We narrowed to 13,582 results for: RING

1. pSECRETS-B

   Plasmid

   #196987

   Purpose

   For SECRETS protocol to screen for gRNA activity and specificity: BsaI cassette for gRNA expression or for cloning (x-)gRNA librarys + off-target for SECRETS counter-selection.

   Depositor

   Eric Josephs

   Article

   Herring-Nicholas et al Commun Biol. 2024 Jan 12;7(1):86. doi: 1

   Insert

   Spy gRNA cassette
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pltetO1

   Available since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. FnR8-10

   Plasmid

   #182569

   Purpose

   Human fibronectin fragment, type-III repeats 8-10, R1379A mutant

   Depositor

   Timothy Springer

   Article

   Su et al J Biol Chem. 2022 Sep;298(9):102323. doi

   Insert

   Human fibronectin fragment, type-III repeats 8-10
   

   Use

   Tags

   His and TEV cleavage site after His tag

   Expression

   Bacterial

   Mutation

   R1379A

   Promoter

   Available since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. NK83

   Plasmid

   #176610

   Purpose

   To measure Syk intensity in response to ligand clustering using DNA origami

   Depositor

   Meghan Morrissey

   Article

   Kern et al eLife. 2021 Jun 3;10. pii: 68311. doi: 1

   Insert

   NeonGreen-tSH2 Syk (Syk Synthetic, Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   n/a

   Promoter

   Available since

   May 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKM45

   Plasmid

   #154152

   Purpose

   Firefly luciferase with no promoter for negative control for luciferase assays

   Depositor

   Kevin Maringer

   Article

   Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

   Insert

   Firefly luciferase
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. ELK1 AB box pcDNA3.1/HisC

   Plasmid

   #121094

   Purpose

   Expression of the AB box fragment of ELK1

   Depositor

   Paul Herring

   Article

   Zhou et al Mol Cell Biol. 2005 Nov . 25(22):9874-85

   Insert

   ELK1 (Elk1 Mouse)
   

   Use

   Tags

   6xHis-omni-express

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTC102

   Plasmid

   #91176

   Purpose

   a derivative of pDIRECT_37J with translation initiation sequence optimized for dicots

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Engineering Reagent: 35S:TALEN
   

   Use

   TALEN

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFREE

   Plasmid

   #92050

   Purpose

   Allows inducible expression of gRNAs and Cas9 for plasmid curing.

   Depositor

   Morten Norholm

   Article

   Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

   Inserts

   Cas9
   gRNA array
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pRhamBAD and ptet

   Available since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFREE_Zeo

   Plasmid

   #92053

   Purpose

   Allows inducible expression of gRNAs and Cas9 for plasmid curing.

   Depositor

   Morten Norholm

   Article

   Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

   Inserts

   Cas9
   gRNA array
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   pRhamBAD and ptet

   Available since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEF1-alpha-V

   Plasmid

   #27290

   Depositor

   Timothy Springer

   Article

   Takagi et al Cell. 2002 Sep 6. 110(5):599-11.

   Insert

   integrin alpha V (ITGAV Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Isoform 2

   Promoter

   Available since

   March 8, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEG BacMam N term StrepII eGFP 3C

    Plasmid

    #160683

    Purpose

    Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

    Depositor

    Eric Gouaux

    Article

    Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

    Type

    Empty backbone

    Use

    Tags

    StrepII eGFP 3C

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEG BacMam

    Plasmid

    #160451

    Purpose

    vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

    Depositor

    Eric Gouaux

    Article

    Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEGFP-hsRPA70 (MSW#473)

    Plasmid

    #208067

    Purpose

    Expression of GFP-tagged hsRPA1 in human cells

    Depositor

    Marc Wold

    Article

    Haring et al J Biol Chem. 2008 Jul 4;283(27):19095-11

    Insert

    RPA1 (RPA1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pORTMAGE-Ec1

    Plasmid

    #138474

    Purpose

    Enables high-efficiency recombineering in E. coli

    Depositor

    George Church

    Article

    Wannier et al Proc Natl Acad Sci U S A. 2020 Jun 16;11

    Inserts

    CspRecT
    mutL E32K
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    E32K

    Promoter

    Available since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJRH-1328 LV EF1a-CD20 IRES-EGFP

    Plasmid

    #201918

    Purpose

    2nd generation lentiviral transfer plasmid for engineering cells to express human CD20

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

    Insert

    CD20
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFA6a-3HA-His3MX6

    Plasmid

    #41600

    Depositor

    John Pringle

    Article

    Longtine et al Yeast. 1998 Jul;14(10):953-61.

    Insert

    His3
    

    Use

    Yeast genomic targeting

    Tags

    3xHA, A. gossypii translation elongation factor 1…

    Expression

    Mutation

    Promoter

    Available since

    May 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEG BacMam C term 3C eGFP StrepII

    Plasmid

    #160686

    Purpose

    Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

    Depositor

    Eric Gouaux

    Article

    Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

    Type

    Empty backbone

    Use

    Tags

    3C eGFP StrepII

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Human ICAM-1

    Plasmid

    #8632

    Depositor

    Timothy Springer

    Article

    Staunton et al Cell 1988 Mar 25;52(6):925-33.

    Insert

    ICAM-1 (ICAM1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEGFP-hsRPA14 (MSW#503)

    Plasmid

    #208069

    Purpose

    Expression of GFP-tagged hsRPA3 in human cells

    Depositor

    Marc Wold

    Article

    Haring et al J Biol Chem. 2008 Jul 4;283(27):19095-11

    Insert

    RPA3 (RPA3 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Talin1 head EGFP fusion

    Plasmid

    #221408

    Purpose

    Mammalian expression of Talin1 head EGFP fusion for mammalian expression

    Depositor

    Timothy Springer

    Article

    Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

    Insert

    Talin1 head EGFP fusion (TLN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pORTMAGE-Pa1

    Plasmid

    #138475

    Purpose

    Enables high-efficiency recombineering in P. aeruginosa

    Depositor

    George Church

    Article

    Wannier et al Proc Natl Acad Sci U S A. 2020 Jun 16;11

    Inserts

    PapRecT
    mutL E36K
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    E36K

    Promoter

    Available since

    June 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge