We narrowed to 2,568 results for: CCS

1. pCEP4-myc-EphB4

   Plasmid

   #200983

   Purpose

   Mammalian expression plasmid for myc-tagged EphB4

   Depositor

   Erik Procko

   Article

   Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

   Insert

   EphB4 (EPHB4 Human)
   

   Use

   Tags

   Extracellular c-myc epitope tag and Signal peptid…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET21a(+)_co_L-HicDH

   Plasmid

   #185453

   Purpose

   CDS of L-HicDH codon optimized for expression in Synechocystis sp. PCC 6803 in pET21a(+).

   Depositor

   Wolfgang Kroutil

   Article

   Jurkas et al Eng Microbiol. 2021 Nov 26;2(1):100008.

   Insert

   L-HicDH from Lactobacillus confusus DSM 20196
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSEVA251_ Ptrc.x.tetO2::D-HicDH

   Plasmid

   #185456

   Purpose

   CDS of D-HicDH codon optimized for expression in Synechocystis sp. PCC 6803 under the control of the synthetic promoter Ptrc.x.tetO2 and the RBS BBa_B0030 in pSEVA251 plasmid.

   Depositor

   Wolfgang Kroutil

   Article

   Jurkas et al Eng Microbiol. 2021 Nov 26;2(1):100008.

   Insert

   D-HicDH from Lactobacillus paracasei DSM 20008
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Ptrc.x.tetO2

   Available since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX459-sgPLXNB2

   Plasmid

   #86151

   Purpose

   Cas9/CRISPR plasmid for cut in human PLXNB2 gene exon 3

   Depositor

   Roland Friedel

   Article

   Huang et al Commun Biol. 2021 Jan 29;4(1):145. doi:

   Insert

   Plexin-B2 (PLXNB2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

5. pTBL405 CHYRON1 integration construct

   Plasmid

   #126442

   Purpose

   To integrate the CHYRON1 locus at HEK293site3.

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Inserts

   pU6-CHYRON1 hgRNA
   pCMV-puro
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   The SpCas9 sgRNA constant region is mutated to ma…

   Promoter

   CMV and human U6

   Available since

   June 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLX304 PCSK5 (R511C)-V5 blast

   Plasmid

   #83104

   Purpose

   Lentiviral vector for constitutive expression of human mutant PC5A (R511C) with C-terminal V5 tag

   Depositor

   Kevin Janes

   Article

   Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

   Insert

   PCSK5 (PCSK5 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Changed Arginine 511 to Cysteine

   Promoter

   Available since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

7. pLX304 PCSK5 (T288P)-V5 blast

   Plasmid

   #83101

   Purpose

   Lentiviral vector for constitutive expression of human mutant PC5A (T288P) with C-terminal V5 tag

   Depositor

   Kevin Janes

   Article

   Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

   Insert

   PCSK5 (PCSK5 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Changed Threonine 288 to Proline

   Promoter

   Available since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

8. pLX304 PCSK5 (A270T)-V5 blast

   Plasmid

   #83102

   Purpose

   Lentiviral vector for constitutive expression of human mutant PC5A (A270T) with C-terminal V5 tag

   Depositor

   Kevin Janes

   Article

   Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

   Insert

   PCSK5 (PCSK5 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Changed Alanine 270 to Threonine

   Promoter

   Available since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

9. pLX304 PCSK5 (H516P)-V5 blast

   Plasmid

   #83103

   Purpose

   Lentiviral vector for constitutive expression of human mutant PC5A (H516P) with C-terminal V5 tag

   Depositor

   Kevin Janes

   Article

   Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

   Insert

   PCSK5 (PCSK5 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Changed Histidine 516 to Proline

   Promoter

   Available since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

10. pFL-SV40-TAK1 5'-UTR

    Plasmid

    #115356

    Purpose

    firefly luciferase (Fluc) reporter containing the 5’-UTR of TAK1 mRNA

    Depositor

    Ming-Chih Lai

    Article

    Ku et al Mol Cell Biol. 2018 Dec 11;39(1). pii: M

    Insert

    TAK1 5'-UTR (MAP3K7 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDNA3-SPA mimic WT

    Plasmid

    #92350

    Purpose

    To mimic SPA RNA processing and expression

    Depositor

    Ling-Ling Chen

    Article

    Wu et al Mol Cell. 2016 Nov 3;64(3):534-548. doi:

    Insert

    SNRPN exon 9, intron9, exon10, intron10 partial partial of SPA1 (SNRPN Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLX302 PCSK5 (T288P)-V5 puro

    Plasmid

    #98709

    Purpose

    Lentiviral vector for constitutive expression of human mutant PC5A (T288P) with C-terminal V5 tag

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    PCSK5 (PCSK5 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Changed Threonine 288 to Proline

    Promoter

    Available since

    Aug. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. GIV-FLAG L249P (LP)

    Plasmid

    #86097

    Purpose

    Expression GIV in mammalian cell

    Depositor

    Pradipta Ghosh

    Article

    Aznar et al Elife. 2016 Nov 4;5. pii: e20795. doi: 1

    Insert

    GIV/Girdin (CCDC88A Human)
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    L249P

    Promoter

    CMV

    Available since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSicoR (EGFP) shPnky-2

    Plasmid

    #79142

    Purpose

    Stable expression of shRNA targeting mouse Pnky. The shRNA (and EGFP) can be excised by the addition of Cre

    Depositor

    Daniel Lim

    Article

    Ramos et al Cell Stem Cell. 2015 Apr 2;16(4):439-47.

    Insert

    shPnky-2 (Gm30731 Mouse)
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 for the shRNA and CMV for EGFP

    Available since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMIR-Report-Luc-KLF4-K2S

    Plasmid

    #62980

    Purpose

    Translational reporter for KLF4

    Depositor

    Michael Ruppert

    Article

    Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

    Insert

    KLF4 partial sequence (KLF4 Human)
    

    Use

    Luciferase

    Tags

    firefly luciferase

    Expression

    Mutation

    Fragment 446-911 of KLF4

    Promoter

    CMV

    Available since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pMIR-Report-Luc-KLF4-K3S

    Plasmid

    #62981

    Purpose

    Translational reporter for KLF4

    Depositor

    Michael Ruppert

    Article

    Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

    Insert

    KLF4 partial sequence (KLF4 Human)
    

    Use

    Luciferase

    Tags

    firefly luciferase

    Expression

    Mutation

    Fragment 911-1406 of KLF4

    Promoter

    CMV

    Available since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pMIR-Report-Luc-KLF4-K4S

    Plasmid

    #62982

    Purpose

    Translational reporter for KLF4

    Depositor

    Michael Ruppert

    Article

    Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

    Insert

    KLF4 partial sequence (KLF4 Human)
    

    Use

    Luciferase

    Tags

    firefly luciferase

    Expression

    Mutation

    Fragment 1406-1781 of KLF4

    Promoter

    CMV

    Available since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pMIR-Report-Luc-KLF4-K6S

    Plasmid

    #62983

    Purpose

    Translational reporter for KLF4

    Depositor

    Michael Ruppert

    Article

    Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

    Insert

    KLF4 partial sequence (KLF4 Human)
    

    Use

    Luciferase

    Tags

    firefly luciferase

    Expression

    Mutation

    Fragment 2086-2639 of KLF4

    Promoter

    CMV

    Available since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pET-22HT-MSI1(7-192)R53E/R61E

    Plasmid

    #60354

    Purpose

    R53E R61E double mutant of the mouse MSI1 RBD

    Depositor

    Sean Ryder

    Article

    Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

    Insert

    mouse MSI1 (7-192) (Msi1 Mouse)
    

    Use

    Tags

    His and TEV protease site

    Expression

    Bacterial

    Mutation

    R53E and R61E mutations; contains amino acids 7-1…

    Promoter

    T7

    Available since

    Nov. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pGMCK-0X750-luxAB-DAS+4

    Plasmid

    #49517

    Purpose

    Kanamycin resistant; expresses luxAB-DAS+4 under the control of a promoter containing PtetO-4C5G; integrates into the mycobacteriophage att-L5 site

    Depositor

    Dirk Schnappinger

    Article

    Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

    Insert

    P750-luxAB-DAS+4
    

    Use

    Tags

    DAS+4 (AANDENYSENYADAS)

    Expression

    Bacterial

    Mutation

    Promoter

    P750

    Available since

    Jan. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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