We narrowed to 4,411 results for: Ado;

1. pLV-hPLXNB2_PGK_Neo

   Plasmid

   #176848

   Purpose

   lentiviral vector for constitutive PLXNB2 overexpression

   Depositor

   Roland Friedel

   Article

   Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

   Insert

   hPLXNB2 (PLXNB2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   full length PLXNB2

   Promoter

   Available since

   Nov. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCJ205

   Plasmid

   #162676

   Purpose

   pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating C224A an

   Depositor

   Gregg Beckham

   Article

   Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

   Insert

   MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   C224A, C529A; codon optimized for expression in E…

   Promoter

   T7/lac

   Available since

   Feb. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCJ198

   Plasmid

   #162671

   Purpose

   pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating F495I mutation.

   Depositor

   Gregg Beckham

   Article

   Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

   Insert

   MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   F495I; codon optimized for expression in E. coli …

   Promoter

   T7/lac

   Available since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCJ196

   Plasmid

   #162669

   Purpose

   pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating catalytic mutation, S225A

   Depositor

   Gregg Beckham

   Article

   Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

   Insert

   MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   S225A; codon optimized for expression in E. coli …

   Promoter

   T7/lac

   Available since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCJ204

   Plasmid

   #162677

   Purpose

   pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating C224H and C529F mutations.

   Depositor

   Gregg Beckham

   Article

   Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

   Insert

   MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   C224H, C529F; codon optimized for expression in E…

   Promoter

   T7/lac

   Available since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCJ201

   Plasmid

   #162674

   Purpose

   pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating C224W and C529SS mutations.

   Depositor

   Gregg Beckham

   Article

   Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

   Insert

   MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   C224W, C529SS; codon optimized for expression in …

   Promoter

   T7/lac

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. JDW 249 (pTol2-Dll4-F2-ETS#2-WT-8X-E1b-EGFP)

   Plasmid

   #156417

   Purpose

   8X copies of the ETS#2 site of the murine Dll4 intronic enhancer and a minimal E1b reporter driving expression of EGFP flanked by Tol2 sites

   Depositor

   Joshua Wythe

   Article

   Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

   Insert

   murine Dll4 F2-6/F8 ETS site B/ site #2, 8X
   

   Use

   Zebrafish transgenesis

   Tags

   Expression

   Mutation

   Promoter

   E1b min pro/b-globin intron

   Available since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDF1-MCS2-EF1-Puro-RGS7-R44C+V56C

   Plasmid

   #113730

   Purpose

   Express double mutant RGS7- R44C+V65C in mamalian cells

   Depositor

   Yardena Samuels

   Article

   Qutob et al Sci Rep. 2018 Jan 12;8(1):653. doi: 10.1

   Insert

   RGS7 (RGS7 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   R44C and V56C

   Promoter

   CMV

   Available since

   Oct. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

9. PLEX-PGK-LAMP1-HaloTag

   Plasmid

   #164216

   Purpose

   pLEX lentivirus backbone expresses HaloTag tagged LAMP1 under PGK promoter

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

   Insert

   LAMP1 (Lamp1 Rat)
   

   Use

   Lentiviral

   Tags

   HaloTag

   Expression

   Mutation

   Promoter

   PGK

   Available since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. CMV-LAMP1-HaloTag

    Plasmid

    #164209

    Purpose

    Expresses HaloTag tagged LAMP1 under CMV promoter

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

    Insert

    LAMP1 (Lamp1 Rat)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-PGK-RAP2A-WT-neo

    Plasmid

    #156177

    Purpose

    Lentiviral vector for expression of RAP2A-WT, with neomycin selection

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    RAP2A (RAP2A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    wild type form

    Promoter

    human PGK

    Available since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. ARHGEF11(DHPH)-CRY2-mCherry

    Plasmid

    #89481

    Purpose

    Also known as optoGEF-RhoA, encodes for a fusion between CRY2(1-498)-mCherry and the DHPH domain of ARHGEF11

    Depositor

    Xavier Trepat Guixer

    Article

    Valon et al Nat Commun. 2017 Feb 10;8:14396. doi: 10

    Insert

    ARHGEF11(DHPH) - CRY2(1-498) - mCherry (CRY2, ARHGEF11 Mustard Weed, Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGL4.23-GW

    Plasmid

    #60323

    Purpose

    This is a modified version of the pGL4.23 vector from Promega, containing a Gateway cassette upstream of the minimal promoter. This vector can be used for for Gateway cloning of candidate enhancer elements. As negative control, use pGL4.23 without the gateway cassette (available from Promega).

    Depositor

    Jorge Ferrer

    Article

    Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

    Type

    Empty backbone

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. EF1a-mEmerald-CAPRIN1

    Plasmid

    #164218

    Purpose

    Expresses mEmerald tagged CAPRIN1 under EF-1a promoter

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

    Insert

    CAPRIN1 (CAPRIN1 Human)
    

    Use

    Lentiviral

    Tags

    mEmerald

    Expression

    Mutation

    Promoter

    EF-1a

    Available since

    June 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCJ190

    Plasmid

    #162667

    Purpose

    pET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 12 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 12 residue Gly/Ser linker
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    codon optimized for expression in E. coli K12

    Promoter

    T7/lac

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-nEFCas9

    Plasmid

    #87115

    Purpose

    SpCas9 expression AAV backbone plasmid under control of nEF promoter

    Depositor

    Juan Belmonte

    Article

    Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

    Insert

    nEF-Cas9
    

    Use

    AAV and CRISPR

    Tags

    HA-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLEX-EF1a ANXA11-mEmerald

    Plasmid

    #164210

    Purpose

    pLEX lentivirus backbone expresses mEmerald tagged ANXA11 under EF1a promoter

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

    Insert

    ANXA11 (ANXA11 Human)
    

    Use

    Lentiviral

    Tags

    mEmerald

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. Human 3' HP1a AID GFP PuroR

    Plasmid

    #127906

    Purpose

    HDR (donor) Plasmid for Inserting AID-GFP-2A-Puro into the 3' end of the Human HP1a Gene

    Depositor

    Mark Groudine

    Article

    Strom et al Elife. 2021 Jun 9;10. pii: 63972. doi: 1

    Insert

    HP1 (CBX5 Mustard Weed, Human)
    

    Use

    Donor plasmid

    Tags

    GFP, AID, PuroR

    Expression

    Mutation

    Inserting GFP AID 2A Puro into mouse 3' Hp1a

    Promoter

    Available since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pT3TS-Hf-RfxCas13d-HA

    Plasmid

    #234479

    Purpose

    Plasmid to carry out IVT of High-fidelity RfxCas13d (human codon-optimized)

    Depositor

    Miguel Angel Moreno-Mateos

    Article

    Moreno-Sanchez et al Nat Commun. 2025 Mar 16;16(1):2591. doi:

    Insert

    RfxCas13d
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Mutation

    Changed four alanine to valine in positions 134, …

    Promoter

    Available since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. PLEX-PGK-ANXA11-mCerulean

    Plasmid

    #164214

    Purpose

    pLEX lentivirus backbone expresses mCerulean tagged ANXA11 under PGK promoter

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

    Insert

    ANXA11 (ANXA11 Human)
    

    Use

    Lentiviral

    Tags

    mCerulean

    Expression

    Mutation

    Promoter

    PGK

    Available since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLVX--DmrB-DmrB-Ripk3(deltaC)-2A-mCherry-puro

    Plasmid

    #231973

    Purpose

    Tet inducible expression of DmrB-Ripk3 with mCherry to induce Ripk3 necroptosis

    Depositor

    Brian Ruffell

    Article

    Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

    Insert

    Ripk3-deltaC dimerizing construct with bi-cistronic mCherry (Ripk3 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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22. pcDNA3.1-HDAC2-H141A

