We narrowed to 6,459 results for: plasmid dna
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Plasmid#204701PurposeThis plasmid allows for inducible expression of engineered BRD4-p300(I) fusion tagged with mNeonGreen, in mammalian cells.DepositorUseTagsmNeonGreenExpressionMammalianMutationdeletion of intrinsically disordered regions of p…PromoterCMVAvailable sinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only
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pcDNA3.1(+)/(CAA)12 + (AGAGAG)7 5′ leader GUG-nLuc-3XFLAG
Plasmid#127327PurposeExpress 3xFLAG tagged nanoLuciferase (nLuc) from GUG start codon from mRNA with (AGAGAG)7 repeats in 5' UTRDepositorInsertnanoLuciferase
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/(CAA)12 + (AGAGAG)7 5′ leader AUG-nLuc-3XFLAG
Plasmid#127326PurposeExpress 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon from mRNA with (AGAGAG)7 repeats in 5' UTRDepositorInsertnanoLuciferase
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJune 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 CMV 3xFG eIF4G1 +MIC
Plasmid#158776PurposepcDNA5 transfection plasmid for the exogenous expression of N-terminal 3xFlag-tagged eIF4G1 isoform including the microexon.DepositorInserteIF4G1 (with microexon)
UseTags3xFlagExpressionMammalianMutationPromoterAvailable sinceAug. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 frt/to N-BioTAP-C-BRD4-NUT
Plasmid#171630PurposeExpresses BioTAP-tagged BRD4-NUT in mammalian cells. Can be inserted into a FRT site by co-transfection with a plasmid encoding Flp recombinaase.DepositorUseTagsBioTAPExpressionMammalianMutationPromoterAvailable sinceAug. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 V5 His-TOPO-GluCl optalpha-mYFP L9'F (Opt α-GluClv2.0)
Plasmid#47387Purposeallow pharmacologically induced silencing of electrical activity in CNS neurons upon exposure to the anthelmintic drug ivermectinDepositorInsertglutamate-gated chloride Channels
UseTagsExpressionMammalianMutationLeu9 prime mutated to F (Phenylalanine in M3-M4 l…PromoterCMVAvailable sinceAug. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
PCDNA3-FlipGFP(TEV cleavage seq) T2A mCherry
Plasmid#124429PurposeExpresses FlipGFP (TEV cleavage sequence) and T2A mCherry in mammalian cellsDepositorInsertFlipGFP(TEV cleavage seq) T2A mCherry
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-PS11-scFvFc-CD28-gp41 (706-713)
Plasmid#60606PurposeMammalian Display vectorDepositorInsertPS11-scFvFc-CD28-gp41 (706-713) (CD28 Human)
UseTagsC9ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
(344) pcDNA3.1-HA-nGFP-(EAAAK)4-OGA(544-706)
Plasmid#168097PurposeHA- tagged GFP nanobody fused to human OGA stalk domain residues (544-706). Used in combination with myc-OGA(1-400) to create split-OGA.DepositorInsertHA-nGFP-(EAAAK)4-OGA(544-706)
UseTagsHA-TagExpressionMammalianMutationPromoterT7/CMVAvailable sinceMay 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
(367) pcDNA3.1-HA-nUbc-(EAAAK)4-OGA(544-706)
Plasmid#168099PurposeHA- tagged Ubc6E nanobody fused to human OGA stalk domain residues (544-706). Used in combination with myc-OGA(1-400) to create split-OGADepositorInsertHA-nUbc-OGA(544-706)
UseTagsHA-tagExpressionMammalianMutationOGA truncated to contain residues 544-706PromoterT7/CMVAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-N_DNAAF10:1-357
Plasmid#211138PurposeMammalian expression of full-length human DNAAF10. N-terminal HiBiT tag.DepositorInsertDNAAF10:M1-I357 (WDR92 Human)
UseTagsMVSGWRLFKKISGSSGGSSGExpressionMammalianMutationwild typePromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-C_DNAAF10:1-357
Plasmid#211179PurposeMammalian expression of full-length human DNAAF10. C-terminal HiBiT tag.DepositorInsertDNAAF10:M1-I357 (WDR92 Human)
UseTagsGSSGGSSGVSGWRLFKKISExpressionMammalianMutationwild typePromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-N_DNAI4:1-848
Plasmid#211258PurposeMammalian expression of full-length human DNAI4. N-terminal HiBiT tag.DepositorInsertDNAI4:M1-A848 (WDR78 Human)
UseTagsMVSGWRLFKKISGSSGGSSGExpressionMammalianMutationG16A natural variant VAR_057635. C33W natural v…PromoterCMVAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-C_DNAI1:1-699
Plasmid#211275PurposeMammalian expression of full-length human DNAI1. C-terminal HiBiT tag.DepositorInsertDNAI1:M1-T699 (DNAI1 Human)
UseTagsGSSGGSSGVSGWRLFKKISExpressionMammalianMutationA8S Y202C P495Q annotated sequence conflictsPromoterCMVAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-N_DNAI1:1-699
Plasmid#211201PurposeMammalian expression of full-length human DNAI1. N-terminal HiBiT tag.DepositorInsertDNAI1:M1-T699 (DNAI1 Human)
UseTagsMVSGWRLFKKISGSSGGSSGExpressionMammalianMutationA8S Y202C P495Q annotated sequence conflictsPromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 rtTA BirA eIF4G +MIC
Plasmid#158790PurposepcDNA5 based transfection plasmid for the exogenous expression of N-terminal BirA-Flag-tagged eIF4G1 including the microexon for generation of N2A FlpIn cell lines for BioIDDepositorInserteIF4G1 (with microexon)
UseTags3xFlag and BirA*ExpressionMammalianMutationPromoterminiCMVAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1D/mEGFP + CrPV IGR IRES-nLuc-3XFLAG-CL1PEST
Plasmid#127332PurposeExpress 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from CrPV IRESDepositorInsertnanoLuciferase
UseTags3xFLAG and CL1/PESTExpressionMammalianMutationPromoterCMVAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
(271) pcDNA3.1-HA-nEPEA-(EAAAK)3-OGA(544-706)
Plasmid#168102PurposeHA- tagged EPEA nanobody fused to human OGA stalk domain residues (544-706). Used in combination with myc-OGA(1-400) to create split-OGADepositorInsertHA-nEPEA-(EAAAK)3-OGA(544-706)
UseTagsHA-tagExpressionMammalianMutationOGA truncated to contain residues 544-706PromoterT7/CMVAvailable sinceMay 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 T21R-F8.2(S54L+T127A)-T2A-mCherry
Plasmid#225588PurposeMammalian expression vector for TRIM21 RING-F8.2(S54L+T127A) anti-tau degrader with a self-cleaving T2A-mCherry fluorescent expression reporterDepositorInsertT21R-F8.2(S54L+T127A)-T2A-mCherry
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4TO T21R-F8.2 (S54L+T127A)-T2A-mCherry
Plasmid#225584PurposeMammalian expression vector for Doxycycline inducible expression of TRIM21 RING-F8.2(S54L+T127A) anti-tau degrader with a self-cleaving T2A-mCherry fluorescent expression reporterDepositorInsertT21R-F8.2 (S54L+T127A)-T2A-mCherry
UseTagsmCherryExpressionMammalianMutationT21R-F8.2 (S54L+T127A)PromoterCMVAvailable sinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only