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pBCV

Plasmid

#62659

Purpose

Mammalian expression of FLPo recombinase from the broadly active CAG promoter/enhancer

Depositor

Masato Ohtsuka

Article

Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

Insert

FLPo

Expression

Mammalian

Available Since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pVenus_Lys-less-Cdc20 (1620)

Plasmid

#39849

Depositor

Jonathon Pines

Article

Nilsson et al Nat Cell Biol. 2008 Dec . 10(12):1411-20

Insert

Cdc20 (CDC20 Human)

Tags

Venus

Expression

Mammalian

Mutation

Lys-less = all Lys converted to Arg

Available Since

Oct. 31, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3.2/V5 mmu-mir-153

Plasmid

#26330

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

mmu-mir-153 (Mir153 Mouse)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
pJL1-TTlight-4xDQNAT

Plasmid

#128394

Purpose

In vitro expression of tetanus toxin light chain with C terminal 4xDQNAT glycosylation sites

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

Insert

Tetanus toxin light chain domain

Tags

4xDQNAT glycosylation tag and 6xHis tag

Expression

Bacterial

Mutation

Inactivating E234A mutation

Promoter

T7

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/V5 mmu-mir-138-1

Plasmid

#26328

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

mmu-mir-138-1 (Mir138-1 Mouse)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3.2/V5 hsa-mir-483

Plasmid

#26315

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

hsa-mir-483 (MIR483 Human)

Expression

Mammalian

Available Since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only
BII-ChBtW-eSpCas9

Plasmid

#133370

Purpose

Piggybac vector for constitutive expression

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

eSpCas9

Tags

Flag

Expression

Mammalian

Mutation

eSpCas9 mutations

Promoter

CMV enhancer and hEf1a (CpG free)

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pG.Lantern-SM50-GFP

Plasmid

#135593

Purpose

Expression of SM50 Promoter-GFP mRNA for microinjection to visualize SM50 promoter activity in S. purpuratus

Depositor

Koji Akasaka, Mamiko Yajima

Article

Yajima et al Dev Genes Evol. 2007 Apr;217(4):331-6. d

Insert

SM50 promoter (SM50 S. purpuratus)

Use

In vitro transcription (mrna synthesis)

Tags

Green Lantern GFP

Promoter

CMV

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pTrc99S-ssDsbA-PD-4xDQNAT

Plasmid

#128399

Purpose

Periplasmic expression of H. influenzae protein D with C terminal 4xDQNAT glycosylation sites in E. coli

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

Insert

H. influenzae protein D

Tags

4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

Expression

Bacterial

Promoter

trc

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pTrc99S-ssDsbA-PorA-4xDQNAT

Plasmid

#128400

Purpose

Periplasmic expression of N. meningitidis PorA with C terminal 4xDQNAT glycosylation sites in E. coli

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

Insert

N. meningitidis PorA

Tags

4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

Expression

Bacterial

Promoter

trc

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221-RAP1

Plasmid

#84525

Purpose

This plasmid can be used as a donor for subcloning into Gateway destination vectors.

Depositor

Jorrit Enserink

Article

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Use

Gateway donor plasmid

Mutation

no stop codon

Promoter

none

Available Since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pENTR4_FLAG/HA_EWSR1_His6

Plasmid

#26364

Depositor

Thomas Tuschl

Article

Spitzer et al Genes Chromosomes Cancer. 2011 May . 50(

Insert

EWSR1 (EWSR1 Human)

Use

Entry vector

Tags

FLAG/HA and His6

Mutation

delta 657 (stop codon)

Available Since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3.2/V5 mmu-mir-139

Plasmid

#26329

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

mmu-mir-139 (Mir139 Mouse)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3.2/V5 mmu-mir-1224

Plasmid

#26337

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

mmu-mir-1224 (Mir1224 Mouse)

