid

#138371

Purpose

Gateway compatible 5' entry clone contains human modified 161k enhancer sequence. with NR2F2 binding sites mutated

Depositor

Nathan Lawson

Article

Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

Insert

161k human enhancer with conserved NR2F2 binding sites mutated

Use

Gateway compatible

Mutation

NR2F2 sites mutated

Promoter

none

Available Since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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p5E-161K enhancer-ETS-CORE-MUT

Plasmid

#138372

Purpose

Gateway compatible 5' entry clone contains human modified 161k enhancer sequence. with ETS binding sites mutated

Depositor

Nathan Lawson

Article

Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

Insert

human 161K human enhancer with ETS bind sites mutated

Use

Gateway compatible

Mutation

ETS factor binding sites mutated

Promoter

NONE

Available Since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pZ_lacO-cP-AOX1-sLovA

Plasmid

#126741

Purpose

yeast plasmid overexpressing sLovA

Depositor

Menghao Cai

Article

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

slovA from Aspergillus terreus ATCC 20542 (LovA) codon optimized for expression in Komagataella phaffii

Tags

6xHis

Expression

Yeast

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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IF405: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-p300core

Plasmid

#121825

Purpose

pMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 fused to the p300 catalytic core for 3 or 4-component MultiSite Gateway Pro assembly.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

Sa-dCas9/p300core (open)

Use

Synthetic Biology; Pmagic gateway entry plasmid

Tags

NLS

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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