We narrowed to 1,719 results for: plasmid for e coli
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pLVX-RNaseHI-NES-EGFP
Plasmid#196701PurposePlasmid for stable expression of wild type bacterial RNase HI tagged with NES and EGFP that can be used to specifically degrade the DNA-RNA hybrids in the cytoplasm.DepositorInsertbacterial RNaseHI
UseLentiviralTagsExpressionMutationPromoterAvailable SinceMarch 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pST_140_LVL2 cam
Plasmid#179334PurposeNT-CRISPR plasmid for a single gRNA with spG Cas9 (near PAM-less Cas9 variant).DepositorInsertPtac tfoX, Ptet spG cas9, PJ23106 acrIIA4, Ptet gRNA with sfGFP dropout
UseCRISPRTagsExpressionMutationCas9 -> SpG Cas9 (D1135L, S1136W, G1218K, E121…PromoterAvailable SinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV_Thy1StTA
Plasmid#97411PurposeTET driver for amplified expression in cortical neuronsDepositorInserttTA
UseAAVTagsExpressionMammalianMutationPromotermouse thy1PSsAvailable SinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKD068
Plasmid#136471PurposeSSB-GFP IDL fusion expression plasmidDepositorInsertSSB-GFP (ssb E. coli)
UseTagssuperfolder GFPExpressionBacterialMutationsuperfolder GFP inserted between Phe148 and Ser149PromoterT7 promoterAvailable SinceApril 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKD072
Plasmid#136472PurposeSSB-C-term-GFP fusion expression plasmidDepositorInsertSSB-C-term-GFP (ssb E. coli)
UseTagssuperfolder GFPExpressionBacterialMutationsuperfolder GFP attached to C-terminus at Phe 178PromoterT7 promoterAvailable SinceApril 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSCL.175
Plasmid#184996PurposeExpress -Eco1 HEK3 editing ncRNA and gRNADepositorInsertEco1: HEK3 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
UseTagsSV40NLSExpressionMammalianMutationHEK3 donor, a1/a2 length extended for 27 bpPromoterH1Available SinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.177
Plasmid#184998PurposeExpress -Eco1 EMX1 editing ncRNA and gRNADepositorInsertEco1: EMX1 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
UseTagsSV40NLSExpressionMammalianMutationEMX1 donor, a1/a2 length extended for 27 bpPromoterH1Available SinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.180
Plasmid#185001PurposeExpress -Eco1 AAVS1 editing ncRNA and gRNADepositorInsertEco1: AAVS1 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
UseTagsSV40NLSExpressionMammalianMutationAAVS1 donor, a1/a2 length extended for 27 bpPromoterH1Available SinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.178
Plasmid#184999PurposeExpress -Eco1 FANCF editing ncRNA and gRNADepositorInsertEco1: FANCF targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
UseTagsSV40NLSExpressionMammalianMutationFANCF donor, a1/a2 length extended for 27 bpPromoterH1Available SinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.176
Plasmid#184997PurposeExpress -Eco1 RNF2 editing ncRNA and gRNADepositorInsertEco1: RNF2 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
UseTagsSV40NLSExpressionMammalianMutationRNF2 donor, a1/a2 length extended for 27 bpPromoterH1Available SinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.179
Plasmid#185000PurposeExpress -Eco1 HEK4 editing ncRNA and gRNADepositorInsertEco1: HEK4 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
UseTagsSV40NLSExpressionMammalianMutationHEK4 donor, a1/a2 length extended for 27 bpPromoterH1Available SinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIB184-Km
Plasmid#90195PurposeE. coli - Streptococci shuttle plasmid for gene expression in streptococci with P23 promoterDepositorTypeEmpty backboneUseTagsNoneExpressionBacterialMutationPromoterAvailable SinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRMCD28
Plasmid#167556PurposePlasmid pWSK29 encoding E. coli PhoA' for making reporter fusion proteins for topology mappingDepositorTypeEmpty backboneUseTagsPhoA'ExpressionBacterialMutationPromoterAvailable SinceMay 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRMCD70
Plasmid#167557PurposePlasmid pWSK29 encoding E. coli LacZ' for making reporter fusion proteins for topology mappingDepositorTypeEmpty backboneUseTagsLacZ'ExpressionBacterialMutationPromoterAvailable SinceMay 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIM1773-PRHIII-sfGFP-pNW33N
Plasmid#52217PurposeConstitutive sfGFP expression plasmid for use in Geobacillus and E. coliDepositorInsertPRHIII-sfGFP
UseTagsExpressionBacterialMutationPromoterPRHIIIAvailable SinceMay 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcrRNA.con
Plasmid#61285PurposeConstitutive repeat-only E. coli CRISPR arrayDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterJ23119Available SinceFeb. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGL4.26-SS-358
Plasmid#68790PurposeZF9 Op x6 -- NTRDepositorInsertsZF9 Op x6
Nitroreductase
UseTagsExpressionMammalianMutationPromoterAvailable SinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pK27Sumo_His-SUMO-nsp10-nsp16 fusion (SARS-CoV-2)
Plasmid#169191PurposeTo express nsp10-nsp16 fusion protein in E. coliDepositorInsert14His-SUMO-nsp10-GSGSGS-nsp16 (ORF1ab SARS-CoV-2, Synthetic)
UseTags14His-SUMO and Fusion protein of SARS-CoV-2 nsp10…ExpressionBacterialMutationCodon optimised for E. coliPromoterT5Available SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGST2-GST-TEV-NDP52
Plasmid#187828PurposePlasmid for the expression and purification of GST-TEV-NDP52 in E. coli Rosetta pLysS. Internal reference: SMC1586DepositorAvailable SinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only
