We narrowed to 2,878 results for: 623

1. pET21-pelB-LaG-3

   Plasmid

   #172740

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-3; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-3
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET21-pelB-LaG-12

   Plasmid

   #172744

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-12; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-12
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET21-pelB-LaG-14

   Plasmid

   #172745

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-14; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-14
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMV-T7-null (BPK1077)

   Plasmid

   #171128

   Purpose

   Plasmid encoding a pCMV-T7 with a 2 amino acid peptide prior to a stop codon.

   Depositor

   Benjamin Kleinstiver

   Article

   Johnson et al Mol Cell. 2022 Jun 2;82(11):2161-2166.e3

   Insert

   pCMV-T7 null expression plasmid that encodes a two amino acid peptide (Met-Asp)
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   pCMV and T7

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. SaLgCG

   Plasmid

   #164563

   Purpose

   GFP

   Depositor

   Junwei Shi

   Article

   Verma et al Nat Cell Biol. 2021 Jan 18. pii: 10.1038

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter for crRNA expression and EFS promoter…

   Available since

   May 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJUMP29[dCas9]-1A(lacZ)

   Plasmid

   #126979

   Purpose

   Level 1 vector. With lacZ as alternative cloning reporter.OriV 9 (pBBR322/ROP; medium copy number). Constitutive dCas9 in downstream secondary site.

   Depositor

   Chris French

   Article

   Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Cas9 catalytically dead

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. TIAM2_PDZ_1

   Plasmid

   #103952

   Purpose

   Protein expression and purification of TIAM2 PDZ domain

   Depositor

   Sachdev Sidhu

   Article

   Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

   Insert

   TIAM2 (TIAM2 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET23a-MmUBB(SnaBI)

   Plasmid

   #69558

   Purpose

   In vitro transcription/translation (T7)

   Depositor

   Jorge Eduardo Azevedo

   Article

   Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

   Insert

   UBB (Ubb Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   The two last codons (TATTAA; encoding the last ty…

   Promoter

   T7

   Available since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pET23a-MmUBB(Y305P)

   Plasmid

   #69557

   Purpose

   Bacterial expression; in vitro transcription/translation (T7)

   Depositor

   Jorge Eduardo Azevedo

   Article

   Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

   Insert

   UBB (Ubb Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Codon TAT encoding tyrosine 305 was changed to CC…

   Promoter

   T7

   Available since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pET14b-PITPalpha N90F

    Plasmid

    #58257

    Purpose

    bacterial expression of human PITP alpha N90F

    Depositor

    Shamshad Cockcroft

    Article

    Tilley et al Structure. 2004 Feb;12(2):317-26.

    Insert

    PITP alpha (PITPNA Human)
    

    Use

    Tags

    HIS

    Expression

    Bacterial

    Mutation

    N90F (abolishes PI binding)

    Promoter

    T7

    Available since

    July 28, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pET14b-PITPalpha K61A

    Plasmid

    #58258

    Purpose

    bacterial expression of human PITP alpha K61A

    Depositor

    Shamshad Cockcroft

    Article

    Tilley et al Structure. 2004 Feb;12(2):317-26.

    Insert

    PITP alpha (PITPNA Human)
    

    Use

    Tags

    HIS

    Expression

    Bacterial

    Mutation

    K61A (abolishes PI binding)

    Promoter

    T7

    Available since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pEBG-TFII-I L30&37P

    Plasmid

    #22166

    Depositor

    Ananda Roy

    Article

    Sacristan et al J Biol Chem. 2004 Feb 20. 279(8):7147-58

    Insert

    TFII-I (GTF2I Human)
    

    Use

    Tags

    GST and His

    Expression

    Mammalian

    Mutation

    Changed Leucine 30 to Proline and changed leucine…

    Promoter

    Available since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

13. HOXB4-FLAG-pSP65

    Plasmid

    #20990

    Depositor

    Corey Largman

    Article

    Chang et al Genes Dev. 1995 Mar 15. 9(6):663-74.

    Insert

    HOXB4 (HOXB4 Human)
    

    Use

    In vitro trans/trans

    Tags

    Expression

    Mutation

    Missing first 14 amino acids

    Promoter

    Available since

    Nov. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

14. pGEX-2TK-WW-CR

    Plasmid

    #18070

    Depositor

    Marius Sudol

    Article

    Rentschler et al Biol Chem. 1999 Apr . 380(4):431-42.

    Insert

    Dystrophin (DMD Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 29, 2008

    Availability

    Academic Institutions and Nonprofits only

15. pGEX-2TK-WW-CR-d-ZZ

    Plasmid

    #18071

    Depositor

    Marius Sudol

    Article

    Rentschler et al Biol Chem. 1999 Apr . 380(4):431-42.

    Insert

    Dystrophin (DMD Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 29, 2008

    Availability

    Academic Institutions and Nonprofits only

16. pGEX-2TK-WW

    Plasmid

    #18072

    Depositor

    Marius Sudol

    Article

    Rentschler et al Biol Chem. 1999 Apr . 380(4):431-42.

    Insert

    Dystrophin (DMD Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 29, 2008

    Availability

    Academic Institutions and Nonprofits only

17. pGEX-2TK-CR

    Plasmid

    #18073

    Depositor

    Marius Sudol

    Article

    Rentschler et al Biol Chem. 1999 Apr . 380(4):431-42.

    Insert

    Dystrophin (DMD Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 29, 2008

    Availability

    Academic Institutions and Nonprofits only

18. pGEX-2TK-CR-CT

    Plasmid

    #18074

    Depositor

    Marius Sudol

    Article

    Rentschler et al Biol Chem. 1999 Apr . 380(4):431-42.

    Insert

    Dystrophin (DMD Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 29, 2008

    Availability

    Academic Institutions and Nonprofits only

19. pGEX-2TK-CR-d-ZZ

    Plasmid

    #18075

    Depositor

    Marius Sudol

    Article

    Rentschler et al Biol Chem. 1999 Apr . 380(4):431-42.

    Insert

    Dystrophin (DMD Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 29, 2008

    Availability

    Academic Institutions and Nonprofits only

20. pGEX-2TK-WW-EF1

    Plasmid

    #18081

    Depositor

    Marius Sudol

    Article

    Rentschler et al Biol Chem. 1999 Apr . 380(4):431-42.

    Insert

    Dystrophin (DMD Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 29, 2008

    Availability

    Academic Institutions and Nonprofits only