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psiCHECK-2 3xmiR-122T

Plasmid

#35672

Depositor

Phillip Zamore

Article

Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

Insert

3 miR-122 target sites

Use

Luciferase

Promoter

HSV-TK

Available Since

April 13, 2012

Availability

Academic Institutions and Nonprofits only

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SaLgCP

Plasmid

#164562

Purpose

Puro

Depositor

Junwei Shi

Article

Verma et al Nat Cell Biol. 2021 Jan 18. pii: 10.1038

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Flag-NLS-SaCas9-P2A-PuroR

Expression

Mammalian

Promoter

U6 promoter for crRNA expression and EFS promoter…

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-SspB-EGFP-VP16-P2A-FLAG-Gal4DBD-CapN-SsrA-CapC-HA

Plasmid

#213536

Purpose

Expresses the split transcription factors in mouse liver

Depositor

Wenjing Wang

Article

Shen et al Nat Methods. 2023 Jan;20(1):112-122. doi

Insert

SspB-EGFP-VP16-P2A-FLAG-Gal4DBD-CapN-SsrA-CapC-HA-HA

Use

AAV

Tags

EGFP and HA

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pSin-5HT-1.0

Plasmid

#172882

Purpose

make sindbis virus of 5-HT sensor GRAB_5-HT1.0 and express in cells

Depositor

J. Julius Zhu

Article

Wan et al Nat Neurosci. 2021 Apr 5. pii: 10.1038/s

Insert

5HT SENSOR

Expression

Mammalian

Promoter

sp6

Available Since

Oct. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-GFP-MTn-KDEL

Plasmid

#154348

Purpose

Mammalian expression of MTn

Depositor

Wanzhong He

Article

Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

Insert

MTn

Tags

GFP

Expression

Mammalian

Available Since

July 23, 2020

Availability

Academic Institutions and Nonprofits only

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pBoBi-hALDOA

Plasmid

#210806

Purpose

For stably expression in cells

Depositor

Sheng-cai Lin

Article

Zhang et al Nature. 2017 Aug 3;548(7665):112-116. do

Insert

ALDOA (ALDOA Human)

Use

Lentiviral

Tags

HA

Promoter

CMV

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-U6-pegRNA_KCNA1-EGFP

Plasmid

#159787

Purpose

S. pyogenes pegRNA for C to A mutation at the KCNA1 site of human cells using prime editing

Depositor

Xiaolong Wang

Article

Liu et al Cell Discov. 2020 Apr 28;6:27. doi: 10.1

Insert

spacer of pegRNA targeting KCNA1 gene (KCNA1 Human)

Expression

Mammalian

Available Since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-GFP-2AFP-KDEL

Plasmid

#154349

Purpose

Mammalian expression of 2AFP

Depositor

Wanzhong He

Article

Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

Insert

2AFP

Tags

GFP

Expression

Mammalian

Available Since

July 23, 2020

Availability

Academic Institutions and Nonprofits only

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pGL3-U6-sgRNA_KCNA1-mcherry

Plasmid

#159788

Purpose

S. pyogenes sgRNA collocated with pegRNA targeting human KCNA1 gene

Depositor

Xiaolong Wang

Article

Liu et al Cell Discov. 2020 Apr 28;6:27. doi: 10.1

Insert

spacer of sgRNA targeting KCNA1 gene (KCNA1 Human)

Expression

Mammalian

Available Since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pRNA-U6-miR122 sponge

Plasmid

#35665

Depositor

Phillip Zamore

Article

Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

Insert

U6 miR-122sponge

Promoter

U6

Available Since

April 13, 2012

Availability

Academic Institutions and Nonprofits only

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pRNA-U6-TuDmiR122

Plasmid

#35669

Depositor

Phillip Zamore

Article

Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

Insert

TuD miR-122

Promoter

U6

Available Since

April 24, 2012

Availability

Academic Institutions and Nonprofits only

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pGL3-MTS-CCDB-N136-RsDddA-A-UGI-PURO

Plasmid

#205463

Purpose

A backbone containing a RsDddA fragment A for constructing mitochondria-targeted RsDdCBE expression plasmid

Depositor

Bin Shen

Article

Cheng et al Mol Ther Nucleic Acids. 2023 Sep 9;34:10

Insert

MTS; N136; ccdb; RsDddA-A; UGI

Expression

Mammalian

Available Since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-MTS-CCDB-N136-RsDddA-C-UGI-PURO

Plasmid

#205465

Purpose

A backbone containing a RsDddA fragment C for constructing mitochondria-targeted RsDdCBE expression plasmid

Depositor

Bin Shen

Article

Cheng et al Mol Ther Nucleic Acids. 2023 Sep 9;34:10

Insert

MTS; N136; ccdb; RsDddA-C; UGI

Expression

Mammalian

Available Since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pAAVsc CB6PI Gpluc7XmiR-122T

Plasmid

#35647

Depositor

Phillip Zamore

Article

Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

Insert

7 bulged miR-122 target sites

Use

AAV

Promoter

CB

Available Since

July 30, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV TBG FFluc miR122sponge

Plasmid

#35657

Depositor

Phillip Zamore

Article

Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

Insert

miR-122 sponge

Use

AAV

Promoter

TBG

Available Since

July 11, 2012

Availability

Academic Institutions and Nonprofits only

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pAAVscCBPTuDmiR122 Gpluc

Plasmid

#35654

Depositor

Phillip Zamore

Article

Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

Insert

TuD miR-122

Use

AAV

Promoter

U6

Available Since

May 7, 2012

Availability

Academic Institutions and Nonprofits only

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pSV40 7xmutmiR-122T

Plasmid

#35663

Depositor

Phillip Zamore

Article

Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

Insert

7 mutant miR-122 target sites

Use

Luciferase

Promoter

SV-40

Available Since

June 22, 2012

Availability

Academic Institutions and Nonprofits only

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pGL3-U6-pegRNA_FANCF-EGFP

Plasmid

#159785

Purpose

S. pyogenes pegRNA for C to A mutation at the FANCF site of human cells using prime editing

Depositor

Xiaolong Wang

Article

Liu et al Cell Discov. 2020 Apr 28;6:27. doi: 10.1

Insert

spacer of pegRNA targeting FANCF gene (FANCF Human)

Expression

Mammalian

Available Since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pRNA-U6-Zip let-7

Plasmid

#35670

Depositor

Phillip Zamore

Article

Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

Insert

let-7Zip

Promoter

U6

Available Since

July 11, 2012

Availability

Academic Institutions and Nonprofits only

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pRNA-U6-ZipmiR122

Plasmid

#35671

Depositor

Phillip Zamore

Article

Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

Insert

miR-122Zip

Promoter

U6

Available Since

May 7, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge