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pT3TS-RfxCas13d-NLS-HA

Plasmid

#141321

Purpose

Plasmid to carry out IVT of RfxCas13d-NLS (human codon-optimized)

Depositor

Ariel Bazzini, Miguel Angel Moreno-Mateos

Article

Kushawah et al Dev Cell. 2020 Sep 28;54(6):805-817.e7.

Insert

Rfx-Cas13d (NLS-RfxCas13d-NLS)

Use

CRISPR

Tags

HA and NLS

Expression

Mutation

Promoter

Available since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pKVL2-U6gRNA_SAM(BbsI)-PGKpuroBFP-W

Plasmid

#112925

Purpose

Lentiviral vector for SAM-CRISPRa gRNA expression with puroBFP

Depositor

Kosuke Yusa

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

hU6 promoter, gRNA-SAM expression cassette

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pU6-sgGFP-NT1

Plasmid

#46914

Purpose

Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting GFP (NT1)

Depositor

Jonathan Weissman, Stanley Qi

Article

Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

Insert

sgGFP-NT1

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

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LARRYv2-T-Sapphire library 1

Pooled Library

#233213

Purpose

Barcoding pooled library

Depositor

Alejo Rodriguez-Fraticelli

Article

Singh et al Cell Stem Cell. 2025 Feb 19:S1934-5909(2

Species

Synthetic

Use

Lentiviral

Available since

March 6, 2025

Availability

Academic Institutions and Nonprofits only
FUW-OSKM

Plasmid

#20328

Purpose

Lentiviral plasmid expressing mouse Oct4, Sox2, Klf4 and cMyc for iPS cell generation

Depositor

Rudolf Jaenisch

Article

Carey et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

Insert

Oct4-P2A-Sox2-T2A-Klf4-E2A-cMyc (Myc, Sox2, Pou5f1, Klf4 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pNS35 (multiAsCas12a piggyBac)

Plasmid

#217336

Purpose

piggyBac expression of multiAsCas12a

Depositor

Luke Gilbert

Article

Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

multiAsCas12a

Use

CRISPR

Tags

6xMycNLS and HA-SV40NLS-P2A-TagBFP2

Expression

Mammalian

Mutation

R1226A/E174R/S542R/K548R

Promoter

CAG

Available since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

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pRK-ATF3

Plasmid

#26115

Depositor

Yihong Ye

Article

Wang et al Proc Natl Acad Sci U S A. 2009 Feb 17. 1

Insert

ATF3 (ATF3 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 2, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT_HaloTag7-SNAP-tag2-NLS-P2A-NLS-mTurquoise2

Plasmid

#226514

Purpose

Mamalian cell expression of nuclear HaloTag7-SNAP-tag2 and nuclear mTurquoise2

Depositor

Kai Johnsson

Article

Kühn et al bioRxiv 2024.08.28.610127

Insert

HaloTag7-SNAP-tag2-NLS-P2A-NLS-mTurquoise2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-H1-shScramble-hsynapsin-EGFP

Plasmid

#220795

Purpose

control shRNA plasmid with EGFP expression in neurons

Depositor

Pierre Vanderhaeghen

Article

Libe-Philippot et al Neuron. 2024 Oct 9:S0896-6273(24)00645-7

Insert

EGFP

Use

Tags

Expression

Mutation

Promoter

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-Jaws-KGC-GFP-ER2 (AAV8)

Viral Prep

#65014-AAV8

Purpose

Ready-to-use AAV8 particles produced from pAAV-hsyn-Jaws-KGC-GFP-ER2 (#65014). In addition to the viral particles, you will also receive purified pAAV-hsyn-Jaws-KGC-GFP-ER2 plasmid DNA. hSyn-driven Jaws-GFP expression for optogenetic neural inhibition. These AAV preparations are suitable purity for injection into animals.

Depositor

Edward Boyden

Article

Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

Promoter

Syn

Tags

GFP

Available since

Oct. 13, 2017

Availability

Academic Institutions and Nonprofits only