    Plasmid

    #115346

    Purpose

    Expresses mouse HDAC2 cDNA with H141A point mutation

    Depositor

    Javier Gonzalez-Maeso

    Article

    Kurita et al Nat Neurosci. 2012 Sep;15(9):1245-54. do

    Insert

    Histone deacetylase 2 (Hdac2 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    H141A

    Promoter

    CMV

    Available since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET28b-SpRY-His

    Plasmid

    #179320

    Purpose

    Plasmid for bacterial expression and purification of SpRY-Cas9

    Depositor

    Miguel Angel Moreno-Mateos, Julián Cerón

    Article

    Vicencio et al Nat Commun. 2022 May 12;13(1):2601. doi:

    Insert

    Human codon-optimized Cas9 variant SpRY
    

    Use

    CRISPR

    Tags

    6xHis and SV40-NLS

    Expression

    Bacterial

    Mutation

    SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/ N13…

    Promoter

    Available since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCMV-FLuc

    Plasmid

    #170575

    Purpose

    Express firefly luciferase. Used in MLV-based SARS-CoV-2 pseudovirus assay.

    Depositor

    David Nemazee

    Article

    Rogers et al Science. 2020 Aug 21;369(6506):956-963.

    Insert

    Luc
    

    Use

    Luciferase and Retroviral

    Tags

    NA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. GFP-KIF1B beta-600-1400

    Plasmid

    #79109

    Purpose

    Mammalian expression of GFP-KIF1B beta-600-1400

    Depositor

    Susanne Schlisio

    Article

    Chen et al Cancer Discov. 2014 Apr;4(4):434-51. doi

    Insert

    GFP-KIF1B beta (600-1400 amino acids) (KIF1B Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    exon 25 missing (natural splice varient)

    Promoter

    CMV

    Available since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

26. AAV-U6-dgRNA-CAG-MPH

    Plasmid

    #106259

    Purpose

    Expresses MPH co-activator from the CAG promoter and one U6-driven dgRNA in AAV backbone

    Depositor

    Juan Belmonte

    Article

    Liao et al Cell. 2017 Dec 14;171(7):1495-1507.e15.

    Insert

    MS2-P65-HSF1 (HSF1 Human, Synthetic)
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLentiCMVblast_SLC38A2_wt

    Plasmid

    #156180

    Purpose

    CMV-driven expression of sgRNA-resistant SLC38A2 wild-type cDNA.

    Depositor

    Alec Kimmelman

    Article

    Parker et al Cancer Discov. 2020 Apr 27. pii: 2159-82

    Insert

    SLC38A2 (SLC38A2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Silent mutations to prevent targeting by sgRNA se…

    Promoter

    CMV

    Available since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCMV-GCaMP5G

    Plasmid

    #31788

    Purpose

    a single-wavelength GCaMP3-based calcium indicator with improved response. Please also see the GCaMP6s/m/f indicators.

    Depositor

    Loren Looger, GENIE Project, Douglas Kim

    Article

    Akerboom et al J Neurosci. 2012 Oct 3;32(40):13819-40.

    Insert

    GCaMP5G
    

    Use

    Tags

    6xHis, T7 epitope, and Xpress tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLenti-CMVtight-HNF1A-FLAG-Hygro

    Plasmid

    #183232

    Purpose

    Lentiviral vector; Tet-on advanced system driving the expression of tagged HNF1A

    Depositor

    Jorge Ferrer

    Article

    Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

    Insert

    HNF1 homeobox A (HNF1A Human)
    

    Use

    Lentiviral

    Tags

    AviTag and FLAG tag

    Expression

    Mutation

    Promoter

    tight TRE

    Available since

    Nov. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCJ189

    Plasmid

    #162666

    Purpose

    pET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by an 8 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by an 8 residue Gly/Ser linker
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    codon optimized for expression in E. coli K12

    Promoter

    T7/lac

    Available since

    Feb. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge