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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 22,638 results for: Cas9

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Showing: 1341 - 1360 of 22638 results

  1. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-EGFP

    Plasmid
    #118156
    Purpose
    Catalytically inactive Cas9 from S. pyogenes with P2A-EGFP under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Jorge Ferrer
    Insert
    KRAB-dCas9-P2A-EGFP
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Promoter
    EF1a core and U6
    Available since
    April 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    118156-plasmid-map-sequence-id-239644

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  2. Tet-inducible Luciferase reporter

    Plasmid
    #64127
    Purpose
    Photoactivatable transcription system. Luciferase reporter under the control of a tet-inducible promoter.
    Depositor
    Moritoshi Sato
    Publication
    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    luciferase
    Use
    Luciferase
    Expression
    Mammalian
    Promoter
    TetOx13-CMV
    Available since
    April 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    64127-plasmid-map-sequence-id-198432

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  4. pKMD106e - intein-Cas9(S219)

    Plasmid
    #64190
    Purpose
    Expresses intein-Cas9(S219) in mammalian cells
    Depositor
    David Liu
    Publication
    Davis et al Nat Chem Biol. 2015 May;11(5):316-8. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mammalian codon-optimized S. pyogenes intein-Cas9(S219) - NLS - 3x FLAG
    Use
    CRISPR
    Tags
    NLS - 3x FLAG (C terminal on insert)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    64190-plasmid-map-sequence-id-107549

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  8. lentiGuideFE-Puro

    Plasmid
    #170069
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element (with F+E modifications) with customizable gRNA from U6 promoter and puromycin resistance from EFS-NS promoter
    Depositor
    Neville Sanjana
    Publication
    Morris et al Science. 2023 May 19;380(6646):eadh7699.
    Insert
    Cas9 guide RNA scaffold with the F+E scaffold modification
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Oct. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    170069-plasmid-map-sequence-id-333408

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  9. PEA1-Puro

    Plasmid
    #171991
    Purpose
    Delivers all prime editing nickase components in a single, puromycin selectable plasmid
    Depositor
    Paul Thomas
    Publication
    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CbH-Cas9(H840A)-RT-T2A-Puro, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
    Expression
    Mammalian
    Promoter
    CMV for Cas9, U6 for gRNAs
    Available since
    July 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    171991-plasmid-map-sequence-id-338127

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  11. TC9pAB

    Plasmid
    #71310
    Purpose
    used as a template for in vitro transcription of Cas9 mRNA after linearizing with BbsI
    Depositor
    Karl Degenhardt
    Insert
    Cas9
    Use
    In vitro transcription
    Tags
    NLS
    Promoter
    T7
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    71310-plasmid-map-sequence-id-129410

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  14. pMB67

    Plasmid
    #47947
    Purpose
    Expresses C. elegans optimized Cas9 from the hsp-16.48 promoter
    Depositor
    Mike Boxem
    Publication
    Waaijers et al Genetics. 2013 Aug 26.
    Insert
    Cas9
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and egl-13 NLS
    Expression
    Worm
    Promoter
    hsp-16.48
    Available since
    March 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    47947-plasmid-map-sequence-id-68808

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  16. pAAVS1-TRE-Cas9- puro-polyA-CAG-rtTA

    Plasmid
    #107270
    Purpose
    Expresses Cas9 upon induction with doxicycline.
    Depositor
    Pablo Menéndez
    Publication
    Castano et al Stem Cell Res. 2017 May;21:137-140. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    hSpCas9
    rtTA
    bGH polyA-SV40polyA
    Tags
    3xFlag, Nucleoplasmin NLS, and SV40 NLS
    Expression
    Mammalian
    Promoter
    CAG and TRE
    Available since
    April 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    107270-plasmid-map-sequence-id-260738

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  17. AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR

    Plasmid
    #60226
    Purpose
    Expresses Cre recombinase and Renilla luciferase from the EFS promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.
    Depositor
    Feng Zhang
    Publication
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Inserts
    sgRNA
    Renilla luciferase
    Cre recombinase
    Use
    AAV, CRISPR, Cre/Lox, Luciferase, and Mouse Targe…
    Tags
    Cre-HA and Rluc-P2A
    Expression
    Mammalian
    Promoter
    EFS and U6
    Available since
    Oct. 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    60226-plasmid-map-sequence-id-176980

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Showing: 1341 - 1360 of 22638 results