We narrowed to 4,698 results for: pcr
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Plasmid#188332PurposePCR template for repair templates to tag a gene with a mKate2 and mIAA7 AID degron using homology-directed repair for C. elegans.DepositorInsertmIAA7::mKate2::Linker
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJJS003
Plasmid#188326PurposePCR template for repair templates to tag a gene with a mCherry and mIAA7 AID degron using homology-directed repair for C. elegans.DepositorInsertmIAA7::mCherry::Linker
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTopo pLox hPGK-Puro pA pLox
Plasmid#171048PurposeEmpty donor template for CRISPR/Cas9 targeting of TERT RE'sDepositorTypeEmpty backboneUseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-blast-eGFP
Plasmid#179781PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInserteGFP
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJJS010
Plasmid#188333PurposePCR template for repair templates to tag a gene with a mKate2 and mIAA7 AID degron using homology-directed repair for C. elegans.DepositorInsertLinker::mKate2::mIAA7
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJJS004
Plasmid#188327PurposePCR template for repair templates to tag a gene with a mCherry and mIAA7 AID degron using homology-directed repair for C. elegans.DepositorInsertLinker::mCherry::mIAA7
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-TOPO-kcnn2
Plasmid#164958PurposeZebrafish kcnn2 gene CDSDepositorInsertkcnn2 (kcnn2 Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-BC-EF1a-Puro
Plasmid#127170PurposePCR template for EF1a-puro resistance cassetteDepositorInsertNone
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-hygro-10Myc
Plasmid#179814PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsert10xMyc
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-hygro-10FLAG
Plasmid#179815PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsert10xFLAG
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFA NuiHA-loxP-nat-CUP1p
Plasmid#131170PurposePCR template for genomic N-terminal Nui-HA tag, nat-CUP1p flanking loxP sites allow expression by the native promoter after CRE-recombinase expression, nat marker confers resistance to NourseothricinDepositorTypeEmpty backboneUseTagsNui-HAExpressionYeastMutationPromoterAvailable sinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-BC-CMV-Puro
Plasmid#127169PurposePCR template for CMV-puro resistance cassetteDepositorInsertNone
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEntry Tshld-GFP
Plasmid#53211PurposepEntry Tshld-GFP is a product of BP recombination reaction between attB flanked Tshld system-encoded PCR product and pDonor 201 plasmid.DepositorInsertTshld-GFP
UseTagsEGFP, Flag, and HAExpressionMutationPromoternoneAvailable sinceMay 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-blast-mCherry
Plasmid#179789PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertmCherry
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCY 3080-01
Plasmid#36226DepositorInsertyEmCitrine
UseYeast cassette for pcr and homologous recombinati…TagsyEpolylinker-yEmCitrine-kanMXExpressionMutationPromoterAvailable sinceAug. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMOTag-M9-4H
Plasmid#106379PurposeCan be used as a PCR template to amplify the M9-HA point mutant of glmS fragment for gene specific tagging that only allows tagging but not regulation. Acts as a control for glmS based gene regulationDepositorInsertGlmS with M9 mutation
UseTagsExpressionMutationPromoterAvailable sinceMarch 2, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pgRNA-guideless
Plasmid#129464PurposeDerived from pSCB2-sgRNA. 20 nucleotide binding sequence of sgRNA removed by inverse PCR. Used as a negative control.DepositorInsertGuideless gRNA backbone
UseCRISPRTagsExpressionBacterialMutationPromoterBBa_J23119 (SpeI)Available sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPOTv6-blast-3Myc:HaloTag:3Myc
Plasmid#181943PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertHaloTag
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCfB8792
Plasmid#126910PurposeTemplate for PCR amplification of ADE2 gRNA biobrick for targeting Saccharomyces cerevisiae ADE2 geneDepositorInsertADE2 gRNA
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-blast-mScarlet
Plasmid#179818PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertmScarlet
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only