We narrowed to 2,730 results for: GEM;

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pUPD2_miniDFR-FRT-OCSterm-FRT-miniDFR (GB4642)

Plasmid

#225430

Purpose

OCS terminator flanked by two flippase recognition target (FRT) sites located at position 112 of the SlDFR minimal promoter

Depositor

Diego Orzaez

Article

Garcia-Perez et al Plant Biotechnol J. 2025 Jan;23(1):141-1

Insert

miniDFR-FRT-OCSterm-FRT-miniDFR

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pUPD2_miniDFR-att-OCSterm-att-miniDFR (GB4641)

Plasmid

#225431

Purpose

OCS terminator flanked by two att sites of PhiC31 integrase located at position 112 of the SlDFR minimal promoter

Depositor

Diego Orzaez

Article

Garcia-Perez et al Plant Biotechnol J. 2025 Jan;23(1):141-1

Insert

miniDFR-att-OCSterm-att-miniDFR

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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MIGR1_FLAG_TAL1-long_dt tomato

Plasmid

#210639

Purpose

Overexpression of TAL1-long

Depositor

Maayan Salton

Article

Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

Insert

TAL1-long (TAL1 Human)

Use

Retroviral

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pMMK ENTR 4 CMV eGFP

Plasmid

#206272

Purpose

ENTR Vector 4 for MultiSite Gateway assembly. Encodes eGFP under the control of a CMV promoter

Depositor

Imre Berger

Article

Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

Insert

eGFP

Use

Multimate/gateway entr 4

Expression

Mammalian

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pMMK ENTR 4 CMV mTagBFP

Plasmid

#206279

Purpose

ENTR Vector 4 for MultiSite Gateway assembly. Encodes mTagBFP under the control of a CMV promoter.

Depositor

Imre Berger

Article

Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

Insert

mTagBFP

Use

Multimate/gateway entr 4

Expression

Mammalian

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsSmg6-sh(t10)RNAres_S

Plasmid

#147460

Purpose

Mammalian Expression of HsSmg6-sh(10)RNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

HsSmg6-sh(10)RNAres (SMG6 Human)

Expression

Mammalian

Mutation

two silent mutations T594C, A699G and two non sil…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsSmg7-siRNAres-shRNAres_S

Plasmid

#147463

Purpose

Mammalian Expression of HsSmg7-siRNAres-shRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

HsSmg7-siRNAres-shRNAres (SMG7 Human)

Expression

Mammalian

Mutation

three silent mutation G216A, T1185C and A1521G an…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pN1-HsSmg7-G100E-siRNAres-V5-SBP-MBP_S

Plasmid

#147472

Purpose

Mammalian Expression of HsSmg7-G100E-siRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

HsSmg7-G100E-siRNAres (SMG7 Human)

Expression

Mammalian

Mutation

three silent mutation G216A, T1185C, A1521G and o…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pN1-HsSmg7-K66ER163E-siRNAres-V5-SBP-MBP_S

Plasmid

#147473

Purpose

Mammalian Expression of HsSmg7-K66ER163E-siRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

HsSmg7-K66ER163E-siRNAres (SMG7 Human)

Expression

Mammalian

Mutation

three silent mutation G216A, T1185C, A1521G and o…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pN1-HsSmg7-siRNAres-V5-SBP-MBP_S

Plasmid

#147474

Purpose

Mammalian Expression of HsSmg7-siRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

HsSmg7-siRNAres (SMG7 Human)

Expression

Mammalian

Mutation

three silent mutation G216A, T1185C, A1521G and o…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-C1-HsUpf1_115-1129-S1107A-siRNAres_S

Plasmid

#147477

Purpose

Mammalian Expression of HsUpf1_115-1129-S1107A-siRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

HsUpf1_115-1129-S1107A-siRNAres (UPF1 Human)

Expression

Mammalian

Mutation

4 non silent mutations E167G, K282R, A442G and T1…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-C1-HsUpf1-S1107A-siRNAres_S

Plasmid

#147478

Purpose

Mammalian Expression of HsUpf1-S1107A-siRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

HsUpf1-S1107A-siRNAres (UPF1 Human)

Expression

Mammalian

Mutation

5 non silent mutations A69S, E167G, K282R, A442G…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-TBG-Cre-sgArid1a

Plasmid

#192165

Purpose

AAV-TBG-Cre-sgArid1a

Depositor

Sidi Chen

Article

Wang et al Cancer Discov. 2020 Dec;10(12):1912-1933

Type

Empty backbone

Use

AAV

Mutation

NA

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-TBG-Cre-sgKmt2d

Plasmid

#192164

Purpose

AAV-TBG-Cre-sgKmt2d

Depositor

Sidi Chen

Article

Wang et al Cancer Discov. 2020 Dec;10(12):1912-1933

Type

Empty backbone

Use

AAV

Mutation

NA

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pN1-HsSmg5-G120E-siRNAres-V5-SBP-MBP_S

Plasmid

#147470

Purpose

Mammalian Expression of HsSmg5-G120E-siRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

HsSmg5-G120E-siRNAres (SMG5 Human)

Expression

Mammalian

Mutation

one silent mutation C969T compared to the sequenc…

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCEP4-HA-Flag-natT1R2 d835

Plasmid

#113959

Purpose

mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant

Depositor

Erik Procko

Article

Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

Insert

T1R2 (TAS1R2 Human)

Tags

FLAG and Signal/leader sequence from influenza he…

Expression

Mammalian

Mutation

residues 22-834

Promoter

cmv

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-Akt2(K14A/R25E)-Halo-PS

Plasmid

#86632

Purpose

Expresses eGFP-Akt2-HaloTag-PS fusion (cytosolic tandem)

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt2(K14A/R25E)-Halo-PS (AKT2 )

Expression

Mammalian

Available Since

March 8, 2017

Availability

Academic Institutions and Nonprofits only

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pFBD-Akt13C(WT)

Plasmid

#86576

Purpose

Expresses cleavable human full-length Akt1(WT)

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt1 (AKT1 Human)

Tags

10xHis

Expression

Insect

Mutation

3C cleavage site inserted at pos. 130

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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HM3C NaVSp1 pET24b

Plasmid

#128088

Purpose

Bacterial expression vector for NaVSp1 bacterial sodium channel. It expresses for HIStag-MBP-3C-channel protein

Depositor

Dan Minor

Article

Shaya et al Proc Natl Acad Sci U S A. 2011 Jul 26;10

Insert

NaVSp1p

Tags

3C Cleavage, His Tag, and MBP

Expression

Bacterial

Availability

Academic Institutions and Nonprofits only

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pX333

Plasmid

#64073

Purpose

Vector for tandem expression of two sgRNAs from two independent U6 promoters. Cas9 is expressed by Cbh promoter.

Depositor

Andrea Ventura

Article

Maddalo et al Nature. 2014 Dec 18;516(7531):423-7. doi

Has Service

Cloning Grade DNA

Type

Empty backbone

Tags

3XFLAG-Cas9

Expression

Mammalian

Promoter

CBh; U6

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only

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