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We narrowed to 4,974 results for: codon optimized

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Showing: 1321 - 1340 of 4974 results

  1. 3xFLAG-dCas9/pMXs-neo

    Plasmid
    #51260
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Publication
    Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:
    Insert
    3xFLAG-dCas9
    Use
    CRISPR and Retroviral
    Tags
    3xFLAG tag and NLS (nuclear localization signal)
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    LTR
    Available since
    Feb. 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    51260-plasmid-map-sequence-id-77871

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  2. pHEE2E-TRI

    Plasmid
    #71288
    Purpose
    Egg cell-specific promoter-controlled expression 3×FLAG-NLS-zCas9-NLS and two sgRNAs targeting three genes: ETC2, TRY, and CPC
    Depositor
    Qi-Jun Chen
    Publication
    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10
    Inserts
    sgRNA targeting TRY and CPC genes
    sgRNA targeting ETC2 gene
    zCas9
    Use
    CRISPR; Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Zea mays codon-optimized Cas9
    Promoter
    EC1.2 enhancer fused to EC1.1 promoter and U6-26p…
    Available since
    Jan. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    71288-plasmid-map-sequence-id-166343

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  5. pRCC-N

    Plasmid
    #81192
    Purpose
    Expression of Cas9 and gRNA cassette in S. cerevisiae; Nourseothricin Resistance
    Depositor
    Eckhard Boles
    Publication
    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.
    Inserts
    Cas9
    empty gRNA cassette
    Use
    CRISPR
    Tags
    NTS
    Expression
    Yeast
    Mutation
    Please see depositor comments below. and WT - cod…
    Promoter
    ROX3 and SNP52p
    Available since
    Sept. 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    81192-plasmid-map-sequence-id-158700

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  6. pSH-EFIRES-P-AtAFB2-mCherry

    Plasmid
    #129716
    Purpose
    AAVS1 safe harbor targeting vector expressing AtAFB2-mCherry
    Depositor
    Elina Ikonen
    Publication
    Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AFB2 (AFB2 Mustard Weed)
    Use
    CRISPR and TALEN; Human safe harbor locus (a avs1…
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    codon optimized
    Promoter
    EF1a
    Available since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    129716-plasmid-map-sequence-id-254750

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  7. pU6-Universal

    Plasmid
    #52694
    Purpose
    This plasmid can be used to mutate genes in Toxoplasma gondii using the CRISPR/Cas9 system after having an appropriate protospacer cloned into it.
    Depositor
    Sebastian Lourido
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Cas9
    Toxoplasma U6 upstream region - protospacer cloning sequence - chiRNA
    Use
    CRISPR; Toxoplasma gondii
    Tags
    3X FLAG and Nuclear localization signal
    Promoter
    TgTUB1 and Toxoplasma U6
    Available since
    June 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    52694-plasmid-map-sequence-id-82051

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  8. pFC333

    Plasmid
    #87844
    Purpose
    AMA1 plasmid with Aspergillus optimized Cas9 and ble selection marker
    Depositor
    Uffe Mortensen
    Publication
    Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do
    Inserts
    Cas9
    ble (bleomycin resistance marker)
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Promoter
    Aspergillus nidulans tef1 promoter and Aspergillu…
    Available since
    May 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    87844-plasmid-map-sequence-id-174620

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  9. pDONR221-NPC1L1_STOP

    Plasmid
    #161469
    Purpose
    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.
    Depositor
    RESOLUTE Consortium, Giulio Superti-Furga
    Publication
    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-
    Insert
    NPC1L1 (NPC1L1 Human)
    Expression
    Mammalian
    Available since
    Sept. 15, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    161469-plasmid-map-sequence-id-345626

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  10. pDONR221-SLCO2B1_STOP

    Plasmid
    #161032
    Purpose
    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.
    Depositor
    RESOLUTE Consortium, Giulio Superti-Furga
    Publication
    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-
    Insert
    SLCO2B1 (SLCO2B1 Human)
    Expression
    Mammalian
    Available since
    June 21, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    161032-plasmid-map-sequence-id-334646

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  12. Tf_dr2_ph95_12_b.45_h99_dd97_4

    Plasmid
    #257199
    Purpose
    Expression construct for Tf_dr2_ph95_12_b.45_h99_dd97_4 gene, codon optimized for expression in E. coli
    Depositor
    Gregg Beckham
    Insert
    Tf_dr2_ph95_12_b.45_h99_dd97_4
    Expression
    Bacterial
    Mutation
    S19R, R46T, N48T, N49S, G62A, A65S, E72P, I87N, Q…
    Available since
    Oct. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    257199-plasmid-map-sequence-id-522658

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  13. Tf_dr3_1219ICCG4_b.46_h99_dd97_8

    Plasmid
    #257223
    Purpose
    Expression construct for Tf_dr3_1219ICCG4_b.46_h99_dd97_8 gene, codon optimized for expression in E. coli
    Depositor
    Gregg Beckham
    Insert
    Tf_dr3_1219ICCG4_b.46_h99_dd97_8
    Expression
    Bacterial
    Mutation
    R18S, S19R, E27T, N28T, R46T, E47T, N48D, N49G, Y…
    Available since
    July 29, 2026
    Availability
    Academic Institutions and Nonprofits only
    257223-plasmid-map-sequence-id-521375

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  14. Tf_dr2_1145_ICCG_b.45_h99_dd97_7

    Plasmid
    #257219
    Purpose
    Expression construct for Tf_dr2_1145_ICCG_b.45_h99_dd97_7 gene, codon optimized for expression in E. coli
    Depositor
    Gregg Beckham
    Insert
    Tf_dr2_1145_ICCG_b.45_h99_dd97_7
    Expression
    Bacterial
    Mutation
    R18S, S19R, E27T, N28T, R46T, E47S, N48T, N49G, Y…
    Available since
    July 29, 2026
    Availability
    Academic Institutions and Nonprofits only
    257219-plasmid-map-sequence-id-521371

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  15. Tf_dr3_1159ICCG1_b.45_h99_dd97_7

    Plasmid
    #257220
    Purpose
    Expression construct for Tf_dr3_1159ICCG1_b.45_h99_dd97_7 gene, codon optimized for expression in E. coli
    Depositor
    Gregg Beckham
    Insert
    Tf_dr3_1159ICCG1_b.45_h99_dd97_7
    Expression
    Bacterial
    Mutation
    R18T, S19R, S25A, E27T, N28T, R46T, E47S, N48T, N…
    Available since
    July 29, 2026
    Availability
    Academic Institutions and Nonprofits only
    257220-plasmid-map-sequence-id-521372

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  16. Tf_dr3_1219ICCG4_b.46_h99_dd97_7

    Plasmid
    #257221
    Purpose
    Expression construct for Tf_dr3_1219ICCG4_b.46_h99_dd97_7 gene, codon optimized for expression in E. coli
    Depositor
    Gregg Beckham
    Insert
    Tf_dr3_1219ICCG4_b.46_h99_dd97_7
    Expression
    Bacterial
    Mutation
    R18S, S19R, E27T, N28T, R46T, E47T, N48E, N49G, Y…
    Available since
    July 29, 2026
    Availability
    Academic Institutions and Nonprofits only
    257221-plasmid-map-sequence-id-521373

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  17. Tf_dr3_1159ICCG5_b.45_h99_dd97_8

    Plasmid
    #257222
    Purpose
    Expression construct for Tf_dr3_1159ICCG5_b.45_h99_dd97_8 gene, codon optimized for expression in E. coli
    Depositor
    Gregg Beckham
    Insert
    Tf_dr3_1159ICCG5_b.45_h99_dd97_8
    Expression
    Bacterial
    Mutation
    R18T, S19R, E26T, E27T, N28T, S35R, R46T, E47S, N…
    Available since
    July 29, 2026
    Availability
    Academic Institutions and Nonprofits only
    257222-plasmid-map-sequence-id-521374

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  18. Tf_dr2_ph90_01_b.46_h99_dd97_5

    Plasmid
    #257216
    Purpose
    Expression construct for Tf_dr2_ph90_01_b.46_h99_dd97_5 gene, codon optimized for expression in E. coli
    Depositor
    Gregg Beckham
    Insert
    Tf_dr2_ph90_01_b.46_h99_dd97_5
    Expression
    Bacterial
    Mutation
    R18T, S19R, E26T, N28T, R46T, E47S, N48T, N49G, Y…
    Available since
    July 29, 2026
    Availability
    Academic Institutions and Nonprofits only
    257216-plasmid-map-sequence-id-521368

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  19. Tf_dr3_1219ICCG1_b.46_h99_dd97_6

    Plasmid
    #257217
    Purpose
    Expression construct for Tf_dr3_1219ICCG1_b.46_h99_dd97_6 gene, codon optimized for expression in E. coli
    Depositor
    Gregg Beckham
    Insert
    Tf_dr3_1219ICCG1_b.46_h99_dd97_6
    Expression
    Bacterial
    Mutation
    R18S, S19R, E27T, N28S, R46T, E47T, N48D, N49G, Y…
    Available since
    July 29, 2026
    Availability
    Academic Institutions and Nonprofits only
    257217-plasmid-map-sequence-id-521369

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  20. Tf_dr3_1219ICCG4_b.46_h99_dd97_6

    Plasmid
    #257218
    Purpose
    Expression construct for Tf_dr3_1219ICCG4_b.46_h99_dd97_6 gene, codon optimized for expression in E. coli
    Depositor
    Gregg Beckham
    Insert
    Tf_dr3_1219ICCG4_b.46_h99_dd97_6
    Expression
    Bacterial
    Mutation
    R18S, S19R, S23A, E26Q, E27T, N28T, R46T, E47T, N…
    Available since
    July 29, 2026
    Availability
    Academic Institutions and Nonprofits only
    257218-plasmid-map-sequence-id-521370

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Showing: 1321 - 1340 of 4974 results