Expression

Mammalian

Available Since

Oct. 19, 2010

Availability

Academic Institutions and Nonprofits only
pBER

Plasmid

#62662

Purpose

Donor vector for tdTomato knock-in via landing pad

Depositor

Masato Ohtsuka

Article

Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

Inserts

tdTomato
hygromycin

Use

Cre/Lox

Available Since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pBGX

Plasmid

#62666

Purpose

Donor vector for Dre knock-in via landing pad

Depositor

Masato Ohtsuka

Article

Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

Insert

Dre

Use

Cre/Lox

Available Since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/V5 mmu-mir-217

Plasmid

#26333

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

mmu-mir-217 (Mir217 Mouse)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
pDONR221-rap1-K651R

Plasmid

#84528

Purpose

This plasmid can be used as a donor for subcloning into Gateway destination vectors.

Depositor

Jorrit Enserink

Article

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Use

Gateway donor plasmid

Mutation

K651R

Promoter

none

Available Since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pBGO

Plasmid

#62671

Purpose

Donor vector for mFTH1 knock-in via landing pad

Depositor

Masato Ohtsuka

Article

Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

Insert

FTH1

Use

Cre/Lox

Tags

HA

Expression

Mammalian

Available Since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/V5 mmu-mir-1197

Plasmid

#26339

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

mmu-mir-1197 (Mir1197 Mouse)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3.2/V5 mmu-mir-216a

Plasmid

#26332

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

mmu-mir-216a (Mir216a Mouse)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
RCAS (B) Eya2 (CT#665)

Plasmid

#13877

Depositor

Cliff Tabin

Article

Heanue et al Genes Dev. 1999 Dec 15. 13(24):3231-43.

Insert

Eya2 (EYA2 Chicken)

Use

Retroviral; Avian expression

Mutation

*

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3.2/V5 mmu-mir-1964

Plasmid

#26343

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

mmu-mir-1964 (Mir1964 Mouse)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3.2/V5 mmu-mir-224

Plasmid

#26335

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

mmu-mir-224 (Mir224 Mouse)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3.2/V5 hsa-mir-888

Plasmid

#26316

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

hsa-mir-888 (MIR888 Human)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
ARL13B-(Y86C)-GFP

Plasmid

#246840

Purpose

Express Arl13B (resistance to sgRNA), tagged with GFP, with Y86C mutation

Depositor

Lei Lu

Article

Mahajan et al Sci Rep. 2025 Jul 24;15(1):26980. doi: 1

Insert

Arl13B (ARL13B Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

changed Tyrosine 86 to Cysteine

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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ARL13B-(G75Q)-GFP

Plasmid

#246837

Purpose

Express Arl13B (resistance to sgRNA), tagged with GFP, with G75Q mutation

Depositor

Lei Lu

Article

Mahajan et al Sci Rep. 2025 Jul 24;15(1):26980. doi: 1

Insert

Arl13b (ARL13B Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

changed G 75 to Q

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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ARL13B-R-GFP

Plasmid

#246831

Purpose

Express Arl13B-GFP in Arl13B KO RPE1 cells (sg1#a), resistance to sgRNA

Depositor

Lei Lu

Article

Mahajan et al Sci Rep. 2025 Jul 24;15(1):26980. doi: 1

Insert

Arl13b-R-GFP (ARL13B Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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ARL13B-(R79Q)-GFP

Plasmid

#246839

Purpose

Express Arl13B (resistance to sgRNA), tagged with GFP, with R79Q mutation

Depositor

Lei Lu

Article

Mahajan et al Sci Rep. 2025 Jul 24;15(1):26980. doi: 1

Insert

Arl13B (ARL13B Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

changed Arginine 79 to Glutamine

Available Since

Dec. 4, 2025

Availability

Academic Institutions and Nonprofits only

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ARL13B-(R200C)-GFP

Plasmid

#246841

Purpose

Express Arl13B (resistance to sgRNA), tagged with GFP, with R200C mutation

Depositor

Lei Lu

Article

Mahajan et al Sci Rep. 2025 Jul 24;15(1):26980. doi: 1

Insert

Arl13B (ARL13B Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

changed Arginine 200 to Cysteine

Available Since

Dec. 4, 2025

Availability

Academic Institutions and Nonprofits only

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